公开(公告)号：US06828436B2

公开(公告)日：2004-12-07

申请号：US10146266

申请日：2002-05-15

申请人： Abhinav A. Shukla ,  Ranjit R. Deshmukh ,  Steven M. Cramer ,  James A. Moore

发明人： Abhinav A. Shukla ,  Ranjit R. Deshmukh ,  Steven M. Cramer ,  James A. Moore

IPC分类号： C07H2100

CPC分类号： C12N15/101 ,  B01D15/422

摘要： A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.

摘要翻译： 公开了使用高亲和力，低分子量（小于约10000Da）置换剂在阴离子交换介质上通过置换色谱法纯化寡核苷酸的方法。 提供了高亲和性，低分子量阴离子置换剂的几个实例，包括其上连接有磺酸基团的多环芳族化合物。 该技术用于高分辨率分离寡核苷酸的功效证明了工业混合物。

公开(公告)号：US06573373B1

公开(公告)日：2003-06-03

申请号：US09343006

申请日：1999-06-29

申请人： Abhinav A. Shukla ,  Ranjit R. Deshmukh ,  Steven M. Cramer ,  James A. Moore

发明人： Abhinav A. Shukla ,  Ranjit R. Deshmukh ,  Steven M. Cramer ,  James A. Moore

IPC分类号： C07H2100

CPC分类号： C12N15/101 ,  B01D15/422

摘要： A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.