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公开(公告)号：US20090306351A1
公开(公告)日：2009-12-10
申请号：US12225216
申请日：2007-03-16
申请人： Abhinav A. Shukla , Peter Hinckley
发明人： Abhinav A. Shukla , Peter Hinckley
IPC分类号： C07K1/22
CPC分类号： C07K1/22 , B01D15/20 , B01D15/3809
摘要： A method for purifying a protein using Protein A chromatography comprising a) absorbing the protein to Protein A immobilized on a solid support; b) removing contaminants by washing the immobilized Protein A containing the absorbed protein with a buffer comprising one or more chaotropic agents in combination with one or more hydrophobic modifiers and having a pH of at least 7.0; and c) eluting the protein from the Protein A immobilized on the solid support.
摘要翻译： 一种使用蛋白A色谱法纯化蛋白质的方法，包括a）将蛋白质吸收固定在固体支持物上的蛋白A; b）通过用包含一种或多种离液剂的缓冲液与一种或多种疏水性改性剂组合并且具有至少7.0的pH洗涤含有所述吸收的蛋白质的固定化蛋白A来去除污染物; 和c）从固定在固体支持物上的蛋白A洗脱蛋白质。
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2.发明授权Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction 有权在疏水相互作用层析流通级分中纯化蛋白质的方法公开(公告)号：US07427659B2
公开(公告)日：2008-09-23
申请号：US10970860
申请日：2004-10-22
CPC分类号： C07K14/00 , C07K1/20 , C07K2319/30
摘要： The present invention is a process for separating a target protein (such as a recombinant protein produced in a cell culture) from a mixture containing the target protein and contaminants (such as cell culture contaminants), by contacting the mixture with a hydrophobic adsorbent comprising branched hydrocarbon functional groups in an aqueous salt solution and collecting the unbound flow-through fraction containing the target protein. In one embodiment, the hydrophobic adsorbent may be a branched alkyl functional group. In another embodiment, the branched alkyl functional group has from 3 to 8 carbon atoms. In another embodiment, the branched alkyl functional group is a tertiary carbon atom, such as tert-butyl.
摘要翻译： 本发明是将含有目标蛋白质和污染物（例如细胞培养污染物）的混合物的目标蛋白质（例如细胞培养物中产生的重组蛋白质）分离出来的方法，该方法是将含有支链 烃盐官能团，并收集含有目标蛋白质的未结合的流通级分。 在一个实施方案中，疏水性吸附剂可以是支链烷基官能团。 在另一个实施方案中，支链烷基官能团具有3至8个碳原子。 在另一个实施方案中，支链烷基官能团是叔碳原子，例如叔丁基。
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3.发明授权Method for selecting a high affinity, low molecular weight displacer for oligonucleotide purification 失效选择高亲和力，低分子量置换剂用于寡核苷酸纯化的方法公开(公告)号：US06828436B2
公开(公告)日：2004-12-07
申请号：US10146266
申请日：2002-05-15
IPC分类号： C07H2100
CPC分类号： C12N15/101 , B01D15/422
摘要： A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.
摘要翻译： 公开了使用高亲和力，低分子量（小于约10000Da）置换剂在阴离子交换介质上通过置换色谱法纯化寡核苷酸的方法。 提供了高亲和性，低分子量阴离子置换剂的几个实例，包括其上连接有磺酸基团的多环芳族化合物。 该技术用于高分辨率分离寡核苷酸的功效证明了工业混合物。
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4.发明授权Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step 有权使用中间洗涤步骤的蛋白A亲和层析纯化蛋白质的方法公开(公告)号：US08263750B2
公开(公告)日：2012-09-11
申请号：US12225216
申请日：2007-03-16
申请人： Abhinav A. Shukla , Peter Hinckley
发明人： Abhinav A. Shukla , Peter Hinckley
CPC分类号： C07K1/22 , B01D15/20 , B01D15/3809
摘要： A method for purifying a protein using Protein A chromatography comprising a) absorbing the protein to Protein A immobilized on a solid support; b) removing contaminants by washing the immobilized Protein A containing the absorbed protein with a buffer comprising one or more chaotropic agents in combination with one or more hydrophobic modifiers and having a pH of at least 7.0; and c) eluting the protein from the Protein A immobilized on the solid support.
摘要翻译： 一种使用蛋白A色谱法纯化蛋白质的方法，包括a）将蛋白质吸收固定在固体支持物上的蛋白A; b）通过用包含一种或多种离液剂的缓冲液与一种或多种疏水性改性剂组合并且具有至少7.0的pH洗涤含有所述吸收的蛋白质的固定化蛋白A来去除污染物; 和c）从固定在固体支持物上的蛋白A洗脱蛋白质。
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公开(公告)号：US06573373B1
公开(公告)日：2003-06-03
申请号：US09343006
申请日：1999-06-29
IPC分类号： C07H2100
CPC分类号： C12N15/101 , B01D15/422
摘要： A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed. Several examples of high affinity, low molecular weight anionic displacers are provided, including polycyclic aromatic compounds having sulfonic acid moieties attached thereon. The efficacy of the technique for high resolution separation of oligonucleotides is demonstrated for an industrial mixture.
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6.发明授权Low molecular weight displacers for protein purification in hydrophobic interaction and reversed phase chromatographic systems 有权用于疏水相互作用和反相色谱系统中蛋白质纯化的低分子量置换剂公开(公告)号：US06239262B1
公开(公告)日：2001-05-29
申请号：US09223093
申请日：1998-12-30
IPC分类号： C07K114
CPC分类号： C12N9/2462 , B01D15/325 , B01D15/327 , B01D15/422 , C07K14/475 , C07K14/76
摘要： A method for purification of proteins by displacement chromatography in hydrophobic interaction and reversed phase chromatographic systems uses low molecular weight (less than about 10,000) surface-active compounds as displacers. Examples of effective displacers are benzethonium chloride, benzyltributylammonium chloride, and tetrahexylammonium chloride.
摘要翻译： 在疏水相互作用和反相色谱系统中通过置换色谱法纯化蛋白质的方法使用低分子量（少于约10,000）表面活性化合物作为置换剂。 有效置换剂的实例是苄索氯铵，苄基三丁基氯化铵和四己基氯化铵。
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