公开(公告)号：US09228196B2

公开(公告)日：2016-01-05

申请号：US14067317

申请日：2013-10-30

Applicant: Academia Sinica

Inventor： Yi-Fang Tsay ,  Shu-Chun Fan ,  Hui-Yu Chen ,  Kuo-En Chen

IPC: C12N5/14 ,  C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8243 ,  C07K14/415 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention provides a method for changing nitrogen utilization efficiency in a plant comprises regulating the expression of Arabidopsis NRT 1.7 or an ortholog thereof so that the nitrate remobilization from older leaves to young leaves in the plant is regulated, thereby the nitrogen utilization efficiency is changed. The present invention also provides a transgenic plant obtainable by transforming a plant with an expression construct with a high or low level of expression of NRT 1.7. On the other hand, the present invention yet provides a chimera nitrate transporter, a DNA molecule coding for this chimera transporter and an expression vector thereof.

Abstract translation: 本发明提供一种改变植物氮利用效率的方法，包括调节拟南芥NRT 1.7或其直系同源物的表达，从而调节植物中从老叶到幼叶的硝酸盐再生，从而改变氮利用效率 。 本发明还提供了通过用具有高或低水平的NRT1.7表达的表达构建体转化植物可获得的转基因植物。 另一方面，本发明还提供了嵌合体硝酸盐转运蛋白，编码该嵌合体转运蛋白的DNA分子及其表达载体。

公开(公告)号：US20140201863A1

公开(公告)日：2014-07-17

申请号：US14067317

申请日：2013-10-30

Applicant: Academia Sinica

Inventor： Yi-Fang Tsay ,  Shu-Chun Fan ,  Hui-Yu Chen

IPC: C12N15/82

CPC classification number: C12N15/8243 ,  C07K14/415 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention provides a method for changing nitrogen utilization efficiency in a plant comprises regulating the expression of Arabidopsis NRT 1.7 or an orthologue thereof so that the nitrate remobilization from older leaves to young leaves in the plant is regulated, thereby the nitrogen utilization efficiency is changed. The present invention also provides a transgenic plant obtainable by transforming a plant with an expression construct with a high or low level of expression of NRT 1.7. On the other hand, the present invention yet provides a chimera nitrate transporter, a DNA molecule coding for this chimera transporter and an expression vector thereof.

Abstract translation: 本发明提供一种改变植物氮利用效率的方法，包括调节拟南芥NRT 1.7或其直向同源物的表达，从而调节植物中从老叶到幼叶的硝酸盐再生，从而改变氮利用效率 。 本发明还提供了通过用具有高或低水平的NRT1.7表达的表达构建体转化植物可获得的转基因植物。 另一方面，本发明还提供了嵌合体硝酸盐转运蛋白，编码该嵌合体转运蛋白的DNA分子及其表达载体。