公开(公告)号：US12109309B2

公开(公告)日：2024-10-08

申请号：US16322014

申请日：2017-07-28

申请人： Academia Sinica

发明人： Che-Ming Jack Hu ,  Hui-Wen Chen ,  Yuan-I Chen ,  Chen-Ying Chien ,  Jung-Chen Lin

IPC分类号： A61K9/127 ,  C12N5/078 ,  G01N33/50 ,  G01N33/543 ,  G01N33/556

CPC分类号： A61K9/127 ,  A61K9/1275 ,  A61K9/1277 ,  C12N5/0634 ,  G01N33/5008 ,  G01N33/5432 ,  G01N33/556 ,  G01N2500/02 ,  G01N2500/10

摘要： A method of generating an internally fixed lipid vesicle, comprising: providing a precursor lipid vesicle that contains an aqueous interior enclosed by a lipid membrane, wherein the lipid membrane of the precursor lipid vesicle is non-permeable to a crosslinker; permeabilizing the lipid membrane transiently to generate a permeable vesicle; contacting the permeable vesicle with an inactive activatable crosslinker, whereby the inactive activatable crosslinker enters the permeable vesicle; allowing the permeable vesicle to return to a non-permeable vesicle; removing any extravesicular crosslinker; and activating the inactive activatable crosslinker to allow crosslinking to occur inside the non-permeable vesicle, whereby an internally fixed lipid vesicle is generated.