公开(公告)号：US20160313316A1

公开(公告)日：2016-10-27

申请号：US15103477

申请日：2014-12-10

Applicant: Agency for Science, Technology and Research

Inventor： Kui YAO ,  Chin Yaw TAN ,  Ying JIANG ,  Yi Fan CHEN ,  Sze Yu TAN ,  Lei ZHANG

IPC: G01N33/543 ,  G01N29/02 ,  G01N33/569 ,  G01N29/32

CPC classification number: G01N33/54366 ,  G01N29/022 ,  G01N29/22 ,  G01N29/326 ,  G01N33/56983 ,  G01N2291/02466 ,  G01N2291/0256 ,  G01N2291/0257 ,  G01N2333/11

Abstract: An influenza detector for detecting a targeted influenza virus and a surface acoustic wave (SAW) sensor for Influenza A virus detection in liquid are provided. The influenza detector includes a liquid environment, the surface acoustic wave (SAW) sensor and an influenza specific binding agent such as an antibody. The agent is immobilized on a surface of the SAW sensor for selectively capturing an analyte for the targeted influenza virus. The SAW sensor is in contact with the liquid environment and includes a substrate comprising a piezo-electric material for producing a surface acoustic wave signal in response to an applied electric field and an insulative layer formed on top of the substrate and having a functionalized surface formed thereon for selectively immobilizing the influenza specific binding agent, the functionalized surface being in contact with the liquid environment. The surface acoustic wave signal produced by the SAW sensor changes in response to the analyte for the targeted influenza virus being present in the liquid environment and being captured by the influenza specific binding agent immobilized on the functionalized surface of the insulative layer of the SAW sensor.

Abstract translation: 提供用于检测目标流感病毒的流感检测器和用于液体中甲型流感病毒检测的表面声波（SAW）传感器。 流感检测器包括液体环境，表面声波（SAW）传感器和流感特异性结合剂如抗体。 将该试剂固定在SAW传感器的表面上，以选择性地捕获目标流感病毒的分析物。 SAW传感器与液体环境接触，并且包括基板，该基板包括用于响应于所施加的电场产生表面声波信号的压电材料和形成在基板的顶部上的绝缘层，并且具有形成的功能化表面 其上用于选择性地固定流感特异性结合剂，所述官能化表面与液体环境接触。 由SAW传感器产生的表面声波信号响应于目标流感病毒的分析物存在于液体环境中并被固定在SAW传感器的绝缘层的功能化表面上的流感特异性结合剂捕获而变化。