公开(公告)号：US20240335589A1

公开(公告)日：2024-10-10

申请号：US18294106

申请日：2022-08-04

Applicant: Agency for Science, Technology and Research

Inventor： Deepak Choudhury ,  Jia Sheng Lee ,  Ahmad Amirul ,  May Win Naing

IPC: A61L27/36

CPC classification number: A61L27/3633 ,  A61L27/3683 ,  A61L2430/40

Abstract: The invention relates to a method for decellularization of tissue samples to produce decellularized extracellular matrix (dECM) by performing one or more wash cycles in a reactor using a reagent after at least partially submerging the tissue sample in the reagent, wherein the reactor comprising a tissue chamber in fluid communication with a reactor chamber. The method further comprising the steps of agitating the tissue sample and/or the reagent such that the tissue sample circulates in the reagent while retaining the tissue sample in a tissue chamber or a decellularization reactor, monitoring at least one parameter of the reagent and/or at least one parameter of the tissue sample, and adjusting one or more wash cycles based on said monitoring. The invention further relates to a decellularization reactor and a decellularization system for decellularizing a tissue sample.

公开(公告)号：US20150284669A1

公开(公告)日：2015-10-08

申请号：US14441831

申请日：2012-11-08

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH ,  NATIONAL UNIVERSITY OF SINGAPORE

Inventor： Deepak Choudhury ,  Chukwuemeka Anene-Nzelu ,  Hanry Yu ,  Yi-Chin Toh ,  Hwa Liang Leo ,  Sum Huan Ng

IPC: C12M1/12

CPC classification number: C12M25/06 ,  C12N2535/10

Abstract: A method of culturing cells or tissues is provided. The method comprises a) providing a support, wherein the support is an optical medium with a patterned surface; b) contacting the support with cells and a culture medium; and c) culturing the cells under suitable cultivation conditions. A device for cell or tissue culture comprising an optical medium, wherein a patterned surface of the optical medium forms a support upon which cells are cultivated, is also provided.

Abstract translation: 提供培养细胞或组织的方法。 该方法包括：a）提供支撑件，其中所述支撑件是具有图案化表面的光学介质; b）使所述载体与细胞和培养基接触; 和c）在合适的培养条件下培养细胞。 一种用于细胞或组织培养的装置，其包括光学介质，其中光学介质的图案化表面形成培养细胞的载体。

公开(公告)号：US20150223433A1

公开(公告)日：2015-08-13

申请号：US14619649

申请日：2015-02-11

Applicant: Agency for Science, Technology and Research

Inventor： Rapeechai Navawongse ,  Deepak Choudhury ,  Adam Claridge-Chang ,  Zhiping Wang

IPC: A01K29/00 ,  G06T7/00 ,  G01F23/00 ,  G06K9/00 ,  A01K1/03 ,  A01K5/00

CPC classification number: A01K29/005 ,  A01K1/031 ,  A01K67/033 ,  G06K9/00335 ,  G06K9/00362

Abstract: A method of monitoring activity of an animal, the method comprising the steps of: retaining the animal in an enclosed chamber; controlling a food supply to the animal in the enclosed chamber as a first stimulus; and monitoring the activity of the animal in response to the first stimulus, wherein monitoring the activity of the animal comprises analysing change in a volume of food in the food supply and location of the animal in the enclosed chamber over a predetermined period of time.

Abstract translation: 一种监测动物活动的方法，所述方法包括以下步骤：将动物保持在封闭的室内; 控制在封闭室内的动物的食物供应作为第一刺激; 以及响应于所述第一刺激来监测动物的活动，其中监测动物的活动包括在预定时间段内分析所述食物供应中食物的量的变化和所述封闭室中的动物的位置。