公开(公告)号：US11237159B2

公开(公告)日：2022-02-01

申请号：US16086285

申请日：2017-03-23

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Ghayathri Balasundaram

IPC: G01N33/543 ,  B01L3/00 ,  G01N21/65 ,  G01N33/574

Abstract: A biosensor for the detection of an analyte using surface-enhanced Raman spectroscopy (SERS) is provided. The biosensor includes a SERS-active substrate and a microfludic circuit device arranged to be in fluid communication with the SERS-active substrate. Method of manufacturing a biosensor, and methods for detecting an analyte using the biosensor, wherein the analyte may be haptoglobin, are also provided.

公开(公告)号：US20190056328A1

公开(公告)日：2019-02-21

申请号：US16078273

申请日：2017-02-22

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Ghayathri Balasundaram

IPC: G01N21/65 ,  G01N33/543 ,  G01N33/574

Abstract: According to the present disclosure, a method for detecting an analyte using surface enhanced Raman spectroscopy (SERS) is provided. The method comprises (a) contacting one or more analyte-binding molecules with the analyte under conditions that allow binding of the analyte to the one or more analyte-binding molecules to form a first mixture, wherein the analyte is preferably haptogloblin and the analyte-binding molecule may comprise haemoglobin or is a haptogloblin antibody, (b) contacting a liquid reagent comprising a peroxidase substrate and a peroxide source with the first mixture to form a second mixture, while maintaining pH of the second mixture at 10 or less, (c) quenching the second mixture to form a third mixture, (d) optionally contacting the third mixture with a SERS-active substrate, and (e) detecting a surface enhanced Raman signal from the third mixture and/or a surface of the SERS-active substrate.

公开(公告)号：US11493448B2

公开(公告)日：2022-11-08

申请号：US16078273

申请日：2017-02-22

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Ghayathri Balasundaram

IPC: G01N21/65 ,  G01N33/543 ,  G01N33/574

Abstract: According to the present disclosure, a method for detecting an analyte using surface enhanced Raman spectroscopy (SERS) is provided. The method comprises (a) contacting one or more analyte-binding molecules with the analyte under conditions that allow binding of the analyte to the one or more analyte-binding molecules to form a first mixture, wherein the analyte is preferably haptogloblin and the analyte-binding molecule may comprise haemoglobin or is a haptogloblin antibody, (b) contacting a liquid reagent comprising a peroxidase substrate and a peroxide source with the first mixture to form a second mixture, while maintaining pH of the second mixture at 10 or less, (c) quenching the second mixture to form a third mixture, (d) optionally contacting the third mixture with a SERS-active substrate, and (e) detecting a surface enhanced Raman signal from the third mixture and/or a surface of the SERS-active substrate.

公开(公告)号：US20220162663A1

公开(公告)日：2022-05-26

申请号：US17602271

申请日：2020-04-08

Applicant: Agency for Science, Technology and Research ,  Matralix Pte Ltd

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Renuga Gopal ,  Saif Abdul Kadir Khan

IPC: C12Q1/02 ,  G01N21/65 ,  G01N1/40

Abstract: A method of identifying a Mycobacterium using surface enhanced Raman spectroscopy (SERS), disclosed herein, comprises disposing a sample suspected to comprise a Mycobacterium on a SERS-active substrate, detecting surface enhanced Raman signals corresponding to an alpha-mycolic acid, a methoxy-mycolic acid, and a keto-mycolic acid from the sample disposed on the SERS-active substrate, and determining one or more ratios of intensity of the surface enhanced Raman signals to identify the Mycobacterium. Disclosed herein also includes a method of detecting a mycobacterial disease using surface enhanced Raman spectroscopy (SERS), the method comprising identifying a Mycobacterium according to the method described above, and determining the mycobacterial disease based on the Mycobacterium identified.

公开(公告)号：US20240353339A1

公开(公告)日：2024-10-24

申请号：US18683345

申请日：2022-08-19

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Dinish Unnimadhava Kurup Soudamini Amma

IPC: G01N21/65 ,  G01N33/543 ,  G01N33/68

CPC classification number: G01N21/658 ,  G01N33/54346 ,  G01N33/54373 ,  G01N33/6893 ,  G01N2333/525 ,  G01N2333/54 ,  G01N2333/96419

Abstract: A biocompatible SERS-active polymer membrane configured to detect biomarkers in a sample. The biocompatible SERS-active polymer membrane includes a flexible and porous polymer membrane and SERS-active nanoparticles formed on the flexible and porous polymer membrane, where the flexible and porous polymer membrane includes cellulose or an elastomeric polymer. Also disclosed herein is a method of forming the biocompatible SERS-active polymer membrane and a method of determining a state of wound healing in a diabetic individual involving the use of the biocompatible SERS-active polymer membrane.

公开(公告)号：US20230175968A1

公开(公告)日：2023-06-08

申请号：US17922349

申请日：2021-04-30

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Mohesh Moothanchery ,  Gurpreet Singh

IPC: G01N21/65

CPC classification number: G01N21/65 ,  G01N2201/0221 ,  G01N2201/06113 ,  G01N2021/0106

Abstract: A portable device which detects a Raman signal from an analyte of interest contained in or suspected to contain in a sample. The portable device includes a laser source in optical communication with a dichroic mirror, an objective lens optically positioned to consolidate the laser from the laser source to the sample, (i) a pair of rotatable filters in optical communication with the dichroic mirror and an optical transmission module or (ii) a first band-pass filter in optical communication with the dichroic mirror and an optical transmission module, and a detector optically positioned to receive and detect a Raman signal produced from the laser incident on the analyte of interest. The dichroic mirror directs the laser from the laser source to the sample through the objective lens and transmits any Raman signal from the sample toward the detector. The detector is absent of a spectrometer and detects the Raman signal.

公开(公告)号：US20200292538A1

公开(公告)日：2020-09-17

申请号：US16086285

申请日：2017-03-23

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Ghayathri Balasundaram

IPC: G01N33/543 ,  G01N21/65 ,  B01L3/00 ,  G01N33/574

Abstract: A biosensor for the detection of an analyte using surface-enhanced Raman spectroscopy (SERS) is provided. The biosensor includes a SERS-active substrate and a microfludic circuit device arranged to be in fluid communication with the SERS-active substrate. Method of manufacturing a biosensor, and methods for detecting an analyte using the biosensor, wherein the analyte may be haptoglobin, are also provided.