公开(公告)号：US11237159B2

公开(公告)日：2022-02-01

申请号：US16086285

申请日：2017-03-23

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Ghayathri Balasundaram

IPC: G01N33/543 ,  B01L3/00 ,  G01N21/65 ,  G01N33/574

Abstract: A biosensor for the detection of an analyte using surface-enhanced Raman spectroscopy (SERS) is provided. The biosensor includes a SERS-active substrate and a microfludic circuit device arranged to be in fluid communication with the SERS-active substrate. Method of manufacturing a biosensor, and methods for detecting an analyte using the biosensor, wherein the analyte may be haptoglobin, are also provided.

公开(公告)号：US11493448B2

公开(公告)日：2022-11-08

申请号：US16078273

申请日：2017-02-22

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Ghayathri Balasundaram

IPC: G01N21/65 ,  G01N33/543 ,  G01N33/574

Abstract: According to the present disclosure, a method for detecting an analyte using surface enhanced Raman spectroscopy (SERS) is provided. The method comprises (a) contacting one or more analyte-binding molecules with the analyte under conditions that allow binding of the analyte to the one or more analyte-binding molecules to form a first mixture, wherein the analyte is preferably haptogloblin and the analyte-binding molecule may comprise haemoglobin or is a haptogloblin antibody, (b) contacting a liquid reagent comprising a peroxidase substrate and a peroxide source with the first mixture to form a second mixture, while maintaining pH of the second mixture at 10 or less, (c) quenching the second mixture to form a third mixture, (d) optionally contacting the third mixture with a SERS-active substrate, and (e) detecting a surface enhanced Raman signal from the third mixture and/or a surface of the SERS-active substrate.

公开(公告)号：US20200292538A1

公开(公告)日：2020-09-17

申请号：US16086285

申请日：2017-03-23

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Ghayathri Balasundaram

IPC: G01N33/543 ,  G01N21/65 ,  B01L3/00 ,  G01N33/574

Abstract: A biosensor for the detection of an analyte using surface-enhanced Raman spectroscopy (SERS) is provided. The biosensor includes a SERS-active substrate and a microfludic circuit device arranged to be in fluid communication with the SERS-active substrate. Method of manufacturing a biosensor, and methods for detecting an analyte using the biosensor, wherein the analyte may be haptoglobin, are also provided.

公开(公告)号：US10398315B2

公开(公告)日：2019-09-03

申请号：US15038029

申请日：2014-12-08

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jun Hui Ho ,  Dinish Unnimadhava Kurup Soudamini Amma ,  Chi Lok Wong ,  Ghayathri Balasundaram

IPC: A61B5/00 ,  A61B8/08 ,  A61K49/00 ,  A61K49/22 ,  G01N33/483

Abstract: A method of imaging living tissue is provided. The method includes introducing a photoacoustic contrast agent comprising or consisting of a photosensitizer into living tissue; and obtaining an image of the living tissue by photoacoustic imaging. Use of a photoacoustic contrast agent comprising or consisting of a photosensitizer in photoacoustic imaging is also provided.

公开(公告)号：US20190056328A1

公开(公告)日：2019-02-21

申请号：US16078273

申请日：2017-02-22

Applicant: Agency for Science, Technology and Research

Inventor： Malini Olivo ,  Jayakumar Perumal ,  Ghayathri Balasundaram

IPC: G01N21/65 ,  G01N33/543 ,  G01N33/574

Abstract: According to the present disclosure, a method for detecting an analyte using surface enhanced Raman spectroscopy (SERS) is provided. The method comprises (a) contacting one or more analyte-binding molecules with the analyte under conditions that allow binding of the analyte to the one or more analyte-binding molecules to form a first mixture, wherein the analyte is preferably haptogloblin and the analyte-binding molecule may comprise haemoglobin or is a haptogloblin antibody, (b) contacting a liquid reagent comprising a peroxidase substrate and a peroxide source with the first mixture to form a second mixture, while maintaining pH of the second mixture at 10 or less, (c) quenching the second mixture to form a third mixture, (d) optionally contacting the third mixture with a SERS-active substrate, and (e) detecting a surface enhanced Raman signal from the third mixture and/or a surface of the SERS-active substrate.

公开(公告)号：US20160310010A1

公开(公告)日：2016-10-27

申请号：US15038029

申请日：2014-12-08

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Malini Olivo ,  Jun Hui Ho ,  Dinish Unnimadhava Kurup Soudamini Amma ,  Chi Lok Wong ,  Ghayathri Balasundaram

IPC: A61B5/00 ,  A61K49/22 ,  G01N33/483 ,  A61B8/08

CPC classification number: A61B5/0095 ,  A61B8/481 ,  A61K49/003 ,  A61K49/0036 ,  A61K49/0093 ,  A61K49/22 ,  G01N33/4833

Abstract: A method of imaging living tissue is provided. The method includes introducing a photoacoustic contrast agent comprising or consisting of a photosensitizer into living tissue; and obtaining an image of the living tissue by photoacoustic imaging. Use of a photoacoustic contrast agent comprising or consisting of a photosensitizer in photoacoustic imaging is also provided.

Abstract translation: 提供了一种生物组织成像方法。 该方法包括将包含光敏剂或由光敏剂组成的光声造影剂引入活组织; 并通过光声成像获得活体组织的图像。 还提供了光声成像中包含光敏剂或由光敏剂组成的光声造影剂的使用。