公开(公告)号：US10544458B2

公开(公告)日：2020-01-28

申请号：US15552021

申请日：2016-02-24

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Somenath Roy ,  Jun Hui Soh

IPC: C12Q1/6876

Abstract: The present invention relates to a microarray device for detecting a target molecule such as miRNA in a sample. The device comprises a carrier substrate such as glass, an anti-fouling polymer layer which is functionalised with N-Hydroxysuccinimide (NHS) or carboxyl-groups and a capture probe. In an embodiment, the capture probe is an oligonucleotide with a stem-loop structure. The invention further defines a method for fabricating the device, a kit for detecting the target nucleic acid molecule comprising the device and a detection probe.

公开(公告)号：US20180216181A1

公开(公告)日：2018-08-02

申请号：US15552021

申请日：2016-02-24

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Somenath Roy ,  Jun Hui Soh

IPC: C12Q1/6876

Abstract: The present invention relates to a microarray device for detecting a target molecule such as miRNA in a sample. The device comprises a carrier substrate such as glass, an anti-fouling polymer layer which is functionalised with N-Hydroxysuccinimide (NHS) or carboxyl-groups and a capture probe. In an embodiment, the capture probe is an oligonucleotide with a stem-loop structure. The invention further defines a method for fabricating the device, a kit for detecting the target nucleic acid molecule comprising the device and a detection probe.