公开(公告)号：US11873508B2

公开(公告)日：2024-01-16

申请号：US16473266

申请日：2018-01-22

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jackie Y. Ying ,  Leng Leng Chng ,  Daniele Zink ,  Aysha Farwin

IPC: C12N5/00 ,  C08F293/00 ,  C08L53/00 ,  C12N5/0775

CPC classification number: C12N5/0075 ,  C08F293/005 ,  C08L53/005 ,  C12N5/0662 ,  C08F2438/00 ,  C08F2810/20 ,  C08F2810/50 ,  C08L2207/53 ,  C08L2312/00 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2531/00 ,  C12N2533/30 ,  C12N2539/10

Abstract: There is provided a polymeric microsphere comprising a thermally responsive monomer crosslinked with a functional group monomer, wherein the functional group monomer comprises at least one of a carboxylic acid functional group or an amine functional group. The thermally responsive monomer is preferably N-isopropylacrylamide (NIPAM), and the microspheres preferably comprise a coating of polymerized catecholamines (e.g. DOPA). There is also provided a method of preparing the polymeric microsphere and uses of the polymeric microsphere in culturing, harvesting, or expanding stem cells or stromal cells. Preferably, the cells, e.g. hMSCs (human mesenchymal stem/stromal cells), are expanded or harvested in serum-free and xeno-free medium.

公开(公告)号：US11701657B2

公开(公告)日：2023-07-18

申请号：US17077828

申请日：2020-10-22

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Min-Han Tan ,  Yoke San Daniel Lee ,  Igor Cima ,  Yeon Joon Park ,  Wai Min Phyo

IPC: B01L3/00 ,  C12N5/071 ,  G01N33/50 ,  G01N33/574 ,  C12Q1/6886

CPC classification number: B01L3/502761 ,  C12N5/069 ,  C12Q1/6886 ,  G01N33/5064 ,  G01N33/5094 ,  G01N33/574 ,  G01N33/57419 ,  G01N33/57492 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2300/042 ,  B01L2300/0681 ,  C12Q2600/156

Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.

公开(公告)号：US11142790B2

公开(公告)日：2021-10-12

申请号：US15302974

申请日：2015-03-30

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jackie Y. Ying ,  Guolin Xu ,  Rensheng Deng

IPC: C12Q1/68 ,  G01N21/64 ,  C12Q1/686 ,  B01L3/00 ,  G01N33/543

Abstract: There is provided a microfluidic device comprising: a plurality of wells, each well comprising one opening to function as an inlet and an outlet for the well, wherein each opening is in fluid communication with a common fluidic channel, and wherein each opening is connected to the common fluidic channel via an isolation channel, and wherein the plurality of wells is arranged on the device in a radially symmetrical pattern. There is also provided a system and method comprising the device.

公开(公告)号：US11084034B2

公开(公告)日：2021-08-10

申请号：US15544761

申请日：2016-01-21

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Min-Han Tan ,  Yoke San Daniel Lee ,  Igor Cima ,  Yeon Joon Park ,  Wai Min Phyo

IPC: C12N5/071 ,  G01N33/50 ,  G01N33/574 ,  B01L3/00 ,  C12Q1/6886

Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.

公开(公告)号：US10975350B2

公开(公告)日：2021-04-13

申请号：US15780236

申请日：2016-11-30

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jackie Y. Ying ,  Nandanan Erathodiyil ,  Karthikeyan Narayanan ,  Andrew C. A. Wan

IPC: C12N5/00 ,  C12N5/0775 ,  B82Y30/00 ,  B82Y5/00

Abstract: A cell culture substrate comprising a substrate having a coating of a plurality of amine functionalized nanoparticles is disclosed. In one embodiment, the amine functionalized nanoparticle is a polymer of an acrylamide monomer, a cross-linker and an amine monomer. There is also provided a method of making the cell culture substrate either by drying the amine functionalized nanoparticles when spread onto the said substrate or by covalent linkages of the substrate with thiol terminated nanoparticles. In addition, there is provided a method of culturing stem cells on the cell culture substrate having a coating of a plurality of the amine functionalized nanoparticles thereon.

公开(公告)号：US10287553B2

公开(公告)日：2019-05-14

申请号：US14395811

申请日：2013-04-22

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Daniele Zink ,  Ming Ni ,  Karthikeyan Narayanan ,  Karthikeyan Kandasamy ,  Andrew C. A. Wan ,  Jackie Y. Ying

IPC: C12N5/074 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/0775

Abstract: There is provided a method for culturing a stem cell in vitro. The method comprises providing a substrate surface coated with a coating comprising a molecule having a catechol moiety or a polymer thereof; and growing a stem cell on said coated substrate surface in a growth medium.

公开(公告)号：US20190024175A1

公开(公告)日：2019-01-24

申请号：US16032707

申请日：2018-07-11

Applicant: Agency for Science, Technology and Research

Inventor： Yanbing Zu ,  Jackie Y. Ying

IPC: C12Q1/6883 ,  G01N21/78 ,  C12Q1/6827 ,  C12Q1/6881

CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N21/78 ,  C12Q2525/113 ,  C12Q2527/107 ,  C12Q2563/149

Abstract: A method and kit for detecting a genetic variant associated with a disease or disorder, including incompatibility with a pharmaceutical. The method and kit using a first nano-particle coupled to at least one morpholino nucleic acid probe comprising a target complimentary region base sequence that is a perfect match to a genetic variant sequence.

公开(公告)号：US20180216181A1

公开(公告)日：2018-08-02

申请号：US15552021

申请日：2016-02-24

Applicant: Agency for Science, Technology and Research

Inventor： Jackie Y. Ying ,  Somenath Roy ,  Jun Hui Soh

IPC: C12Q1/6876

Abstract: The present invention relates to a microarray device for detecting a target molecule such as miRNA in a sample. The device comprises a carrier substrate such as glass, an anti-fouling polymer layer which is functionalised with N-Hydroxysuccinimide (NHS) or carboxyl-groups and a capture probe. In an embodiment, the capture probe is an oligonucleotide with a stem-loop structure. The invention further defines a method for fabricating the device, a kit for detecting the target nucleic acid molecule comprising the device and a detection probe.

公开(公告)号：US20170258757A1

公开(公告)日：2017-09-14

申请号：US15598407

申请日：2017-05-18

Applicant: Agency for Science, Technology and Research

Inventor： Motoichi Kurisawa ,  Kun Liang ,  Susi Tan ,  Joo Eun Chung ,  Jackie Y. Ying

IPC: A61K31/282 ,  A61K9/107 ,  A61K31/353 ,  A61K31/704 ,  A61K31/7088 ,  A61K45/06 ,  A61K9/00 ,  A61K31/337

CPC classification number: A61K31/282 ,  A61K9/0019 ,  A61K9/1075 ,  A61K31/337 ,  A61K31/353 ,  A61K31/704 ,  A61K31/7088 ,  A61K45/06 ,  A61K47/60 ,  A61K2300/00

Abstract: There is provided a conjugate of a delivery agent containing a chemical moiety and at least one flavonoid. The flavonoid exists in a monomeric form or dimeric form before conjugation and remains in the monomeric form or dimeric form after conjugation. Preferably, the conjugate comprises two flavonoids. The delivery agent is conjugated at the C6 and/or the C8 position of the A ring of the flavonoid. An anti-cancer agent delivery vehicle comprising an anti-cancer agent and the conjugate is also provided.

公开(公告)号：US20170158837A1

公开(公告)日：2017-06-08

申请号：US15382675

申请日：2016-12-18

Applicant: Agency for Science, Technology and Research

Inventor： Yugen Zhang ,  Jackie Y. Ying ,  Mei Xuan Tan

IPC: C08J9/28 ,  C08G12/32 ,  B01J20/26 ,  B01D53/02

CPC classification number: C08J9/286 ,  B01D53/02 ,  B01D53/62 ,  B01D2253/202 ,  B01D2253/308 ,  B01D2257/502 ,  B01D2257/504 ,  B01J20/262 ,  B01J20/264 ,  B01J20/28007 ,  B01J20/28057 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/2808 ,  B01J20/28083 ,  B01J20/3028 ,  B82Y30/00 ,  B82Y40/00 ,  C08G12/32 ,  C08G2101/00 ,  C08J9/228 ,  C08J2205/044 ,  C08J2361/28 ,  C08L61/28 ,  C08L61/32 ,  Y02C10/04 ,  Y02C10/08 ,  Y02P20/152 ,  Y10T428/2982

Abstract: The present disclosure relates to a polymer material comprising mesopores extending between melamine-formaldehyde co-polymer nano-particles and wherein micropores extend within the co-polymer nano-particles, methods of producing the same and uses thereof.