公开(公告)号：US09932566B2

公开(公告)日：2018-04-03

申请号：US14820521

申请日：2015-08-06

Applicant: Agilent Technologies, Inc.

Inventor： Andrew Kennedy ,  Daniel E. Ryan

IPC: C12N15/00 ,  C12N9/22 ,  C12N15/90 ,  C12N15/10 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/902 ,  C12N2310/20 ,  C12Y301/04

Abstract: This invention discloses reagents and methods for increasing specificity and efficiency of RNA-guided genome editing.

公开(公告)号：US20170058298A1

公开(公告)日：2017-03-02

申请号：US15251235

申请日：2016-08-30

Applicant: Agilent Technologies, Inc.

Inventor： Andrew Kennedy ,  Gusti Zeiner ,  Subhadeep Roy ,  Chong Wing Yung

IPC: C12N15/90 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/111 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/3519

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

Abstract translation: 本发明涉及包含具有一个或多个修饰的衔接子部分的引导RNA，以及它们在CRISPR：Cas系统的同源重组中的用途。 修饰的衔接片段耐RNaseH降解。 本发明还涉及双指导RNA策略，其中第一引导RNA指导Cas酶在第一靶序列处进行双链断裂，并且第二引导RNA包含连接于供体多核苷酸的衔接子片段，以及 结合与第一靶序列偏移的第二靶序列。

公开(公告)号：US10526590B2

公开(公告)日：2020-01-07

申请号：US15251235

申请日：2016-08-30

Applicant: Agilent Technologies, Inc.

Inventor： Andrew Kennedy ,  Gusti Zeiner ,  Subhadeep Roy ,  Chong Wing Yung

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

公开(公告)号：US20160040189A1

公开(公告)日：2016-02-11

申请号：US14820521

申请日：2015-08-06

Applicant: Agilent Technologies, Inc.

Inventor： Andrew Kennedy ,  Daniel E. Ryan

IPC: C12N15/90 ,  C12N9/22

CPC classification number: C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/902 ,  C12N2310/20 ,  C12Y301/04

Abstract: This invention discloses reagents and methods for increasing specificity and efficiency of RNA-guided genome editing.

Abstract translation: 本发明公开了用于提高RNA指导基因组编辑的特异性和效率的试剂和方法。

公开(公告)号：US10538750B2

公开(公告)日：2020-01-21

申请号：US15442437

申请日：2017-02-24

Applicant: Agilent Technologies, Inc.

Inventor： Chong Wing Yung ,  Andrew Kennedy

IPC: C12N9/22

Abstract: This invention relates to reagents and methods for increasing specificity and efficiency of genome editing by CRISPR associated (Cas) protein systems, more particularly by Cas:guide RNA complexes, by blocking off-target nucleic acids from cleavage by Cas:guide RNA complexes.

公开(公告)号：US20170247671A1

公开(公告)日：2017-08-31

申请号：US15442437

申请日：2017-02-24

Applicant: Agilent Technologies, Inc.

Inventor： Chong Wing Yung ,  Andrew Kennedy

IPC: C12N9/22

Abstract: This invention relates to reagents and methods for increasing specificity and efficiency of genome editing by CRISPR associated (Cas) protein systems, more particularly by Cas:guide RNA complexes, by blocking off-target nucleic acids from cleavage by Cas:guide RNA complexes.