公开(公告)号：US20150148239A1

公开(公告)日：2015-05-28

申请号：US14493127

申请日：2014-09-22

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  Robert A. Ach ,  Alicia Scheffer-Wong ,  Carolina Caffaro

IPC: C12Q1/68

CPC classification number: C12Q1/6841 ,  C12Q2565/513 ,  C12Q2565/514

Abstract: This disclosure provides, among other things, a method for analyzing a planar cellular sample. In some embodiments, the method comprises: (a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the feature in which the oligonucleotides is present; (c) hybridizing the nucleic acid tags, or a copy of the same, with the oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in the duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. Other embodiments, e.g., kits and the like, are also described.

Abstract translation: 本公开尤其提供了一种用于分析平面细胞样品的方法。 在一些实施方案中，所述方法包括：（a）将核酸标签间接或直接附着于平面细胞样品中的结合位点; （b）使所述平面细胞样品与包含寡核苷酸的空间寻址特征阵列的固体支持物接触，其中每个寡核苷酸包含鉴定存在寡核苷酸的特征的分子条形码; （c）将核酸标签或其拷贝与寡核苷酸杂交以产生双链体; 和（d）在双链体中延伸寡核苷酸以产生延伸产物，其各自包含（i）分子条形码和（ii）核酸标签的拷贝。 还描述了其它实施例，例如套件等。

公开(公告)号：US09834814B2

公开(公告)日：2017-12-05

申请号：US14493127

申请日：2014-09-22

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  Robert A. Ach ,  Alicia Scheffer-Wong ,  Carolina Caffaro

IPC: C12Q1/68 ,  G01N33/53 ,  C07H21/00

CPC classification number: C12Q1/6841 ,  C12Q2565/513 ,  C12Q2565/514

Abstract: This disclosure provides, among other things, a method for analyzing a planar cellular sample. In some embodiments, the method comprises: (a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the feature in which the oligonucleotides is present; (c) hybridizing the nucleic acid tags, or a copy of the same, with the oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in the duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. Other embodiments, e.g., kits and the like, are also described.