公开(公告)号：US09771575B2

公开(公告)日：2017-09-26

申请号：US14745174

申请日：2015-06-19

Applicant: Agilent Technologies, Inc.

Inventor： Alexander Belyaev ,  Nicholas M. Sampas ,  Alicia Scheffer-Wong

IPC: C12Q1/68 ,  C12N15/10 ,  C12N9/12 ,  C07H21/04

CPC classification number: C12N15/1065 ,  C12Q2521/327 ,  C12Q2563/179

Abstract: The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.

公开(公告)号：US20150367345A1

公开(公告)日：2015-12-24

申请号：US14310838

申请日：2014-06-20

Applicant: Agilent Technologies, Inc.

Inventor： Chong Wing Yung ,  Alicia Scheffer-Wong

IPC: B01L3/00 ,  B01D21/26 ,  G01N15/10

CPC classification number: B01L3/502753 ,  B01D21/262 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/16 ,  B01L2400/0409 ,  B01L2400/043 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/14 ,  G01N2001/4083 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: A microfluidic disk for concentrating particles includes a plurality of distribution channels and separation channels. A sample fluid is flowed through the distribution channels while the disk is spun. Particles of the sample fluid flow into the separation channels where they accumulate. The particles in the separation channels may be subjected to an analysis.

Abstract translation: 用于浓缩颗粒的微流体盘包括多个分配通道和分离通道。 当盘旋转时，样品流体流过分配通道。 样品流体的颗粒流入它们积聚的分离通道。 可以对分离通道中的颗粒进行分析。

公开(公告)号：US20150148239A1

公开(公告)日：2015-05-28

申请号：US14493127

申请日：2014-09-22

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  Robert A. Ach ,  Alicia Scheffer-Wong ,  Carolina Caffaro

IPC: C12Q1/68

CPC classification number: C12Q1/6841 ,  C12Q2565/513 ,  C12Q2565/514

Abstract: This disclosure provides, among other things, a method for analyzing a planar cellular sample. In some embodiments, the method comprises: (a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the feature in which the oligonucleotides is present; (c) hybridizing the nucleic acid tags, or a copy of the same, with the oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in the duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. Other embodiments, e.g., kits and the like, are also described.

Abstract translation: 本公开尤其提供了一种用于分析平面细胞样品的方法。 在一些实施方案中，所述方法包括：（a）将核酸标签间接或直接附着于平面细胞样品中的结合位点; （b）使所述平面细胞样品与包含寡核苷酸的空间寻址特征阵列的固体支持物接触，其中每个寡核苷酸包含鉴定存在寡核苷酸的特征的分子条形码; （c）将核酸标签或其拷贝与寡核苷酸杂交以产生双链体; 和（d）在双链体中延伸寡核苷酸以产生延伸产物，其各自包含（i）分子条形码和（ii）核酸标签的拷贝。 还描述了其它实施例，例如套件等。

公开(公告)号：US09834814B2

公开(公告)日：2017-12-05

申请号：US14493127

申请日：2014-09-22

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  Robert A. Ach ,  Alicia Scheffer-Wong ,  Carolina Caffaro

IPC: C12Q1/68 ,  G01N33/53 ,  C07H21/00

CPC classification number: C12Q1/6841 ,  C12Q2565/513 ,  C12Q2565/514

Abstract: This disclosure provides, among other things, a method for analyzing a planar cellular sample. In some embodiments, the method comprises: (a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the feature in which the oligonucleotides is present; (c) hybridizing the nucleic acid tags, or a copy of the same, with the oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in the duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. Other embodiments, e.g., kits and the like, are also described.

公开(公告)号：US20170175182A1

公开(公告)日：2017-06-22

申请号：US15290622

申请日：2016-10-11

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Brian Jon Peter ,  Robert A. Ach ,  Alicia Scheffer-Wong

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2521/507 ,  C12Q2565/514

Abstract: Provided herein, among other things, are a variety of methods that comprise inserting a plurality of barcoded transposons into a population of DNA fragments that comprise DNA fragments of less than 1 kb in length, to produce transposon-tagged fragments that each comprise a barcoded transposon. Kits for performing this method are also provided.

公开(公告)号：US20160369266A1

公开(公告)日：2016-12-22

申请号：US14745174

申请日：2015-06-19

Applicant: Agilent Technologies, Inc.

Inventor： Alexander Belyaev ,  Nicholas M. Sampas ,  Alicia Scheffer-Wong

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12Q2521/327 ,  C12Q2563/179

Abstract: The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.

Abstract translation: 本公开提供了使用含条形码的转座酶复合物的微阵列条形码编码多个DNA样品的方法。 在一些实施方案中，DNA样品和转座酶复合物存在于相对底物表面上的水性液滴中，这允许单个DNA样品液滴与单个转座酶复合物液滴组合。 组合液滴中的条形码DNA可用于任何数量的目的，包括用于扩增和测序的模板。