公开(公告)号：US20240018510A1

公开(公告)日：2024-01-18

申请号：US18256877

申请日：2020-12-10

Applicant: Agilent Technologies, Inc.

Inventor： David Taussig ,  Israel Steinfeld ,  Nicholas M Sampas ,  Brian Jon Peter

IPC: C12N15/10 ,  C12Q1/6869

CPC classification number: C12N15/1065 ,  C12Q1/6869

Abstract: The present invention relates to preparation, sequencing and analysis of a sequencing library of adaptor-tagged fragments, wherein the fragments have different orientations relative to a sequencing adaptor.

公开(公告)号：US20180201924A1

公开(公告)日：2018-07-19

申请号：US15409124

申请日：2017-01-18

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  David Taussig ,  Bahram Arezi ,  Robert A. Ach ,  Nicholas M. Sampas

IPC: C12N15/10 ,  C12Q1/68

Abstract: A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a prior of tailed primers to produce asymmetrically-tagged library.

公开(公告)号：US10711269B2

公开(公告)日：2020-07-14

申请号：US15409124

申请日：2017-01-18

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  David Taussig ,  Bahram Arezi ,  Robert A. Ach ,  Nicholas M. Sampas

IPC: C12N15/10 ,  C12Q1/6806

Abstract: A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a pair of tailed primers to produce asymmetrically-tagged library.