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    HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS
    4.
    发明申请
    HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS 审中-公开
    高渗透基因组装在DROPLETS

    公开(公告)号：US20150361423A1

    公开(公告)日：2015-12-17

    申请号：US14486346

    申请日：2014-09-15

    Applicant: Agilent Technologies, Inc.

    Inventor： Jeffrey R. Sampson ,  Nicholas M. Sampas

    IPC: C12N15/10

    CPC classification number: C12N15/1093 ,  C12N15/1075

    Abstract: Provided herein is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal indexer sequence and can be assembled to produce a synthon; and (b) hybridizing the oligonucleotide mixture to an array, thereby spatially-separating the different sets of oligonucleotides from one another. In some embodiments the method may comprise (c) contacting the array with a solution, thereby producing, for each feature bound by the oligonucleotides, a discrete droplet comprising the feature and, optionally, placing an immiscible liquid over the droplets, thereby producing, for each feature bound by the oligonucleotides, a discrete reaction chamber defined by a droplet. The method may further comprise incubating the array under conditions by which a synthon is assembled in each of the reaction chambers. Other embodiments are also provided.

    Abstract translation: 本文提供的方法包括：（a）获得多组寡核苷酸的混合物，其中每组中的寡核苷酸各自包含末端分子序列，并且可以组装以产生合成子; 和（b）将寡核苷酸混合物与阵列杂交，从而将不同组的寡核苷酸彼此空间分离。 在一些实施方案中，该方法可以包括（c）使阵列与溶液接触，从而为由寡核苷酸结合的每个特征产生包含特征的离散液滴，并且任选地将不混溶液体放置在液滴上，从而产生 由寡核苷酸结合的每个特征，由液滴限定的离散反应室。 该方法还可以包括在每个反应室中组合合成子的条件下孵育该阵列。 还提供了其他实施例。

    Nucleic Acid Proximity Assay Involving the Formation of a Three-way junction
    6.
    发明申请
    Nucleic Acid Proximity Assay Involving the Formation of a Three-way junction 审中-公开
    涉及形成三向连接点的核酸接近度测定

    公开(公告)号：US20140212869A1

    公开(公告)日：2014-07-31

    申请号：US13750810

    申请日：2013-01-25

    Applicant: AGILENT TECHNOLOGIES, INC.

    Inventor： Nicholas M. Sampas ,  Robert A. Ach ,  Nazumi Alice Yamada ,  Kristin Bernick

    IPC: C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q2522/101 ,  C12Q2525/161 ,  C12Q2565/101 ,  C12Q2525/301 ,  C12Q2561/125

    Abstract: Provided herein is a proximity assay that, in certain embodiments, involves: (a) hybridizing a first oligonucleotide and a second oligonucleotide with a target nucleic acid, wherein the first oligonucleotide comprises: i. a region that is complementary to a first sequence in the target nucleic acid and ii. a barcode sequence; and the second oligonucleotide comprises i. a region that is complementary to a second region in the target and ii. the complement of the barcode sequence; and (b) detecting hybridization between the barcode sequence and the complement of the barcode sequence, wherein hybridization between the barcode sequence and the complement of the barcode sequence indicates that the first and second target sequences are proximal to one another in the sample.

    Abstract translation: 本文提供了在某些实施方案中的邻近测定法，其包括：（a）将第一寡核苷酸和第二寡核苷酸与靶核酸杂交，其中所述第一寡核苷酸包含： 与靶核酸中的第一序列互补的区域，和ii。 条形码序列; 并且第二寡核苷酸包括i。 与目标中的第二区域互补的区域，以及ii。 条形码序列的补码; 和（b）检测条形码序列与条形码序列的互补之间的杂交，其中条形码序列与条形码序列的互补之间的杂交表明第一和第二靶序列在样本中彼此靠近。

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