公开(公告)号：US20240018510A1

公开(公告)日：2024-01-18

申请号：US18256877

申请日：2020-12-10

Applicant: Agilent Technologies, Inc.

Inventor： David Taussig ,  Israel Steinfeld ,  Nicholas M Sampas ,  Brian Jon Peter

IPC: C12N15/10 ,  C12Q1/6869

CPC classification number: C12N15/1065 ,  C12Q1/6869

Abstract: The present invention relates to preparation, sequencing and analysis of a sequencing library of adaptor-tagged fragments, wherein the fragments have different orientations relative to a sequencing adaptor.

公开(公告)号：US10684199B2

公开(公告)日：2020-06-16

申请号：US15995755

申请日：2018-06-01

Applicant: Agilent Technologies, Inc.

Inventor： Kyle Schleifer ,  Kristin Briana Bernick ,  Adrienne Mccampbell ,  Nicholas M Sampas ,  Victor Lim

IPC: G01N1/30 ,  G01N1/06 ,  G01N1/28 ,  G01N35/00 ,  G06T7/00

Abstract: The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.

公开(公告)号：US10538796B2

公开(公告)日：2020-01-21

申请号：US15782648

申请日：2017-10-12

Applicant: Agilent Technologies, Inc.

Inventor： Nicholas M Sampas ,  Jeffrey R Sampson

IPC: C12P19/34 ,  C12N15/10 ,  C12N15/66

Abstract: Provided herein, among other things, is a method for producing a ligation product on a support. In some embodiments, the method may comprise hybridizing a first double-stranded oligonucleotides and a second double-stranded oligonucleotide to a substrate comprising surface-tethered oligonucleotides and ligating the distal ends of the first and second double-stranded oligonucleotides together, thereby producing a first ligation product that is tethered to the support at both ends.

公开(公告)号：US20180305683A1

公开(公告)日：2018-10-25

申请号：US15938306

申请日：2018-03-28

Applicant: Agilent Technologies, Inc.

Inventor： Nicholas M Sampas

IPC: C12N15/10 ,  C12Q1/6869 ,  C12N9/96 ,  C12N9/12

CPC classification number: C12N15/1065 ,  C12N9/1241 ,  C12N9/96 ,  C12Q1/6869

Abstract: Described herein, among other things, is a method for amplifying a nucleic acid sample. In some embodiments this method may comprise (a) tagmenting the nucleic acid sample with a population of transposase complexes, wherein the population of transposase complexes comprise: i. a transposase and ii. a set of adaptors of the formula X-Y, wherein: region X is a variable sequence that has a complexity of n, wherein n is at least 3, and region Y is a recognition sequence for the transposase, and (b) amplifying the tagged fragments using a set of primers, wherein the set of primers comprises at least n different primers and the n different primers collectively hybridize to all of the different sequences of region X, or a complement thereof.

公开(公告)号：US20180105853A1

公开(公告)日：2018-04-19

申请号：US15782648

申请日：2017-10-12

Applicant: Agilent Technologies, Inc.

Inventor： Nicholas M Sampas ,  Jeffrey R Sampson

IPC: C12P19/34 ,  C12N15/66 ,  C12N15/10

CPC classification number: C12P19/34 ,  C12N15/1006 ,  C12N15/1031 ,  C12N15/66 ,  C12Q1/6837

Abstract: Provided herein, among other things, is a method for producing a ligation product on a support. In some embodiments, the method may comprise hybridizing a first double-stranded oligonucleotides and a second double-stranded oligonucleotide to a substrate comprising surface-tethered oligonucleotides and ligating the distal ends of the first and second double-stranded oligonucleotides together, thereby producing a first ligation product that is tethered to the support at both ends.