公开(公告)号：US20140255924A1

公开(公告)日：2014-09-11

申请号：US13791616

申请日：2013-03-08

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： NICHOLAS M. SAMPAS ,  Kristin Bernick

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q1/6818 ,  C12Q2525/161 ,  C12Q2565/101

Abstract: A composition comprising population of labeled oligonucleotides is provided herein. In some embodiments, the probes are of the formula: T1-V-T2, wherein: the nucleotide sequence of the V region varies in said population; the V regions of the different oligonucleotides hybridize to sites that are tiled across a sequence in a target nucleic acid; the T1 and T2 regions do not hybridize with said target nucleic acid; within each oligonucleotide of the population, the T1 and T2 regions are not complementary; and within each oligonucleotide of the population, the T1 region is complementary to the T2 region of at least one of other oligonucleotide of the population. Also provided is a method that comprises hybridizing the population of labeled oligonucleotides with a target nucleic acid to produce a complex.

Abstract translation: 提供包含标记寡核苷酸群的组合物。 在一些实施方案中，探针具有下式：T1-V-T2，其中：V区的核苷酸序列在所述群体中变化; 不同寡核苷酸的V区与靶核酸中的序列平铺的位点杂交; T1和T2区不与所述靶核酸杂交; 在群体的每个寡核苷酸内，T1和T2区域不互补; 并且在群体的每个寡核苷酸内，T1区域与群体的其他寡核苷酸中的至少一个的T2区域互补。 还提供了一种方法，其包括将标记的寡核苷酸群与靶核酸杂交以产生复合物。

公开(公告)号：US20140212869A1

公开(公告)日：2014-07-31

申请号：US13750810

申请日：2013-01-25

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Nicholas M. Sampas ,  Robert A. Ach ,  Nazumi Alice Yamada ,  Kristin Bernick

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q2522/101 ,  C12Q2525/161 ,  C12Q2565/101 ,  C12Q2525/301 ,  C12Q2561/125

Abstract: Provided herein is a proximity assay that, in certain embodiments, involves: (a) hybridizing a first oligonucleotide and a second oligonucleotide with a target nucleic acid, wherein the first oligonucleotide comprises: i. a region that is complementary to a first sequence in the target nucleic acid and ii. a barcode sequence; and the second oligonucleotide comprises i. a region that is complementary to a second region in the target and ii. the complement of the barcode sequence; and (b) detecting hybridization between the barcode sequence and the complement of the barcode sequence, wherein hybridization between the barcode sequence and the complement of the barcode sequence indicates that the first and second target sequences are proximal to one another in the sample.

Abstract translation: 本文提供了在某些实施方案中的邻近测定法，其包括：（a）将第一寡核苷酸和第二寡核苷酸与靶核酸杂交，其中所述第一寡核苷酸包含： 与靶核酸中的第一序列互补的区域，和ii。 条形码序列; 并且第二寡核苷酸包括i。 与目标中的第二区域互补的区域，以及ii。 条形码序列的补码; 和（b）检测条形码序列与条形码序列的互补之间的杂交，其中条形码序列与条形码序列的互补之间的杂交表明第一和第二靶序列在样本中彼此靠近。

公开(公告)号：US09890417B2

公开(公告)日：2018-02-13

申请号：US14531563

申请日：2014-11-03

Applicant: Agilent Technologies, Inc.

Inventor： Kristin Bernick ,  Robert Ach ,  Mistuni Ghosh ,  Brian Smart

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q2563/125 ,  C12Q2563/131

Abstract: Among other things, this disclosure provides a method of detecting a target nucleic acid. Aspects of the method include: (a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe comprises a capture tag; (b) hybridizing the probe with the target nucleic in a fixed cell, in situ, to produce a duplex; (c) linking the probe in the duplex to a peroxidase conjugate via the capture tag to produce a peroxidase-labeled duplex; and (d) incubating the peroxidase-labeled duplex with a peroxidase substrate, wherein the peroxidase activity of the peroxidase conjugate catalyzes deposition of the substrate in the vicinity of the duplex, thereby producing a detectable signal.

公开(公告)号：US20160122800A1

公开(公告)日：2016-05-05

申请号：US14531563

申请日：2014-11-03

Applicant: Agilent Technologies, Inc.

Inventor： Kristin Bernick ,  Robert Ach ,  Mistuni Ghosh ,  Brian Smart

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q2563/125 ,  C12Q2563/131

Abstract: Among other things, this disclosure provides a method of detecting a target nucleic acid. Aspects of the method include: (a) obtaining a labeled nucleic acid probe that is complementary to a target nucleic acid, wherein the probe comprises a capture tag; (b) hybridizing the probe with the target nucleic in a fixed cell, in situ, to produce a duplex; (c) linking the probe in the duplex to a peroxidase conjugate via the capture tag to produce a peroxidase-labeled duplex; and (d) incubating the peroxidase-labeled duplex with a peroxidase substrate, wherein the peroxidase activity of the peroxidase conjugate catalyzes deposition of the substrate in the vicinity of the duplex, thereby producing a detectable signal.

Abstract translation: 除此之外，本公开提供了检测靶核酸的方法。 该方法的方面包括：（a）获得与靶核酸互补的标记核酸探针，其中所述探针包含捕获标签; （b）将位于固定细胞中的探针与靶核酸原位杂交以产生双链体; （c）通过捕获标签将双链体中的探针与过氧化物酶缀合物连接以产生过氧化物酶标记的双链体; 和（d）过氧化物酶标记的双链体与过氧化物酶底物孵育，其中过氧化物酶缀合物的过氧化物酶活性催化底物在双相体附近的沉积，从而产生可检测的信号。