公开(公告)号：US11746390B2

公开(公告)日：2023-09-05

申请号：US16922829

申请日：2020-07-07

申请人： Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences ,  AGRICULTURAL GENOMICS INSTITUTE AT SHENZHEN, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Sanwen Huang ,  Chunzhi Zhang ,  Zhongmin Yang ,  Die Tang

IPC分类号： C12Q1/6895 ,  A01H1/02 ,  A01H6/82 ,  A01H1/04

CPC分类号： C12Q1/6895 ,  A01H1/02 ,  A01H1/022 ,  A01H1/045 ,  A01H6/827 ,  C12Q2600/13 ,  C12Q2600/158

摘要： Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as Ss11 and Ss12 respectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of Ss11Ss11 from the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible F1 generation by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.

公开(公告)号：US12000005B2

公开(公告)日：2024-06-04

申请号：US17619970

申请日：2021-12-16

申请人： Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences ,  Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

发明人： Jianbin Yan ,  Qinggang Liao ,  Junbo Gou ,  Ran Du ,  Sanwen Huang

IPC分类号： C12Q1/68 ,  C12Q1/6895 ,  C12Q1/6844

CPC分类号： C12Q1/6895 ,  C12Q1/6844 ,  C12Q2600/13

摘要： Provided is primers, where the primers specifically bind to a polynucleotide and are used to amplify the polynucleotide; and the polynucleotide comprises or consists of the following sequence: 1) the nucleotide sequence represented by SEQ ID NO: 5; or 2) the complete complementary sequence of the sequence represented by SEQ ID NO: 5; or 3) a nucleotide sequence being identical to 300 bp or more contiguous nucleotides in the sequence of the above 1) or 2), where the nucleotide sequence being identical to 300 bp or more contiguous nucleotides is unique to a male Ginkgo biloba plant; and the sequences of the primers are respectively represented by SEQ ID NO: 1 and SEQ ID NO: 2.

公开(公告)号：US12104190B2

公开(公告)日：2024-10-01

申请号：US18147862

申请日：2022-12-29

申请人： AGRICULTURAL GENOMICS INSTITUTE AT SHENZHEN, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Jianbin Yan ,  Junbo Gou ,  Qinggang Liao ,  Haifei Liang ,  Chong Li ,  Ran Du ,  Xingyao Xiong ,  Sanwen Huang

IPC分类号： C12N9/88 ,  C12N15/52 ,  C12N15/82

CPC分类号： C12N9/88 ,  C12N15/52 ,  C12N15/8243 ,  C12Y402/03017

摘要： Provided are a taxadiene synthase TcTS2, an encoding nucleotide sequence and use thereof. The amino acid sequence of TcTS2 includes or consists of: (a) an amino acid sequence represented by SEQ ID NO: 1; or (b) a functional homologous sequence having at least 80% sequence similarity with the amino acid sequence represented by SEQ ID NO: 1; or (c) an amino acid sequence having TcTS2 activity with addition, deletion, or substitution of one or more amino acids in the amino acid sequence represented by SEQ ID NO: 1. TcTS2 and the nucleotide sequence encoding the TcTS2 provide new gene resources for improving the yield of taxol, and they may be used for modifying chassis hosts by plant genetic engineering and metabolic engineering strategies to produce taxol and the intermediates thereof etc., thereby having significant economic and social value.