公开(公告)号：US20130171700A1

公开(公告)日：2013-07-04

申请号：US13772959

申请日：2013-02-21

Applicant: Ajinomoto Co., Inc.

Inventor： Yoshiya Gunji ,  Hisashi Yasueda ,  Shinichi Sugimoto ,  Nobuharu Tsujimoto ,  Megumi Shimaoka ,  Yuri Miyata ,  Manami Oba

IPC: C12P13/08

CPC classification number: C12P13/08 ,  C12N9/1217 ,  C12N9/88 ,  C12P13/04 ,  C12R1/01

Abstract: An L-amino acid is produced by culturing a Methylophilus bacterium which can grow by using methanol as the main carbon source and has L-amino acid-producing ability, for example, a Methylophilus bacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation of cells with a DNA coding for dihydrodipicolinate synthase that is desensitized to feedback inhibition by L-lysine and a DNA coding for aspartokinase that is desensitized to feedback inhibition by L-lysine, or a Methylophilus bacterium which is casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.

Abstract translation: 通过培养可以通过使用甲醇作为主要碳源生长并且具有L-氨基酸生产能力的甲型幽门螺杆菌细菌产生L-氨基酸，例如其中二氢吡啶二酸合成酶活性和天冬氨酸激酶活性被增强的甲基幽门螺杆菌 将编码二脱氧叶酸二磷酸合酶的DNA转化为L-赖氨酸的反馈抑制的脱氧赖氨酸的编码天冬氨酸激酶的DNA或L-赖氨酸反馈抑制的天冬氨酸的DNA或作为酪蛋白氨基酸营养缺陷型的细鳞状芽孢杆菌属细菌的DNA在培养基 含有甲醇作为主要碳源，在培养中产生和积累L-氨基酸，并从培养物中收集L-氨基酸。