公开(公告)号：US20060063252A1

公开(公告)日：2006-03-23

申请号：US11226247

申请日：2005-09-15

申请人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata ,  Hiroko Terasaki

发明人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata ,  Hiroko Terasaki

IPC分类号： C12N5/06

CPC分类号： C12N5/0629 ,  C12M25/00 ,  C12N5/0075 ,  C12N11/14 ,  C12N13/00

摘要： Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium magnet was placed outside the bottom of the well. As a result, control epidermal keratinocytes not incorporating magnetic particles therein did not adhere to the bottom of the well and did not form an undifferentiated cell sheet. On the contrary, epidermal keratinocytes incorporating magnetic particles therein, while being attracted to the bottom of the well by magnetic force, formed a multilayered cell sheet. This cell sheet could be easily recovered by removing the neodymium magnet placed outside the bottom of the well, then bringing a magnet having a hydrophilic film attached thereto close to the cell sheet from upside and to attract it via this hydrophilic film, and lifting it.

摘要翻译： 将不含有磁性颗粒的表皮角化细胞和其中含有磁性颗粒的表皮角质形成细胞接种在24孔超低附着板中，并在将钕磁铁放置在孔底部之外培养1天。 结果，不含有磁性颗粒的对照表皮角化细胞不粘附到孔的底部，并且不形成未分化的细胞片。 相反，在其中通过磁力吸引到井底部的同时包含磁性颗粒的表皮角质形成细胞形成多层细胞片。 通过去除放置在孔底部的钕磁铁，可以容易地回收该细胞片，然后将从其上方附着有亲水膜的磁体附着到细胞片上，并通过该亲水性膜吸引，并将其提起。