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    Cell Culture Method and Automatic Culture System Using the Method
    1.
    发明申请
    Cell Culture Method and Automatic Culture System Using the Method 审中-公开
    细胞培养方法及自动培养系统

    公开(公告)号：US20100317102A1

    公开(公告)日：2010-12-16

    申请号：US12160613

    申请日：2006-11-14

    申请人： Tsutomu Suzuki ,  Daisuke Iejima ,  Hideaki Kagami ,  Ryuji Kato ,  Minoru Ueda ,  Kunihiko Okada

    发明人： Tsutomu Suzuki ,  Daisuke Iejima ,  Hideaki Kagami ,  Ryuji Kato ,  Minoru Ueda ,  Kunihiko Okada

    IPC分类号： C12N5/071 ,  C12M1/00

    CPC分类号： C12N5/0602 ,  C12M23/00 ,  C12M23/14 ,  C12M29/06 ,  C12M33/04 ,  C12M41/14 ,  C12M41/36 ,  C12M41/48

    摘要： A culturing method according to the present invention including a step of injecting cells extracted from a human body and a culture medium into a culture container, (2) a step of culturing cells while performing air ventilation and exchange of the culture medium in the culture container in which the culture medium and the cells are put, (3) a step of cleaning cultured cells which are cultured in the culture container and then withdrawing the cleaned cells from the culture container into a cell withdrawing vessel while some of the cleaned cells are left in the culture container, (4) a step of injecting a culture medium to the cultured cells left in the culture container in the step (3), and (5) a step of repetitively executing the step (2,) and the step (3).

    摘要翻译： 根据本发明的培养方法，包括将从人体提取的细胞和培养基注入培养容器的步骤，（2）在进行空气通风并在培养容器中更换培养基的步骤 其中放置培养基和细胞，（3）清洗在培养容器中培养的培养细胞，然后将清洁的细胞从培养容器中取出到细胞抽出容器中，同时将一些清洁的细胞留下 在培养容器中，（4）在步骤（3）中向培养容器中留下的培养细胞注入培养基的步骤，（5）重复执行步骤（2）和步骤（ 3）。

    Cell culture method and cultured tissue
    3.
    发明申请
    Cell culture method and cultured tissue 审中-公开
    细胞培养法和培养组织

    公开(公告)号：US20060121606A1

    公开(公告)日：2006-06-08

    申请号：US11271911

    申请日：2005-11-14

    申请人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata

    发明人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0062

    摘要： A cell culture method prepares first cells that are adhesion-dependent cells and monolayered or multilayered cells on a culture surface of a culture substrate, seeds second cells that are adhesion-dependent cells and are magnetized by allowing to have magnetic particles on the first cells, induces the second cells to a predetermined position on the first cells by magnetic force, and cultures the first cells and the second cells in a cell arrangement obtained by the magnetic induction. According to this cell culture method, after a cell sheet was prepared individually, cells can be multilayered without changing a temperature, peeling the monolayered sheet and laminating the monolayered sheets.

    摘要翻译： 细胞培养方法在培养基底的培养表面上制备作为粘附依赖性细胞和单层或多层细胞的第一细胞，作为粘附依赖性细胞的种子第二细胞并通过在第一细胞上具有磁性颗粒而被磁化， 通过磁力将第二细胞诱导到第一细胞上的预定位置，并以通过磁感应获得的细胞排列来培养第一细胞和第二细胞。 根据该细胞培养方法，单独制备细胞片后，细胞可以多层而不改变温度，剥离单层片材并层压单层片材。

    Material for ameliorating skin tissue and method for producing the same
    4.
    发明授权
    Material for ameliorating skin tissue and method for producing the same 有权
    用于改善皮肤组织的材料及其制备方法

    公开(公告)号：US08338174B2

    公开(公告)日：2012-12-25

    申请号：US12490764

    申请日：2009-06-24

    申请人： Katsumi Ebisawa ,  Ryuji Kato ,  Hideaki Kagami ,  Minoru Ueda

    发明人： Katsumi Ebisawa ,  Ryuji Kato ,  Hideaki Kagami ,  Minoru Ueda

    IPC分类号： C12N5/07

    CPC分类号： C12N5/0629 ,  A61K35/12 ,  A61L27/3804 ,  A61L27/3839 ,  C12N2533/92

    摘要： A material for ameliorating skin tissue provided by the invention comprises, as a main component, a culture obtained by culturing cells or tissue fragments derived from human or other mammalian alveolar mucosa. Typically, 50% or more of the cells contained in the culture are fibroblasts, and having a high growth rate and a high productivity in vascular endothelial cell growth factor (VEDF) and/or keratinocyte growth factor (KGF).

    摘要翻译： 本发明提供的用于改善皮肤组织的材料包含通过培养源自人或其他哺乳动物肺泡粘膜的细胞或组织片段获得的培养物作为主要成分。 通常，培养物中含有50％以上的细胞是成纤维细胞，并且在血管内皮细胞生长因子（VEDF）和/或角质形成细胞生长因子（KGF）中具有高生长速度和高生产率。

    Cell culture method and cell sheet
    5.
    发明申请
    Cell culture method and cell sheet 审中-公开
    细胞培养方法和细胞片

    公开(公告)号：US20060063252A1

    公开(公告)日：2006-03-23

    申请号：US11226247

    申请日：2005-09-15

    申请人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata ,  Hiroko Terasaki

    发明人： Akira Ito ,  Hiroyuki Honda ,  Takeshi Kobayashi ,  Minoru Ueda ,  Hideaki Kagami ,  Ken-ichiro Hata ,  Hiroko Terasaki

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0629 ,  C12M25/00 ,  C12N5/0075 ,  C12N11/14 ,  C12N13/00

    摘要： Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium magnet was placed outside the bottom of the well. As a result, control epidermal keratinocytes not incorporating magnetic particles therein did not adhere to the bottom of the well and did not form an undifferentiated cell sheet. On the contrary, epidermal keratinocytes incorporating magnetic particles therein, while being attracted to the bottom of the well by magnetic force, formed a multilayered cell sheet. This cell sheet could be easily recovered by removing the neodymium magnet placed outside the bottom of the well, then bringing a magnet having a hydrophilic film attached thereto close to the cell sheet from upside and to attract it via this hydrophilic film, and lifting it.

    摘要翻译： 将不含有磁性颗粒的表皮角化细胞和其中含有磁性颗粒的表皮角质形成细胞接种在24孔超低附着板中，并在将钕磁铁放置在孔底部之外培养1天。 结果，不含有磁性颗粒的对照表皮角化细胞不粘附到孔的底部，并且不形成未分化的细胞片。 相反，在其中通过磁力吸引到井底部的同时包含磁性颗粒的表皮角质形成细胞形成多层细胞片。 通过去除放置在孔底部的钕磁铁，可以容易地回收该细胞片，然后将从其上方附着有亲水膜的磁体附着到细胞片上，并通过该亲水性膜吸引，并将其提起。

    Method of regenerating tooth germ and a regenerated tooth germ
    7.
    发明申请
    Method of regenerating tooth germ and a regenerated tooth germ 审中-公开
    再生牙胚和再生牙胚的方法

    公开(公告)号：US20060177386A1

    公开(公告)日：2006-08-10

    申请号：US10516108

    申请日：2003-05-30

    申请人： Minoru Ueda ,  Masaki Honda ,  Hideaki Kagami ,  Hiromichi Shimizu

    发明人： Minoru Ueda ,  Masaki Honda ,  Hideaki Kagami ,  Hiromichi Shimizu

    IPC分类号： A61K8/96 ,  C12N5/08

    CPC分类号： A61L27/3804 ,  A61L27/3865 ,  A61L27/3895 ,  A61L2430/12

    摘要： An object of the present invention is to provide a method for regenerating tooth germ, and more specifically, to provide a method for regenerating tooth germ that enables the treatment of patients who have lost teeth or have had teeth damaged by dental diseases such as pyorrhea alveolaris or dental caries. The present invention provides a method for regenerating tooth germ by culturing tooth germ cells while giving mechanical stimulus to the cells, and a regenerated tooth germ.

    摘要翻译： 本发明的目的是提供一种再生牙胚的方法，更具体地，提供一种再生牙胚的方法，所述方法能够治疗患有牙齿缺失或牙齿损伤的牙齿疾病如脓疮性牙槽炎 或龋齿。 本发明提供一种通过在对细胞施加机械刺激的同时培养牙胚细胞和再生的牙胚来再生牙胚的方法。