公开(公告)号：US11963695B2

公开(公告)日：2024-04-23

申请号：US17236130

申请日：2021-04-21

申请人： TissueMill Technologies LLC

发明人： Thomas Andrew Davenport ,  Paul Mulhauser ,  Gregory C. Guinan

IPC分类号： A61B17/322 ,  A61L27/36 ,  C12M1/00 ,  C12M1/33 ,  C12N5/071

CPC分类号： A61B17/322 ,  A61L27/362 ,  A61L27/3695 ,  C12M45/02 ,  C12M45/04 ,  C12N5/0629 ,  A61B2017/3225

摘要： A process and system provides for atraumatic preparation of morselized Tissue Particles (TP)s, such as Full Thickness Skin Graft Particles (FTSGPs), cartilage particles and other organ tissue particles, in a liquid medium. The resultant tissue product may be a suspension of Tissue Particles in an aqueous solution and containing highly viable cells and may be rapidly prepared at bedside or in the operating room and conveniently delivered to a patient through a syringe or similar applicator. The morselized Tissues Particles may be used for surgical applications including wound healing, cosmetic surgery, and orthopedic cartilage repairs.

公开(公告)号：US20240002790A1

公开(公告)日：2024-01-04

申请号：US18039033

申请日：2021-11-23

申请人： XCELL THERAPEUTICS ING.

发明人： Ui il LEE ,  Joo youn LEE ,  Hong seok KANG ,  Ji hye LEE ,  Jeong eon Lee ,  Min hee KANG

IPC分类号： C12N5/071

CPC分类号： C12N5/0629 ,  C12N2501/11 ,  C12N2500/14

摘要： The present invention relates to a culture medium composition for culturing keratinocytes, and more specifically, to a culture medium composition for establishing or culturing keratinocytes, comprising calcium, an epidermal growth factor, and albumin According to the present invention, when keratinocytes are cultured using the culture medium of the present invention, the keratinocytes can be effectively proliferated within a short time, thus making it possible to more quickly obtain a desired number of the cells during the development of a therapeutic agent using the keratinocytes, and the activity of the keratinocytes is improved, and thus the culture medium of the present invention can be useful in the fields of keratinocyte research and the development of therapeutic agents.

公开(公告)号：US20190219564A1

公开(公告)日：2019-07-18

申请号：US16302792

申请日：2017-05-22

申请人： The University of Dundee

发明人： Robyn Patricia Hickerson ,  Michael John Conneely

IPC分类号： G01N33/50 ,  C12Q1/6876 ,  C12N5/071 ,  G01N1/36

CPC分类号： G01N33/5005 ,  A01N1/0242 ,  A01N1/0263 ,  A01N1/0278 ,  C12M21/08 ,  C12M23/46 ,  C12M35/04 ,  C12N5/0629 ,  C12Q1/6876 ,  G01N1/36

摘要： A method for improving the ex vivo viability of a skin tissue type by mounting samples of the skin tissue type in the skin sample holder, applying separate tensions to at least some of the samples and measuring the ability of at least some of the skin tissue samples to maintain live skin characteristics, such that, for the skin tissue type, the measurements of live skin characteristics determine one or more value of skin tension which improve the ex vivo viability of the skin sample type. The viability may be determined by measuring keratin 17 expression by a wounded skin sample.

公开(公告)号：US20180320132A1

公开(公告)日：2018-11-08

申请号：US16029892

申请日：2018-07-09

申请人： THE GENERAL HOSPITAL CORPORATION

发明人： Anna I. Mandinova ,  Sam Lee ,  Victor A. Neel

IPC分类号： C12N5/071 ,  G01N33/50 ,  A61K31/713 ,  A61K31/4745 ,  A61K31/353 ,  A61K31/352 ,  C07K16/40

CPC分类号： C12N5/0629 ,  A61K31/352 ,  A61K31/353 ,  A61K31/437 ,  A61K31/4439 ,  A61K31/4745 ,  A61K31/475 ,  A61K31/496 ,  A61K31/7076 ,  A61K31/713 ,  A61K45/06 ,  A61K2039/505 ,  C07K16/40 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2501/734 ,  C12Y207/11001 ,  C12Y304/21004 ,  C12Y304/24004 ,  G01N33/5044 ,  G01N2333/912 ,  G01N2500/10 ,  G01N2510/00

摘要： Provided herein are methods and assays for isolating and culturing seborrheic keratosis cells ex vivo. Also provided herein are screening assays using cultured seborrheic keratosis cells and methods for treating seborrheic keratosis in a subject.

公开(公告)号：US20180230436A1

公开(公告)日：2018-08-16

申请号：US15525241

申请日：2015-10-08

申请人： Garrett VYGANTAS ,  Bibhash MUKHOPADHYAY ,  Darryl CARTER

发明人： Garrett VYGANTAS ,  Bibhash MUKHOPADHYAY ,  Darryl CARTER

IPC分类号： C12N5/074 ,  C12N5/071 ,  C12P21/02

CPC分类号： C12N5/0696 ,  C07K14/4741 ,  C12N5/0629 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2740/10041 ,  C12P21/02

摘要： Methods and compositions are provided for producing synthetically-derived keratin compositions and keratin products.

公开(公告)号：US20170055561A1

公开(公告)日：2017-03-02

申请号：US15284223

申请日：2016-10-03

申请人： Allergan, Inc.

发明人： Gail K. Naughton ,  David L. Horwitz ,  Mark Applegate ,  Joan Zeltinger ,  Jonathan Mansbridge ,  Andreas Kern ,  Lee K. Landeen ,  Anthony Ratcliffe ,  R. Emmett Pinney

IPC分类号： A23L33/10 ,  C12N5/0735 ,  C12N5/071 ,  A61Q19/08 ,  A61K9/00 ,  A61K38/39 ,  A61K8/98 ,  A61L31/04 ,  A61L31/16 ,  A61Q19/00 ,  A61K35/33 ,  A61K35/36 ,  A61K35/50 ,  A61K38/18 ,  C12N5/077

CPC分类号： A23L33/10 ,  A23V2002/00 ,  A61K8/02 ,  A61K8/0208 ,  A61K8/64 ,  A61K8/65 ,  A61K8/985 ,  A61K9/0014 ,  A61K35/33 ,  A61K35/35 ,  A61K35/36 ,  A61K35/50 ,  A61K38/18 ,  A61K38/1825 ,  A61K38/39 ,  A61K45/06 ,  A61K2800/86 ,  A61K2800/91 ,  A61L31/044 ,  A61L31/047 ,  A61L31/16 ,  A61L2300/414 ,  A61L2430/36 ,  A61Q7/00 ,  A61Q19/00 ,  A61Q19/08 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/0625 ,  C12N5/0629 ,  C12N5/0656 ,  C12N5/069 ,  C12N2502/1323 ,  C12P21/00 ,  A61K2300/00

摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.

摘要翻译： 描述了包含调节细胞培养基组合物和使用方法的新产品。 本发明的条件细胞培养基组合物可以由任何已知的定义或未定义的培养基组成，并且可以使用任何真核细胞类型进行调节。 一旦本发明的细胞培养基被调节，它可以在任何状态下使用。 条件培养基的物理实施方案包括但不限于液体或固体，冷冻，冻干或干燥成粉末。 另外，介质配制有药学上可接受的载体作为用于内部施用的载体，直接施用于食品或产品，或用药膏或软膏配制用于局部应用。 此外，可以进一步处理介质以浓缩或减少介质中包含的一种或多种因素或组分。

公开(公告)号：US20160102292A1

公开(公告)日：2016-04-14

申请号：US14715342

申请日：2015-05-18

申请人： CELLRESEARCH CORPORATION PTE LTD

发明人： Toan-Thang PHAN

IPC分类号： C12N5/077 ,  A61K35/32

CPC分类号： C12N5/0655 ,  A61K35/32 ,  A61L27/18 ,  A61L27/3633 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/56 ,  A61L27/60 ,  C12N5/0605 ,  C12N5/0629 ,  C12N5/0668 ,  C12N5/0676 ,  C12N5/0698 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2506/02 ,  C12N2506/1369 ,  C12N2533/54 ,  C08L69/00

摘要： The present invention relates to the generation of a chondrocyte using mesenchymal stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such chondrocytes.

摘要翻译： 本发明涉及使用从脐带羊膜获得的间充质干细胞/祖细胞和软骨细胞的治疗用途来产生软骨细胞。

公开(公告)号：US09295543B2

公开(公告)日：2016-03-29

申请号：US11375950

申请日：2006-03-15

申请人： B. Lynn Allen-Hoffmann ,  Cathy Ann-Rasmussen Ivarie ,  John M. Centanni

发明人： B. Lynn Allen-Hoffmann ,  Cathy Ann-Rasmussen Ivarie ,  John M. Centanni

IPC分类号： C12N5/00 ,  A61F2/10 ,  C12N5/071

CPC分类号： A61F2/105 ,  C12N5/0629 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323

摘要： The present invention relates to in vitro cultured skin substitutes. In particular, the present invention relates to compositions and methods for the development of cultured skin substitutes using NIKS cells.

公开(公告)号：US09085755B2

公开(公告)日：2015-07-21

申请号：US13781135

申请日：2013-02-28

申请人： CELLRESEARCH CORPORATION PTE LTD

发明人： Toan-Thang Phan ,  Ivor Jiun Lim

IPC分类号： C12N5/073 ,  C12N5/0735 ,  C12N5/071 ,  C12N5/0775

CPC分类号： A61K35/28 ,  A61K9/0014 ,  A61K9/06 ,  A61K35/36 ,  A61K35/51 ,  C12N5/0603 ,  C12N5/0605 ,  C12N5/0606 ,  C12N5/0629 ,  C12N5/063 ,  C12N5/0668 ,  C12N2506/025 ,  C12N2506/1392

摘要： The present invention relates to a method for isolating stem/progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem/progenitor cells from the tissue cultures. The isolated stem cell cells can have embryonic stem cell-like properties and can be used for various therapeutic purposes. In one embodiment, the invention relates to the isolation and cultivation of stem cells such as epithelial and/or mesenchymal stem/progenitor cells under conditions allowing the cells to undergo mitotic expansion. Furthermore, the invention is directed to a method for the differentiation of the isolated stem/progenitor cells into epithelial and/or mesenchymal cells.

摘要翻译： 本发明涉及一种从脐带羊膜中分离干细胞/祖细胞的方法，其中，该方法包括将羊膜与其他脐带组分体外分离，在允许细胞增殖的条件下培养羊膜组织 ，并从组织培养物中分离茎/祖细胞。 分离的干细胞细胞可具有胚胎干细胞样特性，可用于各种治疗目的。 在一个实施方案中，本发明涉及在允许细胞进行有丝分裂扩张的条件下分离和培养干细胞，例如上皮和/或间充质干细胞/祖细胞。 此外，本发明涉及将分离的干/祖细胞分化成上皮和/或间充质细胞的方法。

公开(公告)号：US20150050251A1

公开(公告)日：2015-02-19

申请号：US14521583

申请日：2014-10-23

申请人： Stemnion, Inc.

发明人： Catherine J Trumpower ,  Charlotte A. Emig

IPC分类号： A61K35/50

CPC分类号： A61K35/50 ,  A61K35/12 ,  C12N5/0605 ,  C12N5/0629 ,  C12N2501/11 ,  C12N2502/02

摘要： The invention is directed to methods for the treatment of wounds. Such methods utilize novel compositions, including but not limited to amnion-derived multipotent cells (herein referred to as AMP cells), conditioned media derived therefrom (herein referred to as amnion-derived cellular cytokine suspension or ACCS), cell lysates derived therefrom, cell products derived therefrom, each alone or in combination.

摘要翻译： 本发明涉及治疗伤口的方法。 这些方法利用新的组合物，包括但不限于羊膜来源的多潜能细胞（本文称为AMP细胞），由其衍生的条件培养基（本文称为羊膜来源的细胞细胞因子悬浮液或ACCS），由其衍生的细胞裂解物，细胞 从其衍生的产物，各自单独或组合。