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公开(公告)号：US20090043075A1
公开(公告)日：2009-02-12
申请号：US12172395
申请日：2008-07-14
申请人： Albert W. Alsop , Qiong Cheng , Linda Jane Decarolis , Stephen R. Fahnestock , Tanja Maria Gruber , Pierre E. Rouviere
发明人： Albert W. Alsop , Qiong Cheng , Linda Jane Decarolis , Stephen R. Fahnestock , Tanja Maria Gruber , Pierre E. Rouviere
CPC分类号： C12P21/02 , C07K2319/00 , C12P21/06
摘要： The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.
摘要翻译： 本发明涉及以包含溶解性标签的融合蛋白形式的目标肽的重组表达。 融合蛋白包含由可切割肽序列分开的至少两个部分，其中一部分缺少半胱氨酸残基，第二部分包含有效数目的可交联半胱氨酸残基。 细胞裂解和分离融合蛋白后，融合蛋白随后被切割成第一和第二部分的混合物。 氧化交联用于选择性沉淀两部分之一，以促进目的肽的简单和有效分离。
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公开(公告)号：US07951559B2
公开(公告)日：2011-05-31
申请号：US12172395
申请日：2008-07-14
申请人： Albert W. Alsop , Qiong Cheng , Linda Jane Solomon , Stephen R. Fahnestock , Tanja Maria Gruber , Pierre E. Rouviere
发明人： Albert W. Alsop , Qiong Cheng , Linda Jane Solomon , Stephen R. Fahnestock , Tanja Maria Gruber , Pierre E. Rouviere
CPC分类号： C12P21/02 , C07K2319/00 , C12P21/06
摘要： The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.
摘要翻译： 本发明涉及以包含溶解性标签的融合蛋白形式的目标肽的重组表达。 融合蛋白包含由可切割肽序列分开的至少两个部分，其中一部分缺少半胱氨酸残基，第二部分包含有效数目的可交联半胱氨酸残基。 在融合蛋白的细胞溶解和分离后，随后将融合蛋白切割成第一和第二部分的混合物。 氧化交联用于选择性沉淀两部分之一，以促进目的肽的简单和有效分离。
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公开(公告)号：US08617843B2
公开(公告)日：2013-12-31
申请号：US13080917
申请日：2011-04-06
申请人： Albert W. Alsop , Qiong Cheng , Linda Jane Solomon , Stephen R. Fahnestock , Tanja Maria Gruber , Pierre E. Rouviere
发明人： Albert W. Alsop , Qiong Cheng , Linda Jane Solomon , Stephen R. Fahnestock , Tanja Maria Gruber , Pierre E. Rouviere
CPC分类号： C12P21/02 , C07K2319/00 , C12P21/06
摘要： The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.
摘要翻译： 本发明涉及以包含溶解性标签的融合蛋白形式的目标肽的重组表达。 融合蛋白包含由可切割肽序列分开的至少两个部分，其中一部分缺少半胱氨酸残基，第二部分包含有效数目的可交联半胱氨酸残基。 细胞裂解和分离融合蛋白后，融合蛋白随后被切割成第一和第二部分的混合物。 氧化交联用于选择性沉淀两部分之一，以促进目的肽的简单和有效分离。
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公开(公告)号：US08403040B2
公开(公告)日：2013-03-26
申请号：US12833018
申请日：2010-07-09
摘要： A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.
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公开(公告)号：US08371376B2
公开(公告)日：2013-02-12
申请号：US12833050
申请日：2010-07-09
CPC分类号： C09K8/582
摘要： A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.
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公开(公告)号：US08183417B2
公开(公告)日：2012-05-22
申请号：US13035246
申请日：2011-02-25
申请人： Dennis N. Adkesson , Albert W. Alsop , Tyler T. Ames , Luis A. Chu , James M. Disney , Bryan C. Dravis , Patrick Fitzgibbon , James M. Gaddy , F. Glenn Gallagher , William F. Lehnhardt , Jefferson C. Lievense , Michael L. Luyben , Mayis Seapan , Robert E. Trotter , Gregory M. Wenndt , Eugene K. Yu
发明人： Dennis N. Adkesson , Albert W. Alsop , Tyler T. Ames , Luis A. Chu , James M. Disney , Bryan C. Dravis , Patrick Fitzgibbon , James M. Gaddy , F. Glenn Gallagher , William F. Lehnhardt , Jefferson C. Lievense , Michael L. Luyben , Mayis Seapan , Robert E. Trotter , Gregory M. Wenndt , Eugene K. Yu
CPC分类号： B01D61/027 , B01D3/002 , B01D3/143 , B01D3/145 , B01D61/04 , B01D61/142 , B01D61/145 , B01D61/147 , B01D61/58 , B01D2311/04 , C07C29/76 , C07C29/80 , C12P7/18 , B01D2311/2623 , C07C31/205
摘要： A process for purifying 1,3-propanediol from the fermentation broth of a cultured E. coli that has been bioengineered to synthesize 1,3-propanediol from sugar is provided. The basic process entails filtration, ion exchange and distillation of the fermentation broth product stream, preferably including chemical reduction of the product during the distillation procedure. Also provided are highly purified compositions of 1,3-propanediol.
摘要翻译： 本发明提供从已经生物工程化从糖中合成1,3-丙二醇的培养的大肠杆菌的发酵液中纯化1,3-丙二醇的方法。 基本过程需要发酵肉汤产品流的过滤，离子交换和蒸馏，优选在蒸馏过程中包括产物的化学还原。 还提供了高纯度的1,3-丙二醇组合物。
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公开(公告)号：US20120006542A1
公开(公告)日：2012-01-12
申请号：US12833070
申请日：2010-07-09
IPC分类号： E21B43/22
摘要： A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.
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公开(公告)号：US20120006541A1
公开(公告)日：2012-01-12
申请号：US12833065
申请日：2010-07-09
IPC分类号： E21B43/22
摘要： A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.
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公开(公告)号：US20110152583A1
公开(公告)日：2011-06-23
申请号：US13035246
申请日：2011-02-25
申请人： Dennis Michael Adkesson , Albert W. Alsop , Tyler T. Ames , Luis Alberto Chu , James M. Disney , Bryan C. Dravis , Patrick Fitzgibbon , James M. Gaddy , F. Glenn Gallagher , William F. Lehnhardt , Jefferson C. Lievense , Michael L. Luyben , Mayis Seapan , Robert E. Trotter , Gregory M. Wenndt , Eugene K. Yu
发明人： Dennis Michael Adkesson , Albert W. Alsop , Tyler T. Ames , Luis Alberto Chu , James M. Disney , Bryan C. Dravis , Patrick Fitzgibbon , James M. Gaddy , F. Glenn Gallagher , William F. Lehnhardt , Jefferson C. Lievense , Michael L. Luyben , Mayis Seapan , Robert E. Trotter , Gregory M. Wenndt , Eugene K. Yu
IPC分类号： C07C29/80
CPC分类号： B01D61/027 , B01D3/002 , B01D3/143 , B01D3/145 , B01D61/04 , B01D61/142 , B01D61/145 , B01D61/147 , B01D61/58 , B01D2311/04 , C07C29/76 , C07C29/80 , C12P7/18 , B01D2311/2623 , C07C31/205
摘要： A process for purifying 1,3-propanediol from the fermentation broth of a cultured E. coli that has been bioengineered to synthesize 1,3-propanediol from sugar is provided. The basic process entails filtration, ion exchange and distillation of the fermentation broth product stream, preferably including chemical reduction of the product during the distillation procedure. Also provided are highly purified compositions of 1,3-propanediol.
摘要翻译： 本发明提供从已经生物工程化从糖中合成1,3-丙二醇的培养的大肠杆菌的发酵液中纯化1,3-丙二醇的方法。 基本过程需要发酵肉汤产品流的过滤，离子交换和蒸馏，优选在蒸馏过程中包括产物的化学还原。 还提供了高纯度的1,3-丙二醇组合物。
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公开(公告)号：US20110152581A1
公开(公告)日：2011-06-23
申请号：US13035261
申请日：2011-02-25
申请人： Dennis Michael ADKESSON , Albert W. Alsop , Tyler T. Ames , Luis Alberto Chu , James M. Disney , Bryan C. Dravis , Patrick Fitzgibbon , James M. Gaddy , F. Glenn Gallagher , William F. Lehnhardt , Jefferson C. Lievense , Michael L. Luyben , Mayis Seapan , Robert E. Trotter , Gregory M. Wenndt , Eugene K. Yu
发明人： Dennis Michael ADKESSON , Albert W. Alsop , Tyler T. Ames , Luis Alberto Chu , James M. Disney , Bryan C. Dravis , Patrick Fitzgibbon , James M. Gaddy , F. Glenn Gallagher , William F. Lehnhardt , Jefferson C. Lievense , Michael L. Luyben , Mayis Seapan , Robert E. Trotter , Gregory M. Wenndt , Eugene K. Yu
IPC分类号： C07C31/20
CPC分类号： B01D61/027 , B01D3/002 , B01D3/143 , B01D3/145 , B01D61/04 , B01D61/142 , B01D61/145 , B01D61/147 , B01D61/58 , B01D2311/04 , C07C29/76 , C07C29/80 , C12P7/18 , B01D2311/2623 , C07C31/205
摘要： A process for purifying 1,3-propanediol from the fermentation broth of a cultured E. coil that has been bioengineered to synthesize 1,3-propanediol from sugar is provided. The basic process entails filtration, ion exchange and distillation of the fermentation broth product stream, preferably including chemical reduction of the product during the distillation procedure. Also provided are highly purified compositions of 1,3-propanediol.
摘要翻译： 提供了从生物工程合成1,3-丙二醇的培养的E.线圈的发酵液中纯化1,3-丙二醇的方法。 基本过程需要发酵肉汤产品流的过滤，离子交换和蒸馏，优选在蒸馏过程中包括产物的化学还原。 还提供了高纯度的1,3-丙二醇组合物。
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