Protein kinase and phosphatase substrates and multiplex assays for identifying their activities
    1.
    发明申请
    Protein kinase and phosphatase substrates and multiplex assays for identifying their activities 审中-公开
    蛋白激酶和磷酸酶底物以及用于鉴定其活性的多重测定

    公开(公告)号:US20060046277A1

    公开(公告)日:2006-03-02

    申请号:US10931251

    申请日:2004-09-01

    IPC分类号: C12Q1/48 C12N9/12

    CPC分类号: C12Q1/42 C12Q1/485

    摘要: The present invention provides protein kinase and protein phosphatase substrates, methods of detecting protein kinases and protein phosphatases, and kits for detection of protein kinases and protein phosphatases. The substrates, methods, and kits use multiple substrates that can easily be differentiated from all other substrates, thus enabling rapid and easy detection of protein kinase or protein phosphatase activities. The invention also provides methods of directionally cloning nucleic acids.

    摘要翻译: 本发明提供蛋白激酶和蛋白磷酸酶底物,检测蛋白激酶和蛋白磷酸酶的方法,以及用于检测蛋白激酶和蛋白磷酸酶的试剂盒。 底物,方法和试剂盒使用可以容易地从所有其他底物分化的多种底物,因此能够快速和容易地检测蛋白激酶或蛋白磷酸酶活性。 本发明还提供了定向克隆核酸的方法。