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1.发明授权Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores 失效使用带有花青染料作为供体发色团的能量转移标记进行测序和检测的方法
公开(公告)号：US6150107A
公开(公告)日：2000-11-21
申请号：US164800
申请日：1998-10-01
CPC分类号： C12Q1/6818 , C12Q1/6869
摘要： Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
摘要翻译： 能量转移标记中使用花青染料作为供体荧光团，其中光能被供体荧光团吸收并转移到受体荧光团，受体荧光基团通过发射荧光检测反应。 花青染料赋予标签异常高的灵敏度，从而改善其在各种生物化学方法，特别是核酸测序，核酸片段大小和相关方法中的用途。
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2.发明授权Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels 失效在能量转移标签中具有高吸光度横截面作为供体发色团的花青染料
公开(公告)号：US5853992A
公开(公告)日：1998-12-29
申请号：US726178
申请日：1996-10-04
CPC分类号： C12Q1/6818 , C12Q1/6869
摘要： Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
摘要翻译： 能量转移标记中使用花青染料作为供体荧光团，其中光能被供体荧光团吸收并转移到受体荧光团，受体荧光基团通过发射荧光检测反应。 花青染料赋予标签异常高的灵敏度，从而改善其在各种生物化学方法，特别是核酸测序，核酸片段大小和相关方法中的用途。
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3.发明授权Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation 失效通过荧光共振能量转移标记产生检测核苷酸序列变异公开(公告)号：US06573047B1
公开(公告)日：2003-06-03
申请号：US09547292
申请日：2000-04-11
IPC分类号： C12Q168
CPC分类号： C12Q1/6858 , C12Q2565/101 , C12Q2535/125
摘要： The present invention provides methods and kits for identifying nucleotides at a variant site in a target molecule by forming fluorescence resonance energy transfer labeled product. The location and type of fluorescent labels in the labeled product provide strong signals and relatively high spectral purity that facilitate detection. Utilizing various secondary labels and different combinations of acceptor and donor labels, certain methods permit multiple analyses to be conducted simultaneously and at high throughput. The methods can be used in a variety of applications such as analyzing point mutations and single nucleotide polymorphisms (SNPs). In addition, the methods have utility in other applications in which specific sequence information is of value, including detection of pathogens, paternity disputes, prenatal testing and forensic analysis.
摘要翻译： 本发明提供用于通过形成荧光共振能量转移标记产物来鉴定靶分子中变体位点处的核苷酸的方法和试剂盒。 标记产品中荧光标记的位置和类型提供强信号和较高的光谱纯度，便于检测。 利用各种二级标签和受体和供体标签的不同组合，某些方法允许同时进行多次分析并以高产量进行。 该方法可用于各种应用，如分析点突变和单核苷酸多态性（SNPs）。 此外，这些方法在其他应用中具有实用价值，其中具体的序列信息是有价值的，包括病原体的检测，亲子鉴定，产前检测和法医分析。
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