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1.发明授权公开(公告)号：US06428667B1
公开(公告)日：2002-08-06
申请号：US09686147
申请日：2000-10-10
IPC分类号： B01D5702
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747 , Y10T436/143333 , C12Q2565/102 , C12Q2563/173
摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。
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2.发明授权Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity 失效具有生物识别特异性的重组链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白公开(公告)号：US06391590B1
公开(公告)日：2002-05-21
申请号：US07780717
申请日：1991-10-21
IPC分类号： C12N1562
CPC分类号： C07H21/04 , C07K14/36 , C07K14/825 , C07K2319/00 , C07K2319/22
摘要： Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.
摘要翻译： 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。
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3.发明授权Electrochemical detector integrated on microfabricated capilliary electrophoresis chips 失效电化学检测器集成在微细毛细管电泳芯片上
公开(公告)号：US6045676A
公开(公告)日：2000-04-04
申请号：US916557
申请日：1997-08-22
IPC分类号： G01N27/447 , G01N30/60 , G01N37/00 , G01N27/26
CPC分类号： G01N27/4473 , G01N27/44791 , G01N30/6095
摘要： A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.
摘要翻译： 一种微细毛细管电泳芯片，其包括用于检测在毛细管中分离的分子的整体薄膜电化学检测器。
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公开(公告)号：US4859582A
公开(公告)日：1989-08-22
申请号：US701324
申请日：1985-02-13
申请人： Lubert Stryer , Alexander N. Glazer , Vernon T. Oi
发明人： Lubert Stryer , Alexander N. Glazer , Vernon T. Oi
IPC分类号： G01N33/533
CPC分类号： G01N33/533 , Y10S436/80 , Y10S436/805 , Y10S530/807
摘要： Sensitive detection techniques and compositions for such techniques are provided by employing fluorescent proteins having bilin prosthetic groups as labels. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjunction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.
摘要翻译： 通过使用具有胆汁假体基团的荧光蛋白作为标记来提供这种技术的敏感检测技术和组合物。 含有蛋白质的蛋白质可以与配体或受体结合，用于涉及配体 - 受体结合的系统，用于配体和受体的分析，检测或分离。 特别地，一种或多种含胆红蛋白的蛋白质可以用作彼此结合的标记或用于定义天然存在的聚集子集的其它荧光剂。 细胞。
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5.发明授权Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display 有权多功能重组藻胆蛋白基荧光结构和phycobilisome显示公开(公告)号：US07176000B2
公开(公告)日：2007-02-13
申请号：US10617012
申请日：2003-07-10
申请人： Alexander N. Glazer , Yuping Cai
发明人： Alexander N. Glazer , Yuping Cai
CPC分类号： C07K14/415 , C07K2319/00 , Y10S435/822
摘要： The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein. including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
摘要翻译： 本发明提供了多功能融合构建体，其迅速并入大分子结构例如藻糖酵母中，使得融合蛋白彼此分离并且不能自相关。 本发明提供了在寡聚藻胆蛋白上显示功能性多肽结构域的方法和组合物。 包括包含功能性显示结构域的融合蛋白和掺入功能性寡聚藻胆蛋白的功能性藻胆蛋白结构域。 融合蛋白提供新的特异性标记试剂。
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6.发明授权Engineering of living cells for the expression of holo-phycobiliprotein-based constructs 有权用于表达全脂蛋白脂蛋白的构建体的活细胞工程公开(公告)号：US06740507B2
公开(公告)日：2004-05-25
申请号：US09919486
申请日：2001-07-31
申请人： Alexander N. Glazer , Aaron J. Tooley , Yuping Cai
发明人： Alexander N. Glazer , Aaron J. Tooley , Yuping Cai
IPC分类号： C12P2104
CPC分类号： C12N9/0095 , C07K14/43595 , C07K2319/21 , C07K2319/60 , C12N9/88 , C12N15/52 , C12N15/62
摘要： Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.
摘要翻译： 描述表达荧光全血蓝蛋白融合蛋白的重组细胞及其使用方法。 所述细胞包含bilin，重组bilin还原酶，包含相应的载脂蛋白结构域的融合蛋白的载脂蛋白 - 磷脂蛋白融合蛋白前体，以及组分反应形成全血蓝蛋白融合蛋白的重组体藻胆蛋白结构域 - 胆固醇裂解酶 。 还描述了基于全藻蛋白脂蛋白的转录报道细胞和测定法，其中细胞条件地表达异源细胞，荧光，第一全息型蛋白脂蛋白结构域。
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7.发明授权Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores 失效使用带有花青染料作为供体发色团的能量转移标记进行测序和检测的方法
公开(公告)号：US6150107A
公开(公告)日：2000-11-21
申请号：US164800
申请日：1998-10-01
CPC分类号： C12Q1/6818 , C12Q1/6869
摘要： Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
摘要翻译： 能量转移标记中使用花青染料作为供体荧光团，其中光能被供体荧光团吸收并转移到受体荧光团，受体荧光基团通过发射荧光检测反应。 花青染料赋予标签异常高的灵敏度，从而改善其在各种生物化学方法，特别是核酸测序，核酸片段大小和相关方法中的用途。
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8.发明授权DNA complexes with dyes designed for energy transfer as fluorescent markers 失效DNA复合物与设计用于能量转移的染料作为荧光标记物
公开(公告)号：US5401847A
公开(公告)日：1995-03-28
申请号：US9704
申请日：1993-01-27
IPC分类号： G01N33/58 , C07D221/12 , C07D405/12 , C07D417/06 , C07D417/14 , C07H21/04 , C09B23/04 , C12N15/09 , C12Q1/68 , G01N27/447 , C07H19/00 , G01N27/26
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , C12Q1/6818 , G01N27/44726 , G01N27/44747
摘要： Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.
摘要翻译： 提供了异源多聚体荧光团用于结合DNA，其允许在电分离中检测DNA并制备具有高荧光效率和大的斯托克斯位移的探针。 此外，通过适当选择荧光分子，可以使用单个窄波长带激发光源，同时获得具有足够分离的荧光发射以易于鉴别。
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9.发明授权Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display 有权多功能重组藻胆蛋白基荧光结构和phycobilisome显示公开(公告)号：US07169582B2
公开(公告)日：2007-01-30
申请号：US10617208
申请日：2003-07-10
申请人： Alexander N. Glazer , Yuping Cai
发明人： Alexander N. Glazer , Yuping Cai
CPC分类号： C07K14/415 , C07K2319/00 , Y10S435/822
摘要： The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
摘要翻译： 本发明提供了多功能融合构建体，其迅速并入大分子结构例如藻糖酵母中，使得融合蛋白彼此分离并且不能自相关。 本发明提供了用于在寡聚藻胆蛋白上显示功能性多肽结构域的方法和组合物，包括融合蛋白，其包含功能性显示结构域和掺入功能性寡聚藻胆蛋白的功能性藻胆蛋白结构域。 融合蛋白提供新的特异性标记试剂。
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10.发明授权公开(公告)号：US06280933B1
公开(公告)日：2001-08-28
申请号：US08966398
申请日：1997-11-07
IPC分类号： C12Q168
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747 , Y10T436/143333 , C12Q2565/102 , C12Q2563/173
摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。
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