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1.发明申请DUAL CHARGING SYSTEM FOR SELECTIVELY INTRODUCING NON-NATIVE AMINO ACIDS INTO PROTEINS USING AN IN VITRO SYNTHESIS METHOD 审中-公开使用体外合成方法选择性地将非氨基酸引入蛋白质的双重充电系统公开(公告)号：US20100184134A1
公开(公告)日：2010-07-22
申请号：US12685544
申请日：2010-01-11
申请人： Alexei M. Voloshin , James F. Zawada , Daniel Gold , Christopher James Murray , James Edward Rozzelle , Nathan Uter , Gang Yin
发明人： Alexei M. Voloshin , James F. Zawada , Daniel Gold , Christopher James Murray , James Edward Rozzelle , Nathan Uter , Gang Yin
IPC分类号： C12P21/06
摘要： This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.
摘要翻译： 本发明提供了使用无细胞合成系统将非天然氨基酸并入蛋白质的预选位置的新方法。 这些方法涉及使用由遗传密码编码的非正交的天然异位感知有义tRNA。 这样的方法允许通过使用有义密码子掺入许多非天然氨基酸，而不必依赖正交的tRNA-合成酶对。
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2.发明授权Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system 有权单体充电系统，用于使用体外蛋白质合成系统选择性地将非天然氨基酸引入蛋白质公开(公告)号：US08778631B2
公开(公告)日：2014-07-15
申请号：US12685795
申请日：2010-01-12
申请人： Alexei M. Voloshin , James F. Zawada , Daniel Gold , Christopher James Murray , James Edward Rozzelle , Nathan Uter , Gang Yin
发明人： Alexei M. Voloshin , James F. Zawada , Daniel Gold , Christopher James Murray , James Edward Rozzelle , Nathan Uter , Gang Yin
摘要： This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.
摘要翻译： 本发明提供了使用无细胞合成系统将非天然氨基酸并入蛋白质的预选位置的新方法。 这些方法涉及使用由遗传密码编码的非正交的天然异位感知有义tRNA。 这样的方法允许通过使用有义密码子掺入许多非天然氨基酸，而不必依赖正交的tRNA-合成酶对。
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3.发明申请MONO CHARGING SYSTEM FOR SELECTIVELY INTRODUCING NON-NATIVE AMINO ACIDS INTO PROTEINS USING AN IN VITRO PROTEIN SYNTHESIS SYSTEM 有权使用维生素蛋白合成系统选择性地将非氨基酸引入蛋白质的单收取系统公开(公告)号：US20100184135A1
公开(公告)日：2010-07-22
申请号：US12685795
申请日：2010-01-12
申请人： Alexei M. Voloshin , James F. Zawada , Daniel Gold , Christopher James Murray , James Edward Rozzelle , Nathan Uter , Gang Yin
发明人： Alexei M. Voloshin , James F. Zawada , Daniel Gold , Christopher James Murray , James Edward Rozzelle , Nathan Uter , Gang Yin
摘要： This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.
摘要翻译： 本发明提供了使用无细胞合成系统将非天然氨基酸并入蛋白质的预选位置的新方法。 这些方法涉及使用由遗传密码编码的非正交的天然异位感知有义tRNA。 这样的方法允许通过使用有义密码子掺入许多非天然氨基酸，而不必依赖正交的tRNA-合成酶对。
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公开(公告)号：US20090145500A1
公开(公告)日：2009-06-11
申请号：US12317220
申请日：2008-12-18
IPC分类号： E03C1/02
CPC分类号： E03B1/042 , E03B1/04 , E03B1/048 , E03B2001/045 , E03C1/02 , F24D17/0078 , Y10T29/49716 , Y10T137/6497 , Y10T137/86083
摘要： A valve assembly (18) including: a housing (41) having a water supply inlet (48), a hot water outlet (49), a cold water outlet (50), a hot water flow passage (64) between said water supply inlet and said hot water outlet and a cold water flow passage (65) between said water supply inlet and said cold water outlet; hot water valve means (51) in said housing adapted to open said hot water flow passage (64) in response to entry of water above a predetermined temperature into said housing through said water supply inlet and to close said hot water flow passage in response to entry of water below said predetermined temperature into said housing through said water supply inlet; first cold water valve means (51) adapted to open said cold water flow passage (65) at a first position in response to entry of water below said predetermined temperature into said housing through said water supply inlet and to close said cold water flow passage at said first position in response to entry of water above said predetermined temperature into said housing through said water supply inlet; second cold water valve means (80) in series with said first cold water valve means and adapted to open said cold water flow passage (65) at a second position in response to a predetermined drop in pressure at said hot water outlet and to close said cold water flow passage at said second position in response to a predetermined increase in pressure at said hot water outlet.
摘要翻译： 一种阀组件（18），包括：具有供水入口（48）的壳体（41），热水出口（49），冷水出口（50），所述供水口 入口和所述热水出口以及所述供水入口和所述冷水出口之间的冷水流动通道（65） 所述壳体中的热水阀装置（51）适于响应于通过所述供水入口将水超过预定温度进入所述壳体而打开所述热水流动通道（64），并响应于 通过所述供水入口将水低于所述预定温度进入所述壳体; 第一冷水阀装置（51），其适于在第一位置打开所述冷水流动通道（65），以响应于通过所述供水入口将水低于所述预定温度进入所述壳体并关闭所述冷水流动通道 响应于高于所述预定温度的水通过所述供水入口进入所述壳体的所述第一位置; 第二冷水阀装置（80），其与所述第一冷水阀装置串联并且适于响应于所述热水出口处的预定压力下降而在第二位置打开所述冷水流动通道（65），并且关闭所述 响应于所述热水出口处的压力的预定增加，在所述第二位置处的冷水流动通道。
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公开(公告)号：US07934663B2
公开(公告)日：2011-05-03
申请号：US12317220
申请日：2008-12-18
IPC分类号： G05D23/13
CPC分类号： E03B1/042 , E03B1/04 , E03B1/048 , E03B2001/045 , E03C1/02 , F24D17/0078 , Y10T29/49716 , Y10T137/6497 , Y10T137/86083
摘要： A valve assembly (18) including: a housing (41) having a water supply inlet (48), a hot water outlet (49), a cold water outlet (50), a hot water flow passage (64) between said water supply inlet and said hot water outlet and a cold water flow passage (65) between said water supply inlet and said cold water outlet; hot water valve means (51) in said housing adapted to open said hot water flow passage (64) in response to entry of water above a predetermined temperature into said housing through said water supply inlet and to close said hot water flow passage in response to entry of water below said predetermined temperature into said housing through said water supply inlet; first cold water valve means (51) adapted to open said cold water flow passage (65) at a first position in response to entry of water below said predetermined temperature into said housing through said water supply inlet and to close said cold water flow passage at said first position in response to entry of water above said predetermined temperature into said housing through said water supply inlet; second cold water valve means (80) in series with said first cold water valve means and adapted to open said cold water flow passage (65) at a second position in response to a predetermined drop in pressure at said hot water outlet and to close said cold water flow passage at said second position in response to a predetermined increase in pressure at said hot water outlet.
摘要翻译： 一种阀组件（18），包括：具有供水入口（48）的壳体（41），热水出口（49），冷水出口（50），所述供水口 入口和所述热水出口以及所述供水入口和所述冷水出口之间的冷水流动通道（65） 所述壳体中的热水阀装置（51）适于响应于通过所述供水入口将水超过预定温度进入所述壳体而打开所述热水流动通道（64），并响应于 通过所述供水入口将水低于所述预定温度进入所述壳体; 第一冷水阀装置（51），其适于在第一位置打开所述冷水流动通道（65），以响应于通过所述供水入口将水低于所述预定温度进入所述壳体并关闭所述冷水流动通道 响应于高于所述预定温度的水通过所述供水入口进入所述壳体的所述第一位置; 第二冷水阀装置（80），其与所述第一冷水阀装置串联并且适于响应于所述热水出口处的预定压力下降而在第二位置打开所述冷水流动通道（65），并且关闭所述 响应于所述热水出口处的压力的预定增加，在所述第二位置处的冷水流动通道。
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公开(公告)号：US07487923B2
公开(公告)日：2009-02-10
申请号：US10551362
申请日：2004-03-31
CPC分类号： E03B1/042 , E03B1/04 , E03B1/048 , E03B2001/045 , E03C1/02 , F24D17/0078 , Y10T29/49716 , Y10T137/6497 , Y10T137/86083
摘要： A valve assembly (18) including: a housing (41) having a water supply inlet (48), a hot water outlet (49), a cold water outlet (50), a hot water flow passage (64) between said water supply inlet and said hot water outlet and a cold water flow passage (65) between said water supply inlet and said cold water outlet; hot water valve means (51) in said housing adapted to open said hot water flow passage (64) in response to entry of water above a predetermined temperature into said housing through said water supply inlet and to close said hot water flow passage in response to entry of water below said predetermined temperature into said housing through said water supply inlet; first cold water valve means (51) adapted to open said cold water flow passage (65) at a first position in response to entry of water below said predetermined temperature into said housing through said water supply inlet and to close said cold water flow passage at said first position in response to entry of water above said predetermined temperature into said housing through said water supply inlet; second cold water valve means (80) in series with said first cold water valve means and adapted to open said cold water flow passage (65) at a second position in response to a predetermined drop in pressure at said hot water outlet and to close said cold water flow passage at said second position in response to a predetermined increase in pressure at said hot water outlet.
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