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10 条记录 (耗时 0.037 秒)

    USE OF DNA GYRASE INHIBITORS FOR IN VITRO POLYPEPTIDE SYNTHESIS REACTIONS
    4.
    发明申请
    USE OF DNA GYRASE INHIBITORS FOR IN VITRO POLYPEPTIDE SYNTHESIS REACTIONS 审中-公开
    DNA多糖抑制剂在体外多肽合成反应中的应用

    公开(公告)号：US20100203587A1

    公开(公告)日：2010-08-12

    申请号：US12685989

    申请日：2010-01-12

    申请人： Alexei M. Voloshin ,  James F. Zawada ,  Daniel Gold

    发明人： Alexei M. Voloshin ,  James F. Zawada ,  Daniel Gold

    IPC分类号： C12P21/00 ,  C12N15/74

    CPC分类号： C12P21/02

    摘要： The present invention provides methods and compositions useful for in vitro polypeptide synthesis reactions. The methods involve the use of DNA gyrase inhibitors to prevent bacterial contamination in lysates used for in vitro production of polypeptides. The compositions include contamination-free cell lysates for in vitro protein synthesis reactions.

    摘要翻译： 本发明提供了可用于体外多肽合成反应的方法和组合物。 该方法涉及使用DNA促旋酶抑制剂来防止用于体外产生多肽的裂解物中的细菌污染。 组合物包括用于体外蛋白质合成反应的无污染细胞裂解物。

    Process for disassembling a brazed structure
    6.
    发明授权
    Process for disassembling a brazed structure 有权
    拆装钎焊结构的工艺

    公开(公告)号：US06655576B2

    公开(公告)日：2003-12-02

    申请号：US10107231

    申请日：2002-03-26

    申请人： Daniel Gold ,  Gary Dudek ,  Loretta Lietzan ,  James Moor ,  Will Glesener ,  Mike Harris

    发明人： Daniel Gold ,  Gary Dudek ,  Loretta Lietzan ,  James Moor ,  Will Glesener ,  Mike Harris

    IPC分类号： B23K1018

    CPC分类号： B23K1/0014 ,  B23K1/018 ,  B23K2101/02

    摘要： Brazed structures comprising hollow components having a closure component bonded thereto by a metallic brazing alloy for closing off an end of the hollow component are disassembled by locating a wire transport material in the hollow component and covering the open face of the hollow component with a wire fiber material. Heat is then applied to liquefy the brazing alloy which is drawn up into the metal wire fiber material by capillary affect created by the wire transport material.

    摘要翻译： 通过将丝网输送材料定位在中空部件中并用丝线纤维覆盖中空部件的开口面，分解包括具有通过用于封闭中空部件的端部的金属钎焊合金结合到其上的封闭部件的中空部件 材料。 然后施加热量以通过由电线传输材料产生的毛细管作用来液化被钎焊到金属线纤维材料中的钎焊合金。

    Register mark detection apparatus utilizing a first and second linear array of sensors arranged non-parallel allowing longitudinal and transverse monitoring
    7.
    发明授权
    Register mark detection apparatus utilizing a first and second linear array of sensors arranged non-parallel allowing longitudinal and transverse monitoring 失效
    使用传感器的第一和第二线性阵列的寄存器标志检测装置安排非平行允许的纵向和横向监视

    公开(公告)号：US5115141A

    公开(公告)日：1992-05-19

    申请号：US527809

    申请日：1990-05-24

    申请人： Daniel Gold

    发明人： Daniel Gold

    IPC分类号： B41F33/14 ,  B41F33/00 ,  G01B11/00

    CPC分类号： B41F33/0081

    摘要： Apparatus for detecting register marks includes one or more linear arrays of sensors (19, 20) arranged transverse to the direction of relative movement of a web (3) and the apparatus. Where there is more than one linear array (19, 20), they are arranged so that they are substantially non-parallel to allow both longitudinal and transverse monitoring of register marks on the web (3). A signal is generated on detection of a mark and processing means determine the sensor (19) or group of sensors which detected a mark and whether the marks are in register with those of other webs (3).

    Object-driven visualization mechanism for zoning information on a storage area network
    8.
    发明授权
    Object-driven visualization mechanism for zoning information on a storage area network 有权
    用于在存储区域网络上分区信息的对象驱动的可视化机制

    公开(公告)号：US07533169B1

    公开(公告)日：2009-05-12

    申请号：US10743370

    申请日：2003-12-22

    申请人： Daniel Gold ,  Kavita Gupta

    发明人： Daniel Gold ,  Kavita Gupta

    IPC分类号： G06F15/177

    CPC分类号： H04L67/1097 ,  G06F3/0601 ,  G06F11/0727 ,  G06F11/0751 ,  G06F2003/0697 ,  G06F2206/1008 ,  H04L41/12 ,  H04L63/101

    摘要： System and method for object-centric Storage Area Network (SAN) zoning visualization. Embodiments may provide graphical and/or textual displays and reporting of logical and/or physical zone and zone alias information for SAN objects to users or administrators of a SAN. SAN objects may include any logically or physically zonable SAN component. Embodiments may graphically and/or textually present logical and/or physical zoning information for any SAN object via real-time displays and/or reporting in response to selecting the object. All of the zones, and zone aliases, if any, of which a SAN object is a logical and/or physical member may be determined and displayed, along with other relevant information including the mechanisms by which the SAN object is logically zoned. Embodiments may provide mechanisms through which a user may navigate through the displayed zoning information to view zoning information for other SAN objects and/or zone-centric information for the zones and zone aliases.

    摘要翻译： 用于面向对象的存储区域网络（SAN）分区可视化的系统和方法。 实施例可以向SAN的用户或管理员提供用于SAN对象的逻辑和/或物理区域和区域别名信息的图形和/或文本显示和报告。 SAN对象可以包括任何逻辑或物理上可分区的SAN组件。 实施例可以响应于选择对象而通过实时显示和/或报告来图形地和/或文本地呈现任何SAN对象的逻辑和/或物理分区信息。 可以确定和显示其中SAN对象是逻辑和/或物理成员的所有区域和区域别名（如果有的话）以及包括SAN对象在逻辑上划分的机制的其他相关信息。 实施例可以提供用户可以通过显示的分区信息浏览以查看其他SAN对象的分区信息和/或区域和区域别名的区域中心信息的机制。

    Method and composition for altering a B cell mediated pathology
    9.
    发明申请
    Method and composition for altering a B cell mediated pathology 有权

    公开(公告)号：US20050202004A1

    公开(公告)日：2005-09-15

    申请号：US10842953

    申请日：2004-05-10

    申请人： Daniel Gold ,  Robert Shopes

    发明人： Daniel Gold ,  Robert Shopes

    IPC分类号： A61K38/17 ,  C12N5/08 ,  A61K39/395

    CPC分类号： C07K16/18 ,  A61K38/1774 ,  A61K38/193 ,  A61K38/195 ,  A61K39/39541 ,  A61K47/643 ,  A61K47/646 ,  A61K2039/505 ,  C07K2319/00 ,  C07K2319/30 ,  Y10S514/885 ,  A61K2300/00

    摘要： The present invention provides a method for altering a B cell mediated pathology in a patient. This method comprises administering a composition comprising at least one and/or two chimeric proteins. Each chimeric protein comprises at least a portion of either the VH or VL region of a immunoglobulin molecule from particular B cells from a patient having a B cell mediated pathology, and an immunoglobulin constant region. The genes encoding VH and/or VL regions and the genes encoding immunoglobulin constant regions are isolated and inserted into an expression vector. The chimeric proteins are produced by introducing the expression vectors into insect cell lines. The chimeric proteins are purified using antibody affinity columns, and then chemically conjugated to an immunogenic carrier, keyhole-limpet hemocyanin (KLH). Since the conjugates comprise chimeric proteins made specifically from particular B cells from a patient having B cell mediated pathology, when it is administered to such a patient, with or without a cytokine, such as granulocyte-macrophage-CSF, or a chemokine, it can induce immune responses to alter such a B cell mediated pathology.

    Method and composition for altering a B cell mediated pathology
    10.
    发明申请
    Method and composition for altering a B cell mediated pathology 有权
    用于改变B细胞介导的病理学的方法和组合物

    公开(公告)号：US20050238645A1

    公开(公告)日：2005-10-27

    申请号：US10843703

    申请日：2004-05-10

    申请人： Daniel Gold ,  Robert Shopes

    发明人： Daniel Gold ,  Robert Shopes

    IPC分类号： A61K38/17 ,  C12N5/08 ,  A61K39/395

    CPC分类号： C07K16/18 ,  A61K38/1774 ,  A61K38/193 ,  A61K38/195 ,  A61K39/39541 ,  A61K47/643 ,  A61K47/646 ,  A61K2039/505 ,  C07K2319/00 ,  C07K2319/30 ,  Y10S514/885 ,  A61K2300/00

    摘要： The present invention provides a method for altering a B cell mediated pathology in a patient. This method comprises administering a composition comprising at least one and/or two chimeric proteins. Each chimeric protein comprises at least a portion of either the VH or VL region of a immunoglobulin molecule from particular B cells from a patient having a B cell mediated pathology, and an immunoglobulin constant region. The genes encoding VH and/or VL regions and the genes encoding immunoglobulin constant regions are isolated and inserted into an expression vector. The chimeric proteins are produced by introducing the expression vectors into insect cell lines. The chimeric proteins are purified using antibody affinity columns, and then chemically conjugated to an immunogenic carrier, keyhole-limpet hemocyanin (KLH). Since the conjugates comprise chimeric proteins made specifically from particular B cells from a patient having B cell mediated pathology, when it is administered to such a patient, with or without a cytokine, such as granulocyte-macrophage-CSF, or a chemokine, it can induce immune responses to alter such a B cell mediated pathology.

    摘要翻译： 本发明提供了改变患者B细胞介导的病理学的方法。 该方法包括施用包含至少一种和/或两种嵌合蛋白质的组合物。 每个嵌合蛋白包含来自具有B细胞介导的病理学的患者的特定B细胞的免疫球蛋白分子的至少一部分V H H或V L L区，以及 免疫球蛋白恒定区。 编码V H区和/或V L区的基因和编码免疫球蛋白恒定区的基因被分离并插入到表达载体中。 通过将表达载体引入昆虫细胞系中产生嵌合蛋白。 使用抗体亲和柱纯化嵌合蛋白，然后化学偶联至免疫原性载体键孔血蓝蛋白（KLH）。 由于缀合物包含特异性地从具有B细胞介导的病理学的患者的特定B细胞制备的嵌合蛋白，当向具有或不具有细胞因子如粒细胞 - 巨噬细胞-SCF或趋化因子的这种患者施用时，可以 诱导免疫应答来改变这种B细胞介导的病理学。