公开(公告)号：US20200318166A1

公开(公告)日：2020-10-08

申请号：US16478448

申请日：2018-01-18

申请人： Altius Institute for Biomedical Sciences

发明人： John A. Stamatoyannopoulos ,  Shreeram Akilesh ,  Pavel Zrazhevskiy

IPC分类号： C12Q1/6816

摘要： Methods and apparatus to test and screen compounds in a multiplexed manner, using a mixture of genetically or functionally heterogeneous cells in common conditions.

公开(公告)号：US20220010366A1

公开(公告)日：2022-01-13

申请号：US17293869

申请日：2019-11-18

申请人： Altius Institute for Biomedical Sciences

发明人： John Stamatoyannopoulos ,  Pavel Zrazhevskiy

IPC分类号： C12Q1/6816

摘要： Provided herein are methods and compositions for use in detecting on-target and/or off-target cleavage of genomic DNA in a cell by a nuclease. The on-target and off-target cleavage is detected by using oligonucleotides that bind to the single-stranded 3′-overhang created at the cleavage site. The nuclease may be an engineered nuclease comprising a nucleic acid binding domain that binds to a target nucleic acid of interest.

公开(公告)号：US20210147922A1

公开(公告)日：2021-05-20

申请号：US17047456

申请日：2019-04-18

申请人： ALTIUS INSTITUTE FOR BIOMEDICAL SCIENCES

发明人： Fyodor Urnov ,  John A. Stamatoyannopoulos ,  Vivek Nandakumar ,  Pavel Zrazhevskiy ,  Shreeram Akilesh

IPC分类号： C12Q1/6841 ,  C12N15/10

摘要： The present disclosure provides methods and compositions for image based analysis and quantification of a protein load from protein (e.g., p53BP1) accumulation, induced by a cellular perturbation, such as administration of a genome editing tool comprising a DNA binding domain and a nuclease domain, a gene repressor, or a gene activator.