公开(公告)号：US11078464B2

公开(公告)日：2021-08-03

申请号：US14915630

申请日：2014-08-28

Applicant: AMGEN INC.

Inventor： Huiren Zhao ,  Grace Ki Jeong Lee ,  Thomas Wolfe ,  Cherylene Plewa ,  Jackie Z. Sheng

IPC: C12N7/00 ,  C12N15/86

Abstract: The present invention is directed to an in vitro method of producing a recombinant AAV virion in a mammalian host cell that comprises a functional adenoviral E1A gene and rAAV virions made by the method. Host cells are transfected with varying ratios of plasmids comprising the E1A gene and virions.

公开(公告)号：US11834683B2

公开(公告)日：2023-12-05

申请号：US17387896

申请日：2021-07-28

Applicant: Amgen Inc.

Inventor： Huiren Zhao ,  Grace Ki Jeong Lee ,  Thomas Wolfe ,  Cherylene Plewa ,  Jackie Z. Sheng

IPC: C12N7/00 ,  C12N15/86

CPC classification number: C12N7/00 ,  C12N15/86 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14152

Abstract: The present invention is directed to an in vitro method of producing a recombinant AAV virion in a mammalian host cell that comprises a functional adenoviral E1A gene (e.g., a HEK 293T cell) and rAAV virions made by the method.