公开(公告)号：US20220282228A1

公开(公告)日：2022-09-08

申请号：US17608437

申请日：2020-05-01

Applicant: Amyris, Inc.

Inventor： Andrew P. KLEIN ,  Sean LUND ,  Gale WICHMANN ,  Irina KORYAKINA ,  Kyle HOGAN ,  Svetlana BORISOVA

IPC: C12N9/02 ,  C12P19/56

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of Ro.KAH. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides including rebaudioside A, rebaudioside D, and rebaudioside M.