公开(公告)号：US20230304049A1

公开(公告)日：2023-09-28

申请号：US18022063

申请日：2021-08-19

Applicant: Amyris, Inc.

Inventor： Victor F. HOLMES ,  John E. HUNG ,  Irina A. KORYAKINA ,  Andrew P. KLEIN ,  Yi XIONG ,  William E. DRAPER ,  Hailley S. WARBINGTON

IPC: C12P7/42 ,  C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12N9/88 ,  C12N1/18

CPC classification number: C12P7/42 ,  C12N9/0004 ,  C12N9/1029 ,  C12N9/93 ,  C12N9/88 ,  C12Y404/01026 ,  C12Y121/03008 ,  C12N1/185

Abstract: The compositions and methods of the disclosure can be used to produce a cannabinoid in a host cell, such as a yeast cell. For example, the disclosure features host cells (e.g., yeast cells) modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an acyl activating enzyme (AAE), a tetraketide synthase (TKS), a cannabigerolic acid synthase (CBGaS), and/or an olivetolic acid cyclase (OAC).

公开(公告)号：US20220127620A1

公开(公告)日：2022-04-28

申请号：US17438810

申请日：2020-03-13

Applicant: Andrew P. KLEIN ,  Amyris, Inc.

Inventor： Christopher D. REEVES ,  Christopher J. PADDON ,  Victor F. HOLMES ,  Andrew P. KLEIN

IPC: C12N15/52 ,  C12N1/18 ,  C12N9/10 ,  C12N9/88 ,  C12P7/42

Abstract: Provide are modified host cells that are engineered to decrese expression of a product to undetectable levels in the presence of an exogenous agent, and increase expression of the product in the presence of another exogenous agent. The modified yeast strainshost cells do not express detectable levels of a precursor or substrate used to make the product. The product can be a cannabinoid or precursor thereof, and the substrate can be hexanoate. Also provided are methods for making a product using the modified host cells. The modified host cell can be a yeast strain, such as S. cerevisiae.

公开(公告)号：US20220282228A1

公开(公告)日：2022-09-08

申请号：US17608437

申请日：2020-05-01

Applicant: Amyris, Inc.

Inventor： Andrew P. KLEIN ,  Sean LUND ,  Gale WICHMANN ,  Irina KORYAKINA ,  Kyle HOGAN ,  Svetlana BORISOVA

IPC: C12N9/02 ,  C12P19/56

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of Ro.KAH. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides including rebaudioside A, rebaudioside D, and rebaudioside M.