公开(公告)号：US07572900B2

公开(公告)日：2009-08-11

申请号：US10479307

申请日：2002-05-30

申请人： Andreas Strasser ,  Hamsa Puthalakath ,  Andreas Villunger ,  Leigh Coultas ,  Jennifer Beaumont ,  Lorraine Ann O'Reilly ,  David Ching Siang Huang

发明人： Andreas Strasser ,  Hamsa Puthalakath ,  Andreas Villunger ,  Leigh Coultas ,  Jennifer Beaumont ,  Lorraine Ann O'Reilly ,  David Ching Siang Huang

IPC分类号： C07H21/04

CPC分类号： C07K14/4747 ,  A01K2217/05 ,  A61K38/00

摘要： The present invention relates generally to novel molecules capable of, inter alia, modulating apoptosis in mammalian cells and to genetic sequences encoding same. More particularly, the present invention relates to a novel member of the Bcl-2 family of proteins, referred to herein as “Bmf”, and to genetic sequences encoding same and to regulatory sequences such as a promoter sequence directing expression of Bmf. Bmf comprises a BH3 domain which facilitates interaction to pro-survival Bcl-2 family members thereby triggering apoptosis. Bmf is regarded, therefore, as a BH3-only molecule. The molecules of the present invention are useful, for example, in therapy, diagnosis, antibody generation and as a screening tool for therapeutic agents capable of modulating physiological cell death or survival and/or modulating cell cycle entry. The present invention further contemplates genetically modified animals in which one or both alleles of Bmf are mutated or partially or wholly deleted alone or in combination with a mutation in one or both alleles of another Bcl-2-type molecule such as but not limited to Bim. The genetically modified animals are useful inter alia in screening for agents which ameliorate the symptoms of diseases caused by defects in apoptosis or which specifically promote apoptosis of target cells.

摘要翻译： 本发明一般涉及能够特别调节哺乳动物细胞凋亡和编码其的遗传序列的新型分子。 更具体地，本发明涉及Bcl-2家族蛋白质的新成员，本文称为“Bmf”，以及编码它们的遗传序列以及调节序列，例如引导Bmf表达的启动子序列。 Bmf包含BH3结构域，其促进与生存Bcl-2家族成员的相互作用，从而引发细胞凋亡。 因此，Bmf被认为是仅BH3分子。 本发明的分子可用于例如治疗，诊断，抗体产生以及作为能够调节生理细胞死亡或生存和/或调节细胞周期进入的治疗剂的筛选工具。 本发明进一步考虑了遗传修饰的动物，其中Bmf的一个或两个等位基因单独突变或部分或全部缺失，或与另一个Bcl-2型分子的一个或两个等位基因中的突变组合，例如但不限于Bim 。 遗传修饰的动物尤其可用于筛选改善由凋亡缺陷引起的疾病症状或特异性促进靶细胞凋亡的药剂。

公开(公告)号：US20050064593A1

公开(公告)日：2005-03-24

申请号：US10479307

申请日：2002-05-30

申请人： Andreas Strasser ,  Hamsa Puthalakath ,  Andreas Villunger ,  Leigh Coultas ,  Jennifer Beaumont ,  Lorraine O'reilly ,  David Huang

发明人： Andreas Strasser ,  Hamsa Puthalakath ,  Andreas Villunger ,  Leigh Coultas ,  Jennifer Beaumont ,  Lorraine O'reilly ,  David Huang

IPC分类号： A01K67/027 ,  A61K38/00 ,  A61K45/00 ,  A61P31/12 ,  A61P35/00 ,  A61P37/00 ,  A61P43/00 ,  C07K14/47 ,  C07K16/18 ,  C12N5/10 ,  C12N15/09 ,  C12N15/11 ,  C12N15/12 ,  C12P21/08 ,  G01N33/53 ,  A61K48/00 ,  C07H21/04

CPC分类号： C07K14/4747 ,  A01K2217/05 ,  A61K38/00

摘要： The present invention relates generally to novel molecules capable of, inter alia, modulating apoptosis in mammalian cells and to genetic sequences encoding same. More particularly, the present invention relates to a novel member of the Bcl-2 family of proteins, referred to herein as “Bmf”, and to genetic sequences encoding same and to regulatory sequences such as a promoter sequence directing expression of Bmf. Bmf comprises a BH3 domain which facilitates interaction to pro-survival Bcl-2 family members thereby triggering apoptosis. Bmf is regarded, therefore, as a BH3-only molecule. The molecules of the present invention are useful, for example, in therapy, diagnosis, antibody generation and as a screening tool for therapeutic agents capable of modulating physiological cell death or survival and/or modulating cell cycle entry. The present invention further contemplates genetically modified animals in which one or both alleles of Bmf are mutated or partially or wholly deleted alone or in combination with a mutation in one or both alleles of another Bcl-2-type molecule such as but not limited to Bim. The genetically modified animals are useful inter alia in screening for agents which ameliorate the symptoms of diseases caused by defects in apoptosis or which specifically promote apoptosis of target cells.

摘要翻译： 本发明一般涉及能够特别调节哺乳动物细胞凋亡和编码其的遗传序列的新型分子。 更具体地，本发明涉及Bcl-2家族蛋白质的新成员，本文称为“Bmf”，以及编码它们的遗传序列以及调节序列，例如引导Bmf表达的启动子序列。 Bmf包含BH3结构域，其促进与生存Bcl-2家族成员的相互作用，从而引发细胞凋亡。 因此，Bmf被认为是仅BH3分子。 本发明的分子可用于例如治疗，诊断，抗体产生以及作为能够调节生理细胞死亡或生存和/或调节细胞周期进入的治疗剂的筛选工具。 本发明进一步考虑了遗传修饰的动物，其中Bmf的一个或两个等位基因单独突变或部分或全部缺失，或与另一个Bcl-2型分子的一个或两个等位基因中的突变组合，例如但不限于Bim 。 遗传修饰的动物尤其可用于筛选改善由凋亡缺陷引起的疾病症状或特异性促进靶细胞凋亡的药剂。

公开(公告)号：US07064193B1

公开(公告)日：2006-06-20

申请号：US09508832

申请日：1998-09-17

申请人： Suzanne Cory ,  Jerry Adams ,  David C. S. Huang ,  Liam O'Connor ,  Andreas Strasser ,  Hamsa Puthalakath ,  Lorraine O'Reilly

发明人： Suzanne Cory ,  Jerry Adams ,  David C. S. Huang ,  Liam O'Connor ,  Andreas Strasser ,  Hamsa Puthalakath ,  Lorraine O'Reilly

IPC分类号： C12N15/11 ,  C07K14/00

CPC分类号： C07K14/4747 ,  A61K38/00

摘要： The present invention relates generally to novel molecules capable of, inter alia, modulating apoptosis in mammalian cells and to genetic sequences encoding same. More particularly, the present invention relates to a novel member of the Bcl-2 family of proteins, referred to herein as “Bim”, and to genetic sequences encoding same. The molecules of the present invention are useful, for example, in therapy, diagnosis, antibody generation and as a screening tool for therapeutic agents capable of modulating physiological cell death or survival and/or modulating cell cycle entry.

公开(公告)号：US07582439B2

公开(公告)日：2009-09-01

申请号：US11409944

申请日：2006-04-24

申请人： Suzanne Cory ,  Jerry Adams ,  David C. S. Huang ,  Liam O'Connor ,  Andreas Strasser ,  Hamsa Puthalakath ,  Lorraine O'Reilly

发明人： Suzanne Cory ,  Jerry Adams ,  David C. S. Huang ,  Liam O'Connor ,  Andreas Strasser ,  Hamsa Puthalakath ,  Lorraine O'Reilly

IPC分类号： G01N33/536

CPC分类号： C07K14/4747 ,  A61K38/00

摘要： The present invention relates generally to novel molecules capable of, inter alia, modulating apoptosis in mammalian cells and to genetic sequences encoding same. More particularly, the present invention relates to a novel member of the Bcl-2 family of proteins, referred to herein as “Bim”, and to genetic sequences encoding same. The molecules of the present invention are useful, for example, in therapy, diagnosis, antibody generation and as a screening tool for therapeutic agents capable of modulating physiological cell death or survival and/or modulating cell cycle entry.

摘要翻译： 本发明一般涉及能够特别调节哺乳动物细胞凋亡和编码其的遗传序列的新型分子。 更具体地，本发明涉及Bcl-2家族蛋白质的新成员，本文称为“Bim”，以及编码它们的遗传序列。 本发明的分子可用于例如治疗，诊断，抗体产生以及作为能够调节生理细胞死亡或生存和/或调节细胞周期进入的治疗剂的筛选工具。

公开(公告)号：US20060183687A1

公开(公告)日：2006-08-17

申请号：US11409944

申请日：2006-04-24

申请人： Suzanne Cory ,  Jerry Adams ,  David Huang ,  Liam O'Connor ,  Andreas Strasser ,  Hamsa Puthalakath ,  Lorraine O'Reilly

发明人： Suzanne Cory ,  Jerry Adams ,  David Huang ,  Liam O'Connor ,  Andreas Strasser ,  Hamsa Puthalakath ,  Lorraine O'Reilly

IPC分类号： A61K38/54 ,  C07H21/04 ,  C12P21/06 ,  C12N9/99

CPC分类号： C07K14/4747 ,  A61K38/00

摘要： The present invention relates generally to novel molecules capable of, inter alia, modulating apoptosis in mammalian cells and to genetic sequences encoding same. More particularly, the present invention relates to a novel member of the Bcl-2 family of proteins, referred to herein as “Bim”, and to genetic sequences encoding same. The molecules of the present invention are useful, for example, in therapy, diagnosis, antibody generation and as a screening tool for therapeutic agents capable of modulating physiological cell death or survival and/or modulating cell cycle entry.

摘要翻译： 本发明一般涉及能够特别调节哺乳动物细胞凋亡和编码其的遗传序列的新型分子。 更具体地，本发明涉及Bcl-2家族蛋白质的新成员，本文称为“Bim”，以及编码它们的遗传序列。 本发明的分子可用于例如治疗，诊断，抗体产生以及作为能够调节生理细胞死亡或生存和/或调节细胞周期进入的治疗剂的筛选工具。