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公开(公告)号：US20060265781A1
公开(公告)日：2006-11-23
申请号：US11433097
申请日：2006-05-12
CPC分类号： C12N15/8222
摘要： The present invention is related to a family of novel spatiotemporally active Rubisco promoters (SEQ ID NO: 1, 2, 3) obtainable from light grown Brassica seedlings. Furthermore the invention is related to transgene expression in specific plant organs or at specific stages of plant development. DNA constructs and expression cassettes comprising at least one of the promoter sequences functionally fused in frame with genes encoding desired gene products are disclosed. Seeds from transformed homologous and heterologous plants and from subsequent generation of the transformed plants are collected and used for efficient production of desired gene products, especially in contained conditions.
摘要翻译： 本发明涉及可从光生芸苔属幼苗获得的新型时空活性Rubisco启动子家族（SEQ ID NO：1,2,3）。 此外，本发明涉及特定植物器官中或在植物发育的特定阶段的转基因表达。 公开了包含与编码所需基因产物的基因功能性融合的至少一个启动子序列的DNA构建体和表达盒。 收集来自转化的同源和异源植物和随后产生的转化植物的种子，并用于有效生产所需基因产物，特别是在包含的条件下。
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公开(公告)号：US07115733B2
公开(公告)日：2006-10-03
申请号：US10884283
申请日：2004-07-02
CPC分类号： C12N15/8222
摘要： The present invention is related to a family of novel spatiotemporally active Rubisco promoters (SEQ ID NO: 1, 2, 3) obtainable from light grown Brassica seedlings. Furthermore the invention is related to transgene expression in specific plant organs or at specific stages of plant development. DNA constructs and expression cassettes comprising at least one of the promoter sequences functionally fused in frame with genes encoding desired gene products are disclosed. Seeds from transformed homologous and herterologous plants and from subsequent generation of the transformed plants are collected and used for efficient production of desired gene products, especially in contained conditions.
摘要翻译： 本发明涉及可从光生芸苔属幼苗获得的新型时空活性Rubisco启动子家族（SEQ ID NO：1,2,3）。 此外，本发明涉及特定植物器官中或在植物发育的特定阶段的转基因表达。 公开了包含与编码所需基因产物的基因功能性融合的至少一个启动子序列的DNA构建体和表达盒。 收集来自转化的同源和异源植物以及随后产生转化植物的种子，并用于有效生产所需基因产物，特别是在含有条件下。
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公开(公告)号：US20050241022A1
公开(公告)日：2005-10-27
申请号：US10884283
申请日：2004-07-02
CPC分类号： C12N15/8222
摘要： The present invention is related to a family of novel spatiotemporally active Rubisco promoters (SEQ ID NO: 1, 2, 3) obtainable from light grown Brassica seedlings. Furthermore the invention is related to transgene expression in specific plant organs or at specific stages of plant development. DNA constructs and expression cassettes comprising at least one of the promoter sequences functionally fused in frame with genes encoding desired gene products are disclosed. Seeds from transformed homologous and herterologous plants and from subsequent generation of the transformed plants are collected and used for efficient production of desired gene products, especially in contained conditions.
摘要翻译： 本发明涉及可从光生芸苔属幼苗获得的新型时空活性Rubisco启动子家族（SEQ ID NO：1,2,3）。 此外，本发明涉及特定植物器官中或在植物发育的特定阶段的转基因表达。 公开了包含与编码所需基因产物的基因功能性融合的至少一个启动子序列的DNA构建体和表达盒。 收集来自转化的同源和异源植物以及随后产生转化植物的种子，并用于有效生产所需基因产物，特别是在含有条件下。
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4.发明申请Process for converting storage reserves of dicotyledonous seeds into compositions comprising one or more gene products 失效将双子叶种子的储存储备转化成包含一种或多种基因产物的组合物的方法公开(公告)号：US20080222751A1
公开(公告)日：2008-09-11
申请号：US11975198
申请日：2007-10-18
IPC分类号： C12N15/52
CPC分类号： C12N15/8222
摘要： The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicotyledonous seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.
摘要翻译： 本发明涉及一种基于源槽原理的方法，用于从破碎或未破碎的发芽双子叶种子产生感兴趣的产物，其包含在发芽过程中诱导或可诱导的表达系统。 产物是包含一种或多种基因产物的种子衍生组合物。 或者，通过将组合物与底物接触而获得的是令人感兴趣的产品，其含有能够由种子衍生的组合物本身转化的物质，干燥的或下游加工的形式。
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5.发明授权Process for converting storage reserves of dicotyledonous seeds into compositions comprising one or more gene products 失效将双子叶种子的储存储备转化成包含一种或多种基因产物的组合物的方法公开(公告)号：US07728192B2
公开(公告)日：2010-06-01
申请号：US11975198
申请日：2007-10-18
IPC分类号： C12N15/82
CPC分类号： C12N15/8222
摘要： The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicotyledonous seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.
摘要翻译： 本发明涉及一种基于源槽原理的方法，用于从破碎或未破碎的发芽双子叶种子产生感兴趣的产物，其包含在发芽过程中诱导或可诱导的表达系统。 产物是包含一种或多种基因产物的种子衍生组合物。 或者，通过将组合物与底物接触而获得的是令人感兴趣的产品，其含有能够由种子衍生的组合物本身转化的物质，干燥的或下游加工的形式。
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公开(公告)号：US20050039229A1
公开(公告)日：2005-02-17
申请号：US10892513
申请日：2004-07-15
申请人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
发明人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
CPC分类号： C12N15/8265 , A01H1/02 , A01H1/04 , C12N15/8213 , C12N15/8241 , C12N15/8251 , C12N15/8261 , C12N15/8263 , C12N15/8267 , C12N15/8287 , C12N15/829 , Y02A40/146
摘要： An improved double Recoverable Block of Function (RBF) system for transgene containment with enhanced reliability is disclosed. The system includes two blocking constructs (BCs) at the opposite sides of a transgene of interest (TGI). The system comprises means for recovery of the function blocked by expression of the blocking genes of the BCs. The invention is also related to a kit comprising a cloning vector having the BCs and TGI or alternatively a place for TGI to be inserted in. Segregating (two-insert) and inducible (one-insert) types of double RBF are disclosed. The two BCs are either similar or different in their DNA and RNA structures. The two BCs may encode same protein but promoters driving the blocking genes are different and/or the coding sequence of the blocking genes is modified by codon preference change. A system comprising two BCs having different blocking genes is also disclosed. The system may consist of one or two Recovering constructs (RC), but also a system recovering without an RC is disclosed. Whether the system includes an RC or not, the recovery is induced by an external intervention that is controlled by human being.
摘要翻译： 公开了一种改进的双重可恢复功能块（RBF）系统，用于提高可靠性的转基因遏制。 该系统在感兴趣的转基因（TGI）的相对侧包括两个阻断构建体（BC）。 该系统包括用于恢复由BC的阻断基因的表达阻断的功能的装置。 本发明还涉及一种试剂盒，其包含具有BC和TGI的克隆载体或替代地待插入的TGI的位置。公开了分离（双插入）和可诱导（单插入）类型的双RBF。 两个BC在其DNA和RNA结构中是相似的或不同的。 两个BC可以编码相同的蛋白质，但是驱动阻断基因的启动子是不同的，和/或阻断基因的编码序列被密码子偏好变化修饰。 还公开了包含具有不同阻断基因的两个BC的系统。 该系统可以由一个或两个恢复构造（RC）组成，而且还公开了没有RC的系统恢复。 无论系统是否包括RC，恢复是由受人控制的外部干预引起的。
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公开(公告)号：US07238853B2
公开(公告)日：2007-07-03
申请号：US10644664
申请日：2003-08-20
申请人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
发明人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
CPC分类号： A01H1/02 , A01H1/04 , C12N15/8213 , C12N15/8241 , C12N15/8251 , C12N15/8261 , C12N15/8263 , C12N15/8265 , C12N15/8287 , Y02A40/146
摘要： The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking construct (BC) that is inserted fully or partially into an intron of a transgene of interest (TGI). The TGI encodes desired gene products, such as heterologous or homologous proteins, peptides or other useful products. The expression of the BC leads to block of at least one molecular or physiological function that is essential for development or reproduction of the transgenic plant. Thereby the BC expression leads to death or incapacity of sexual reproduction of the plant. Moreover, the mechanism comprises an externally applicable recovering tool to recover the functions blocked by the BC. The recovering tool may be a recovering construct (RC).
摘要翻译： 本发明提供了在性繁殖转基因植物中的基因遏制的分子机制。 该机制通过包含完全或部分插入感兴趣的转基因（TGI）的内含子的阻断构建体（BC）的分子构建体来实现。 TGI编码所需的基因产物，例如异源或同源蛋白质，肽或其它有用的产物。 BC的表达导致对转基因植物的发育或繁殖至关重要的至少一种分子或生理功能的阻断。 因此，BC表达导致植物的有性繁殖的死亡或丧失工作能力。 此外，该机制包括一个外部应用的恢复工具，以恢复由BC封锁的功能。 恢复工具可以是恢复构造（RC）。
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公开(公告)号：US07790950B2
公开(公告)日：2010-09-07
申请号：US11632397
申请日：2005-07-05
申请人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
发明人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
CPC分类号： C12N15/8265 , A01H1/02 , A01H1/04 , C12N15/8213 , C12N15/8241 , C12N15/8251 , C12N15/8261 , C12N15/8263 , C12N15/8267 , C12N15/8287 , C12N15/829 , Y02A40/146
摘要： The present invention is related to a recoverable block of function (RBF) system for controlling transgene escape and transgene containment in plants. The RBF-system comprises at least one blocking construct (BC), wherein the BC comprises as the blocking gene a nucleotide sequence or crtB gene encoding phytoene synthase, which is used alone or in combination with other BCs. The crtB gene expresses under the control of a germination specific promoter or a promoter constitutively repressed by a Recovering construct (RC) and blocks the germination of a transgenic seed. The blocked germination is recoverable by a user controlled intervention including addition of an effective amount of gibberellin and sugar and may involve so called recovering constructs (RCs), wherein the recovering gene may encode ent-copalyl diphosphate synthase or ent-kaurene synthase.
摘要翻译： 本发明涉及用于控制植物中转基因逃逸和转基因遏制的可恢复功能块（RBF）系统。 RBF系统包含至少一个阻断构建体（BC），其中BC包含编码芸苔属合酶的核苷酸序列或crtB基因作为阻断基因，其单独使用或与其它BC组合使用。 crtB基因在由发芽特异性启动子或由恢复构建体（RC）组成型抑制的启动子的控制下表达，并阻断转基因种子的发芽。 封闭的萌发可以通过用户控制的干预来恢复，包括加入有效量的赤霉素和糖，并且可以涉及所谓的回收构建体（RC），其中回收基因可以编码内酰基二磷酸合成酶或ent-kaurene合酶。
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公开(公告)号：US07495148B2
公开(公告)日：2009-02-24
申请号：US10892513
申请日：2004-07-15
申请人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
发明人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
CPC分类号： C12N15/8265 , A01H1/02 , A01H1/04 , C12N15/8213 , C12N15/8241 , C12N15/8251 , C12N15/8261 , C12N15/8263 , C12N15/8267 , C12N15/8287 , C12N15/829 , Y02A40/146
摘要： An improved double Recoverable Block of Function (RBF) system for transgene containment with enhanced reliability is disclosed. The system includes two blocking constructs (BCs) at the opposite sides of a transgene of interest (TGI). The system comprises means for recovery of the function blocked by expression of the blocking genes of the BCs. The invention is also related to a kit comprising a cloning vector having the BCs and TGI or alternatively a place for TGI to be inserted in. Segregating (two-insert) and inducible (one-insert) types of double RBF are disclosed. The two BCs are either similar or different in their DNA and RNA structures. The two BCs may encode same protein but promoters driving the blocking genes are different and/or the coding sequence of the blocking genes is modified by codon preference change. A system comprising two BCs having different blocking genes is also disclosed. The system may consist of one or two Recovering constructs (RC), but also a system recovering without an RC is disclosed. Whether the system includes an RC or not, the recovery is induced by an external intervention that is controlled by human being.
摘要翻译： 公开了一种改进的双重可恢复功能块（RBF）系统，用于提高可靠性的转基因遏制。 该系统在感兴趣的转基因（TGI）的相对侧包括两个阻断构建体（BC）。 该系统包括用于恢复由BC的阻断基因的表达阻断的功能的装置。 本发明还涉及一种试剂盒，其包含具有BC和TGI的克隆载体或替代地待插入的TGI的位置。公开了分离（双插入）和可诱导（单插入）类型的双RBF。 两个BC在其DNA和RNA结构中是相似的或不同的。 两个BC可以编码相同的蛋白质，但是驱动阻断基因的启动子是不同的，和/或阻断基因的编码序列被密码子偏好变化修饰。 还公开了包含具有不同阻断基因的两个BC的系统。 该系统可以由一个或两个恢复构造（RC）组成，而且还公开了没有RC的系统恢复。 无论系统是否包括RC，恢复是由受人控制的外部干预引起的。
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公开(公告)号：US20080085996A1
公开(公告)日：2008-04-10
申请号：US11632397
申请日：2005-05-07
申请人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
发明人： Viktor Kuvshinov , Kimmo Koivu , Anne Kanerva , Andrei Anissimov
IPC分类号： C07H21/04
CPC分类号： C12N15/8265 , A01H1/02 , A01H1/04 , C12N15/8213 , C12N15/8241 , C12N15/8251 , C12N15/8261 , C12N15/8263 , C12N15/8267 , C12N15/8287 , C12N15/829 , Y02A40/146
摘要： The present invention is related to a recoverable block of function (RBF) system for controlling transgene escape and transgene containment in plants. The RBF-system comprises at least one blocking construct (BC), wherein the BC comprises as the blocking gene a nucleotide sequence or crtB gene encoding phytoene synthase, which is used alone or in combination with other BCs. The crtB gene expresses under the control of a germination specific promoter or a promoter constitutively repressed by a Recovering construct (RC) and blocks the germination of a transgenic seed. The blocked germination is recoverable by a user controlled intervention including addition of an effective amount of gibberellin and sugar and may involve so called recovering constructs (RCs), wherein the recovering gene may encode ent-copalyl diphosphate synthase or ent-kaurene synthase.
摘要翻译： 本发明涉及用于控制植物中转基因逃逸和转基因遏制的可恢复功能块（RBF）系统。 RBF系统包含至少一个阻断构建体（BC），其中BC包含编码芸苔属合酶的核苷酸序列或crtB基因作为阻断基因，其单独使用或与其它BC组合使用。 crtB基因在由发芽特异性启动子或由恢复构建体（RC）组成型抑制的启动子的控制下表达，并阻断转基因种子的发芽。 封闭的萌发可以通过用户控制的干预来恢复，包括加入有效量的赤霉素和糖，并且可以涉及所谓的回收构建体（RC），其中回收基因可以编码内酰基二磷酸合成酶或ent-kaurene合酶。
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