公开(公告)号：US07731826B2

公开(公告)日：2010-06-08

申请号：US11839793

申请日：2007-08-16

Applicant: Andrew D. Hibbs ,  Eric A. Duff

Inventor： Andrew D. Hibbs ,  Eric A. Duff

IPC: G01N27/26 ,  G01N27/28

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  C12Q2523/303 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: An electrolytic sensing system for measuring a blocking signal allows for controlled translocation of a molecule, such as DNA, through a fluid channel. A substantially constant electric field supplied by a DC source is applied across the fluid channel and induces translocation of the molecule within the system. An oscillating electric parameter (e.g. current or voltage) supplied by an AC source is also applied across the fluid channel as a means for measuring a blocking signal. The substantially constant electric field can be altered to provide more detailed control of the molecule and, optionally, run a select portion of the molecule through the channel multiple times to provide numerous signal readings. A temperature control stage cools the system, providing further control of molecule translocation. A modified or non-modified protein pore may be utilized in the fluid channel. The system allows for long DNA strands to be sequenced quickly without amplification.

Abstract translation: 用于测量阻塞信号的电解感测系统允许分子（例如DNA）通过流体通道的受控易位。 由DC源提供的基本上恒定的电场跨越流体通道施加并且诱导分子在系统内的易位。 由AC源提供的振荡电参数（例如电流或电压）也作为测量阻塞信号的装置跨越流体通道施加。 可以改变基本恒定的电场以提供对分子的更详细的控制，并且可选地，通过该通道多次运行分子的选择部分以提供大量的信号读数。 温度控制阶段冷却系统，进一步控制分子易位。 在流体通道中可以使用经修饰或未修饰的蛋白质孔。 该系统允许长DNA序列快速测序而无扩增。

公开(公告)号：US20080041733A1

公开(公告)日：2008-02-21

申请号：US11839793

申请日：2007-08-16

Applicant: Andrew D. Hibbs ,  Eric A. Duff

Inventor： Andrew D. Hibbs ,  Eric A. Duff

IPC: G01N27/26 ,  G01N27/28

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  C12Q2523/303 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: An electrolytic sensing system for measuring a blocking signal allows for controlled translocation of a molecule, such as DNA, through a fluid channel. A substantially constant electric field supplied by a DC source is applied across the fluid channel and induces translocation of the molecule within the system. An oscillating electric parameter (e.g. current or voltage) supplied by an AC source is also applied across the fluid channel as a means for measuring a blocking signal. The substantially constant electric field can be altered to provide more detailed control of the molecule and, optionally, run a select portion of the molecule through the channel multiple times to provide numerous signal readings. A temperature control stage cools the system, providing further control of molecule translocation. A modified or non-modified protein pore may be utilized in the fluid channel. The system allows for long DNA strands to be sequenced quickly without amplification.

Abstract translation: 用于测量阻塞信号的电解感测系统允许分子（例如DNA）通过流体通道的受控易位。 由DC源提供的基本上恒定的电场跨越流体通道施加并且诱导分子在系统内的易位。 由AC源提供的振荡电参数（例如电流或电压）也作为测量阻塞信号的装置跨越流体通道施加。 可以改变基本恒定的电场以提供对分子的更详细的控制，并且可选地，通过该通道多次运行分子的选择部分以提供大量的信号读数。 温度控制阶段冷却系统，进一步控制分子易位。 在流体通道中可以使用经修饰或未修饰的蛋白质孔。 该系统允许长DNA序列快速测序而无扩增。

公开(公告)号：US5500886A

公开(公告)日：1996-03-19

申请号：US224634

申请日：1994-04-06

Applicant: Eric A. Duff

Inventor： Eric A. Duff

IPC: G01T1/29 ,  G01D18/00

CPC classification number: G01T1/29

Abstract: A calibration system having a radiation source that generates the beam of radiation along the angular directions .theta., .phi.. A first reference element which is separated from the radiation source by a distance of approximately Z.sub.1 is then exposed to the beam of radiation. A second reference element is also present which is separated from the first reference element by a predetermined distance H and is exposed to the beam of radiation. An image detector produces images of the first and second reference elements, wherein the images are separated from each other by a distance R and angle .phi.. The device further includes an angle measurement device that calculates .theta. from the values of Z.sub.1 and H. A position measurement device having first and second sources of radiation that generate respective first and second beams of radiation directed at angles .theta..sub.1, .phi..sub.1, and .theta..sub.2, .phi..sub.2 respectively, toward an object of interest. An image detector is provided for producing 1) a first image of the object of interest formed by the first beam of radiation and 2) a second image of the object of interest formed by the second beam of radiation and that is separated from the first image by a distance L. A coordinate device then determines the coordinate of the object of interest from the values of .theta..sub.1, .theta..sub.2 and L.

Abstract translation: 一种具有辐射源的校准系统，该辐射源沿角度方向θ，phi产生辐射束。 然后将与辐射源分离大约Z1的距离的第一参考元件暴露于辐射束。 还存在与第一参考元件分开预定距离H并且暴露于辐射束的第二参考元件。 图像检测器产生第一和第二参考元件的图像，其中图像彼此分开距离R和角度phi。 该装置还包括角度测量装置，其从Z1和H的值计算θ。具有第一和第二辐射源的位置测量装置，其产生相应的第一和第二辐射光束，其以角度θ1，phi 1和θ 2，phi 2分别朝向感兴趣的对象。 提供图像检测器，用于产生1）由第一辐射束形成的感兴趣对象的第一图像和2）由第二辐射束形成并与第一图像分离的感兴趣对象的第二图像 然后坐标装置从θ1，θ2和L的值确定感兴趣对象的坐标。