公开(公告)号：US06511824B1

公开(公告)日：2003-01-28

申请号：US09362842

申请日：1999-07-28

申请人： Andrew Roy Buchman ,  Christian Burks ,  Helen Louise Francis-Lang ,  Lucile A. Gillett ,  Jonathan Cagampang Heller ,  Casey Casimir Kopczynski ,  Jonathan Scott Margolis ,  Darren Mark Platt ,  Bindu Priya Reddy ,  Candace Swimmer ,  John W. Winslow ,  Yuling Luo

发明人： Andrew Roy Buchman ,  Christian Burks ,  Helen Louise Francis-Lang ,  Lucile A. Gillett ,  Jonathan Cagampang Heller ,  Casey Casimir Kopczynski ,  Jonathan Scott Margolis ,  Darren Mark Platt ,  Bindu Priya Reddy ,  Candace Swimmer ,  John W. Winslow ,  Yuling Luo

IPC分类号： C12N1500

CPC分类号： C07K14/43581 ,  A01K67/0333 ,  A01K67/0336 ,  A01K67/0337 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2217/075 ,  C07K14/705 ,  C07K14/70571

摘要： Tandem pore domain weak inward rectifying K+ (TWIK) channel nucleic acids and proteins that have been isolated from Drosophila melanogaster and Leptinotarsa are described. The TWIK channel nucleic acids and proteins can be used to genetically modify metazoan invertebrate organisms, such as insects, coelomates, and pseudocoelomates, or cultured cells, resulting in TWIK channel expression or mis-expression. The genetically modified organisms or cells can be used in screening assays to identify candidate compounds which are potential pesticidal agents or therapeutics that interact with TWIK channel proteins. They can also be used in methods for studying TWIK channel activity and identifying other genes that modulate the function of, or interact with, the TWIK channel gene.

摘要翻译： 描述了从黑腹果蝇和Leptinotarsa分离的串联孔结构域弱内向整流K +（TWIK）通道核酸和蛋白质。 TWIK通道核酸和蛋白质可用于遗传修饰后生动物无脊椎动物生物体，如昆虫，群落和假细菌，或培养细胞，导致TWIK通道表达或错误表达。 遗传修饰的生物体或细胞可用于筛选测定以鉴定候选化合物，其是潜在的杀虫剂或与TWIK通道蛋白相互作用的治疗剂。 它们也可用于研究TWIK通道活性的方法，并鉴定调节TWIK通道基因功能或与之相互作用的其他基因。