公开(公告)号：US06703491B1

公开(公告)日：2004-03-09

申请号：US09270767

申请日：1999-03-17

申请人： Sheila Akiko Homburger ,  Allen James Ebens, Jr. ,  Catherine Sue Erickson ,  Helen Louise Francis-Lang ,  Jonathan Scott Margolis ,  Bindu Priya Reddy ,  David Andrew Ruddy ,  Andrew Roy Buchman

发明人： Sheila Akiko Homburger ,  Allen James Ebens, Jr. ,  Catherine Sue Erickson ,  Helen Louise Francis-Lang ,  Jonathan Scott Margolis ,  Bindu Priya Reddy ,  David Andrew Ruddy ,  Andrew Roy Buchman

IPC分类号： C07H2104

CPC分类号： A01K67/0333 ,  A01K67/0336 ,  A01K67/0337 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2217/075 ,  C07K14/70571

摘要： The present invention relates to Drosophila genes and methods for their use. The invention provides nucleotide sequences of Drosophila genes, amino acid sequences of the encoded proteins, and derivatives (e.g., fragments) and analogs thereof. The invention further relates to fragments (and derivatives and analogs thereof) of proteins which comprise one or more domains of a Drosophila protein. Antibodies to Drosophila proteins, and derivatives and analogs thereof, are also provided. Also provided herein are vectors and host cells comprising such nucleic acids. Methods of production of a Drosophila protein (e.g., by recombinant means), and derivatives and analogs thereof, are provided. Chimeric polypeptide molecules comprising polypeptides of the invention fused to heterologous polypeptide sequences are provided. Methods to identify the biological function of a Drosophila gene are provided, including various methods for the functional modification (e.g., overexpression, underexpression, mutation, knock-out) of one gene, or of two or more genes simultaneously. Methods to identify a Drosophila gene which modifies the function of, and/or functions in a downstream pathway from, another gene are provided. The invention further provides for use of Drosophila proteins as media additives or pesticides.

摘要翻译： 本发明涉及果蝇基因及其使用方法。 本发明提供了果蝇基因的核苷酸序列，编码的蛋白质的氨基酸序列及其衍生物（例如片段）及其类似物。 本发明还涉及包含果蝇蛋白的一个或多个结构域的蛋白质的片段（及其衍生物和类似物）。 还提供了针对果蝇蛋白的抗体及其衍生物和类似物。 本文还提供了包含这种核酸的载体和宿主细胞。 提供了果蝇蛋白的生产方法（例如通过重组方法）及其衍生物和类似物。 提供了包含与异源多肽序列融合的本发明多肽的嵌合多肽分子。 提供鉴定果蝇基因生物学功能的方法，包括一种基因或两种或多种基因同时进行功能修饰（如过表达，低表达，突变，敲除）的各种方法。 提供了鉴定另一基因的修饰下游途径的功能和/或功能的果蝇基因的方法。 本发明还提供了果蝇蛋白质作为媒介添加剂或杀虫剂的用途。

公开(公告)号：US06511824B1

公开(公告)日：2003-01-28

申请号：US09362842

申请日：1999-07-28

申请人： Andrew Roy Buchman ,  Christian Burks ,  Helen Louise Francis-Lang ,  Lucile A. Gillett ,  Jonathan Cagampang Heller ,  Casey Casimir Kopczynski ,  Jonathan Scott Margolis ,  Darren Mark Platt ,  Bindu Priya Reddy ,  Candace Swimmer ,  John W. Winslow ,  Yuling Luo

发明人： Andrew Roy Buchman ,  Christian Burks ,  Helen Louise Francis-Lang ,  Lucile A. Gillett ,  Jonathan Cagampang Heller ,  Casey Casimir Kopczynski ,  Jonathan Scott Margolis ,  Darren Mark Platt ,  Bindu Priya Reddy ,  Candace Swimmer ,  John W. Winslow ,  Yuling Luo

IPC分类号： C12N1500

CPC分类号： C07K14/43581 ,  A01K67/0333 ,  A01K67/0336 ,  A01K67/0337 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2217/075 ,  C07K14/705 ,  C07K14/70571

摘要： Tandem pore domain weak inward rectifying K+ (TWIK) channel nucleic acids and proteins that have been isolated from Drosophila melanogaster and Leptinotarsa are described. The TWIK channel nucleic acids and proteins can be used to genetically modify metazoan invertebrate organisms, such as insects, coelomates, and pseudocoelomates, or cultured cells, resulting in TWIK channel expression or mis-expression. The genetically modified organisms or cells can be used in screening assays to identify candidate compounds which are potential pesticidal agents or therapeutics that interact with TWIK channel proteins. They can also be used in methods for studying TWIK channel activity and identifying other genes that modulate the function of, or interact with, the TWIK channel gene.

摘要翻译： 描述了从黑腹果蝇和Leptinotarsa分离的串联孔结构域弱内向整流K +（TWIK）通道核酸和蛋白质。 TWIK通道核酸和蛋白质可用于遗传修饰后生动物无脊椎动物生物体，如昆虫，群落和假细菌，或培养细胞，导致TWIK通道表达或错误表达。 遗传修饰的生物体或细胞可用于筛选测定以鉴定候选化合物，其是潜在的杀虫剂或与TWIK通道蛋白相互作用的治疗剂。 它们也可用于研究TWIK通道活性的方法，并鉴定调节TWIK通道基因功能或与之相互作用的其他基因。