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1.发明授权Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use 有权无脊椎动物TWIK通道的核酸和多肽及其使用方法公开(公告)号：US06511824B1
公开(公告)日：2003-01-28
申请号：US09362842
申请日：1999-07-28
申请人： Andrew Roy Buchman , Christian Burks , Helen Louise Francis-Lang , Lucile A. Gillett , Jonathan Cagampang Heller , Casey Casimir Kopczynski , Jonathan Scott Margolis , Darren Mark Platt , Bindu Priya Reddy , Candace Swimmer , John W. Winslow , Yuling Luo
发明人： Andrew Roy Buchman , Christian Burks , Helen Louise Francis-Lang , Lucile A. Gillett , Jonathan Cagampang Heller , Casey Casimir Kopczynski , Jonathan Scott Margolis , Darren Mark Platt , Bindu Priya Reddy , Candace Swimmer , John W. Winslow , Yuling Luo
IPC分类号： C12N1500
CPC分类号： C07K14/43581 , A01K67/0333 , A01K67/0336 , A01K67/0337 , A01K67/0339 , A01K2217/05 , A01K2217/075 , C07K14/705 , C07K14/70571
摘要： Tandem pore domain weak inward rectifying K+ (TWIK) channel nucleic acids and proteins that have been isolated from Drosophila melanogaster and Leptinotarsa are described. The TWIK channel nucleic acids and proteins can be used to genetically modify metazoan invertebrate organisms, such as insects, coelomates, and pseudocoelomates, or cultured cells, resulting in TWIK channel expression or mis-expression. The genetically modified organisms or cells can be used in screening assays to identify candidate compounds which are potential pesticidal agents or therapeutics that interact with TWIK channel proteins. They can also be used in methods for studying TWIK channel activity and identifying other genes that modulate the function of, or interact with, the TWIK channel gene.
摘要翻译： 描述了从黑腹果蝇和Leptinotarsa分离的串联孔结构域弱内向整流K +（TWIK）通道核酸和蛋白质。 TWIK通道核酸和蛋白质可用于遗传修饰后生动物无脊椎动物生物体，如昆虫，群落和假细菌，或培养细胞，导致TWIK通道表达或错误表达。 遗传修饰的生物体或细胞可用于筛选测定以鉴定候选化合物，其是潜在的杀虫剂或与TWIK通道蛋白相互作用的治疗剂。 它们也可用于研究TWIK通道活性的方法，并鉴定调节TWIK通道基因功能或与之相互作用的其他基因。
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公开(公告)号：US08951726B2
公开(公告)日：2015-02-10
申请号：US12660524
申请日：2010-02-26
申请人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
发明人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20110105351A1
公开(公告)日：2011-05-05
申请号：US12804904
申请日：2010-07-29
申请人： Yuling Luo , Wen Yang
发明人： Yuling Luo , Wen Yang
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6834 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313 , C12Q2565/543 , C12Q2565/501 , C12Q2563/149 , C12Q2563/103
摘要： Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了在多重支链DNA测定中检测两种或多种核酸的方法。 通过在不同的，可识别的颗粒子集上或在空间可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US07803541B2
公开(公告)日：2010-09-28
申请号：US11433081
申请日：2006-05-11
申请人： Yuling Luo , Wen Yang
发明人： Yuling Luo , Wen Yang
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6834 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313 , C12Q2565/543 , C12Q2565/501 , C12Q2563/149 , C12Q2563/103
摘要： Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了在多重支链DNA测定中检测两种或多种核酸的方法。 通过在不同的，可识别的颗粒子集上或在空间可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US08632970B2
公开(公告)日：2014-01-21
申请号：US12930041
申请日：2010-12-23
申请人： Yuling Luo , Wen Yang
发明人： Yuling Luo , Wen Yang
CPC分类号： C12Q1/6874 , C12Q1/6832 , C12Q1/6834 , C12Q1/6837 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on different subsets of particles or at different selected positions on a spatially addressable solid support. Methods of capturing one or more long nucleic acids and methods of capturing one or more nucleic acid for sequencing are also provided. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了从单个样品同时捕获两种或更多种核酸的方法。 通过在不同的颗粒子集上或在空间上可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还提供了捕获一种或多种长核酸的方法和捕获一种或多种核酸进行测序的方法。 还描述了与方法相关的组合物，试剂盒和系统。
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6.发明申请RNASCOPE® HPV ASSAY FOR DETERMINING HPV STATUS IN HEAD AND NECK CANCERS AND CERVICAL LESIONS 审中-公开用于确定头颈部和颈部癌症和宫颈癌HPV状态的RNASCOPE®HPV测定公开(公告)号：US20120214152A1
公开(公告)日：2012-08-23
申请号：US13359706
申请日：2012-01-27
申请人： Xiao-Jun Ma , John Flanagan , Yuling Luo
发明人： Xiao-Jun Ma , John Flanagan , Yuling Luo
CPC分类号： C12Q1/708 , C12Q1/6841 , C12Q1/6886 , C12Q2600/112 , C12Q2600/118
摘要： The present invention provides a method and a kit for determining whether a head and neck cancer is HPV-related. In one embodiment, an RNAscope® HPV assay was designed to detect the presence of E6/E7 mRNA of certain high-risk HPV subtypes related to head and neck cancer. The present invention also provides a method and a kit for determining whether a cervical lesion is a benign lesion or a cervical intraepithethial neoplasm lesion. The present invention further provides a method for determining the progression of cervical intraepithethial neoplasm based on the spatial pattern and levels of the E6/E7 mRNA of certain high-risk HPV subtypes. The present invention also provides a method for determining the risk of developing cervical cancer in a human diagnosed with cervical intraepithethial neoplasm based on presence and absence of the certain subgroups of high-risk HPV subtypes.
摘要翻译： 本发明提供了一种用于确定头颈癌是否与HPV相关的方法和试剂盒。 在一个实施方案中，设计了RNA检测HPV检测来检测某些与头颈癌相关的高危HPV亚型的E6 / E7 mRNA的存在。 本发明还提供了一种用于确定子宫颈病变是否是良性病变或子宫颈上皮内肿瘤病变的方法和试剂盒。 本发明还提供了一种基于某些高风险HPV亚型的E6 / E7 mRNA的空间格局和水平来确定子宫颈上皮内肿瘤进展的方法。 本发明还提供了一种基于存在和不存在某些亚型的高风险HPV亚型的方法来确定诊断为宫颈上皮内瘤的人的宫颈癌发生风险的方法。
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公开(公告)号：US20110223606A1
公开(公告)日：2011-09-15
申请号：US13068727
申请日：2011-05-17
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solubilize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下从保存的临床样品中有效溶解核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US07968327B2
公开(公告)日：2011-06-28
申请号：US11893356
申请日：2007-08-14
申请人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
发明人： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
摘要： This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
摘要翻译： 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US20110059866A1
公开(公告)日：2011-03-10
申请号：US12660516
申请日：2010-02-26
申请人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
发明人： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC分类号： C40B40/06
CPC分类号： C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
摘要： Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
摘要翻译： 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
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10.发明申请Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations 审中-公开检测单个细胞中的核酸并鉴定来自大异质细胞群体的稀有细胞的方法公开(公告)号：US20090081688A1
公开(公告)日：2009-03-26
申请号：US12284163
申请日：2008-09-17
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q2537/143 , C12Q2537/125
摘要： Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.
摘要翻译： 提供了通过间接捕获标记到核酸来检测单个细胞中的多个核酸靶的方法。 还提供了通过归一化到参考核酸水平来测定核酸靶的相对水平的方法。 描述了通过检测核酸检测单个细胞，特别是来自大异质细胞群体的罕见细胞的方法。 还提供了相关的组合物，系统和试剂盒。
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