公开(公告)号：US20040096397A1

公开(公告)日：2004-05-20

申请号：US10602974

申请日：2003-06-24

Applicant: Applera Corporation

Inventor： Steven M. Menchen ,  Scott C. Benson ,  Joe Y.L. Lam ,  Weiguo Zhen ,  Daqing Sun ,  Barnett B. Rosenblum ,  Shaheer H. Khan ,  Meng Taing

IPC: A61K049/00 ,  C12Q001/68 ,  A61K031/4433 ,  A61K031/343 ,  C07D45/02 ,  C07D311/94 ,  C07D311/78

CPC classification number: C07D311/78 ,  C07D209/60 ,  C07D221/10 ,  C07D311/94 ,  C07D491/14 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C09B11/02 ,  C09B11/24

Abstract: Sulfonated diarylrhodamine compounds are useful as fluorescent labels of nucleosides, nucleotides, polynucleotides, and polypeptides. The compounds find particular application in the area of fluorescent nucleic acid analysis, e.g., automated DNA sequencing and fragment analysis, detection of probe hybridization in hybridization arrays, detection of nucleic acid amplification products, and the like.

Abstract translation: 磺化二芳基罗丹明化合物可用作核苷，核苷酸，多核苷酸和多肽的荧光标记。 该化合物在荧光核酸分析的领域中具体应用，例如自动DNA测序和片段分析，杂交阵列中探针杂交的检测，核酸扩增产物的检测等。

公开(公告)号：US20040229235A1

公开(公告)日：2004-11-18

申请号：US10716165

申请日：2003-11-18

Applicant: Applera Corporation

Inventor： Linda G. Lee ,  Meng C. Taing ,  Barnett B. Rosenblum

IPC: C12Q001/68 ,  C07H021/04 ,  C07D311/82

CPC classification number: C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C09B11/08 ,  C09B11/24 ,  G01N33/533

Abstract: Atropisomeric energy-transfer dye compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.

Abstract translation: 公开了异转移能量转移染料化合物。 各种分子生物学应用利用无异构呫吨荧光染料作为底物的标记，例如核苷酸，核苷，多核苷酸，多肽和碳水化合物。 方法包括DNA测序，DNA片段分析，PCR，SNP分析，寡核苷酸连接，扩增，minisequencing和引物延伸。

公开(公告)号：US20030191303A1

公开(公告)日：2003-10-09

申请号：US10371985

申请日：2003-02-20

Applicant: Applera Corporation

Inventor： Ravi S. Vinayak ,  Linda G. Lee ,  Khairuzzaman B. Mullah ,  Barnett B. Rosenblum

IPC: C07H021/04

CPC classification number: C07H21/00 ,  C07H19/10 ,  C07H19/20 ,  Y02P20/55

Abstract: Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure 1 where S is a solid-support, A is a cleavable linker, X is a moiety with three or more attachment sites, L is a label, Y is a nucleophile, i.e. O, NH, NR or S, and P1 is an acid cleavable protecting group are provided. The labelled solid-support is reacted in a cyclical fashion to synthesize a labelled oligonucleotide on a solid-support in the 5null to 3null direction, having the structure: 2 Labelled oligonucleotides are also synthesized by reacting: (i) a label reagent bearing functionality consisting of carboxylic acid, sulfonic acid, phosphonic acid, or phosphoric acid, (ii) an oligonucleotide on solid support with nucleophilic functionality, and (iii) a coupling reagent, whereby an ester, amide, thioester, sulfonamide, sulfonate, phosphonate, phosphoramidate, phosphorothioate, or phosphate bond is formed. The labelling reaction may be conducted at label sites including the 5null terminus, the 3null terminus, a nucleobase, an internucleotide linkage, a sugar, amino, sulfide, hydroxyl, and carboxyl.

Abstract translation: 方法和组合物直接在具有S是固体支持物的结构的固体支持物上标记寡核苷酸和类似物，A是可切割的连接体，X是具有三个或更多个连接位点的部分，L是标记，Y是一个 亲核试剂，即O，NH，NR或S，P1是可酸切割的保护基团。 标记的固体支持物以循环方式反应以在5'至3'方向的固体支持物上合成标记的寡核苷酸，具有以下结构：标记的寡核苷酸还通过以下方式合成：（i）标记试剂载体官能团 包括羧酸，磺酸，膦酸或磷酸，（ii）具有亲核官能团的固体支持物上的寡核苷酸，和（iii）偶联试剂，其中酯，酰胺，硫酯，磺酰胺，磺酸酯，膦酸酯，氨基磷酸酯 ，硫代磷酸酯或磷酸酯键。 标记反应可以在包括5'末端，3'末端，核碱基，核苷酸间键，糖，氨基，硫化物，羟基和羧基的标记位点进行。