公开(公告)号：US20040229235A1

公开(公告)日：2004-11-18

申请号：US10716165

申请日：2003-11-18

Applicant: Applera Corporation

Inventor： Linda G. Lee ,  Meng C. Taing ,  Barnett B. Rosenblum

IPC: C12Q001/68 ,  C07H021/04 ,  C07D311/82

CPC classification number: C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C09B11/08 ,  C09B11/24 ,  G01N33/533

Abstract: Atropisomeric energy-transfer dye compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension.

Abstract translation: 公开了异转移能量转移染料化合物。 各种分子生物学应用利用无异构呫吨荧光染料作为底物的标记，例如核苷酸，核苷，多核苷酸，多肽和碳水化合物。 方法包括DNA测序，DNA片段分析，PCR，SNP分析，寡核苷酸连接，扩增，minisequencing和引物延伸。