公开(公告)号：US20130065771A1

公开(公告)日：2013-03-14

申请号：US13199811

申请日：2011-09-09

申请人： Asha A. Oroskar ,  Anil R. Oroskar ,  John P. LaCava ,  Michael P. Rout

发明人： Asha A. Oroskar ,  Anil R. Oroskar ,  John P. LaCava ,  Michael P. Rout

IPC分类号： C40B30/04 ,  C40B60/12 ,  G01N30/02 ,  C40B60/08

CPC分类号： B01L3/50255 ,  B01L2200/0631 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N33/6803 ,  G01N35/00

摘要： This invention relates to a kit and a method for the collection and analysis of complex protein mixtures. More particularly, the invention relates to a kit comprising a single barrel filtration well or a multi-well filtration plate wherein each well comprises an upper filtration zone; a lower filtration zone; a conical flow director zone; and, an elution tip, wherein the upper filtration zone and the lower filtration zone are separated by a retainer ring disposed within the lower filtration zone. The upper filtration zone comprises an upper collection zone, a sponge zone, and a deep bed filtration zone; and, the lower filtration zone comprises the retainer ring, a supported hydrophilic membrane and a lower bed filtration media. When used with an array of selected buffer solutions, the multi-well filtration plate can provide accurate, automated, high-throughput protein analysis by affinity chromatography.

公开(公告)号：US09084994B2

公开(公告)日：2015-07-21

申请号：US13199811

申请日：2011-09-09

申请人： Asha A. Oroskar ,  John P. LaCava ,  Michael Paul Rout ,  Anil R. Oroskar

发明人： Asha A. Oroskar ,  John P. LaCava ,  Michael Paul Rout ,  Anil R. Oroskar

IPC分类号： G01N30/02 ,  B01L3/00 ,  G01N33/68 ,  G01N35/00

CPC分类号： B01L3/50255 ,  B01L2200/0631 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N33/6803 ,  G01N35/00

摘要： This invention relates to a kit and a method for the collection and analysis of complex protein mixtures. More particularly, the invention relates to a kit comprising a single barrel filtration well or a multi-well filtration plate wherein each well comprises an upper filtration zone; a lower filtration zone; a conical flow director zone; and, an elution tip, wherein the upper filtration zone and the lower filtration zone are separated by a retainer ring disposed within the lower filtration zone. The upper filtration zone comprises an upper collection zone, a sponge zone, and a deep bed filtration zone; and, the lower filtration zone comprises the retainer ring, a supported hydrophilic membrane and a lower bed filtration media. When used with an array of selected buffer solutions, the multi-well filtration plate can provide accurate, automated, high-throughput protein analysis by affinity chromatography.

摘要翻译： 本发明涉及一种用于收集和分析复合蛋白质混合物的试剂盒和方法。 更具体地说，本发明涉及包括单筒过滤井或多孔过滤板的试剂盒，其中每个孔包括上部过滤区; 一个较低的过滤区; 圆锥形导流器区; 以及洗脱头，其中上过滤区和下过滤区被设置在下过滤区内的保持环分开。 上部过滤区域包括上部收集区域，海绵区域和深层过滤区域; 并且，下部过滤区域包括保持环，支撑的亲水膜和下层过滤介质。 当与所选择的缓冲溶液阵列一起使用时，多孔过滤板可以通过亲和层析提供准确，自动化，高通量的蛋白质分析。