公开(公告)号：US20240084271A1

公开(公告)日：2024-03-14

申请号：US18262127

申请日：2021-03-21

申请人： ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD.

发明人： Hao Hong ,  James Gage ,  Yi Xiao ,  Na Zhang ,  Xuecheng Jiao ,  Rui Li ,  Yanqing Zhang ,  Yiming Yang

IPC分类号： C12N9/06 ,  C12N15/70 ,  C12P13/04

CPC分类号： C12N9/0016 ,  C12N15/70 ,  C12P13/04

摘要： Provided are an amino acid dehydrogenase mutant and use thereof. The amino acid sequence of the amino acid dehydrogenase mutant has a sequence as shown in SEQ ID NO: 1, and sites at which amino acid mutations occur include a K144G site.

公开(公告)号：US11407982B2

公开(公告)日：2022-08-09

申请号：US16756649

申请日：2017-11-06

申请人： Asymchem Life Science (Tianjin) Co., Ltd

发明人： Hao Hong ,  Gage James ,  Jiangping Lu ,  Xuecheng Jiao ,  Na Zhang ,  Rui Li ,  Kejian Zhang

IPC分类号： C12N9/06 ,  C12N9/10 ,  C12P13/00 ,  C12P17/12

摘要： Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.

公开(公告)号：US20210292717A1

公开(公告)日：2021-09-23

申请号：US17266461

申请日：2018-08-17

申请人： ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD

发明人： Hao Hong ,  Gage James ,  Jiangping Lu ,  Na Zhang ,  Xuecheng Jiao ,  Rui Li ,  Kejian Zhang ,  Yu Zhang ,  Yiming Yang

IPC分类号： C12N9/06 ,  C12N15/70 ,  C12P13/04

摘要： Provided is an amino acid dehydrogenase mutant. The amino acid sequence of the mutant is obtained by mutating the amino acid sequence shown in SEQ ID NO:1. The mutation includes at least one of the following mutation sites: 64th, 94th, 133rd, 137th, 148th, 168th, 173rd, 183 rd, 191st, 207th, 229th, 248th, 255th and 282nd sites; or the amino acid sequence of the amino acid dehydrogenase mutant is an amino acid sequence having the mutation sites in the mutated amino acid sequence and having a 80% or more homology with the mutated amino acid sequence. The mutant enzyme activity is more than 50 times higher than that of wild amino acid dehydrogenase, and the enzyme specificity is also correspondingly improved.

公开(公告)号：US11603521B2

公开(公告)日：2023-03-14

申请号：US17266461

申请日：2018-08-17

申请人： ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD

发明人： Hao Hong ,  Gage James ,  Jiangping Lu ,  Na Zhang ,  Xuecheng Jiao ,  Rui Li ,  Kejian Zhang ,  Yu Zhang ,  Yiming Yang

IPC分类号： C12N9/06 ,  C12N15/70 ,  C12P13/04 ,  C12R1/19

摘要： Provided is an amino acid dehydrogenase mutant. The amino acid sequence of the mutant is obtained by mutating the amino acid sequence shown in SEQ ID NO:1. The mutation includes at least one of the following mutation sites: 64th, 94th, 133rd, 137th, 148th, 168th, 173rd, 183 rd, 191st, 207th, 229th, 248th, 255th and 282nd sites; or the amino acid sequence of the amino acid dehydrogenase mutant is an amino acid sequence having the mutation sites in the mutated amino acid sequence and having a 80% or more homology with the mutated amino acid sequence. The mutant enzyme activity is more than 50 times higher than that of wild amino acid dehydrogenase, and the enzyme specificity is also correspondingly improved.