公开(公告)号：US08175369B2

公开(公告)日：2012-05-08

申请号：US12316497

申请日：2008-12-12

Applicant: Dihui Hong ,  Avrum I. Cohen

Inventor： Dihui Hong ,  Avrum I. Cohen

IPC: G06K9/00

CPC classification number: G06K9/0014 ,  G06K9/342 ,  G06K9/48

Abstract: Methods and systems for counting nuclei for cells in a cell-containing sample are disclosed, such as carried out on a computer. The methods comprise: receiving a raw image of the cell-containing sample; transforming the raw image into a segmented image comprising one or more nuclei clusters. The methods further comprise: for each of the one or more nuclei clusters, obtaining a convex hull of the nuclei cluster; locating any indentations on the nuclei cluster by comparing the nuclei cluster to the convex hull of the nuclei cluster; calculating a first nuclei count based on a tally of the indentations; and assigning the nuclei cluster to a cell among the plurality of cells. The methods further comprise: calculating a second nuclei count for each cell by totaling the first nuclei counts of its constituent nuclei clusters; and presenting a result based on the second nuclei count for at least one of the plurality of cells.

Abstract translation: 公开了用于计数含细胞样品中的细胞核的方法和系统，例如在计算机上进行的。 所述方法包括：接收含细胞样品的原始图像; 将原始图像转换成包括一个或多个核簇的分割图像。 所述方法还包括：对于所述一个或多个核簇中的每一个，获得所述核簇的凸包; 通过将核簇与核簇的凸包进行比较来确定核簇上的任何缺陷; 基于压痕的计数计算第一核计数; 以及将所述核簇分配给所述多个小区中的小区。 所述方法还包括：通过总计其组成核簇的第一核计数来计算每个细胞的第二核计数; 以及基于所述多个单元中的至少一个单元的第二核计数呈现结果。

公开(公告)号：US08687180B2

公开(公告)日：2014-04-01

申请号：US13491017

申请日：2012-06-07

Applicant: Avrum I. Cohen

Inventor： Avrum I. Cohen

IPC: G01B9/00 ,  G01M11/02

CPC classification number: G01M11/0228 ,  G01B9/04 ,  G02B21/006 ,  G02B21/245 ,  G06F19/10

Abstract: A system and method of determining a focal position for an objective positioned at a measurement location of a sample holder in a microscopy imaging system are provided. The objective is moved to a position relative to the sample holder that corresponds to a distance between the objective and the sample holder. The sample holder has a conditioned upper surface. A focusing light beam is projected onto the sample holder when the objective is located at the position, and the objective focuses the focusing light beam on the sample holder. A reflected light beam resulting from reflection of the focusing light beam off the conditioned upper surface is observed. The focal position for the objective is determined based on the reflected light beam such that the objective produces an in focus image of a microscopy sample when the objective is located at the focal position.

Abstract translation: 提供了一种用于确定位于显微镜成像系统中的样品架的测量位置处的物镜的焦点位置的系统和方法。 将物镜移动到相对于样品架的位置，该位置对应于物镜与样品架之间的距离。 样品架具有调节的上表面。 当物镜位于该位置时，聚焦光束投影到样品架上，并且物镜将聚焦光束聚焦在样品架上。 观察到由聚焦光束从调节的上表面反射而产生的反射光束。 基于反射光束确定目标的焦点位置，使得当物镜位于焦点位置时，物镜产生显微镜样本的聚焦图像。

公开(公告)号：US08564623B2

公开(公告)日：2013-10-22

申请号：US12636568

申请日：2009-12-11

Applicant: Avrum I. Cohen ,  Jerry M. Rubinow ,  Steven P. Boyd ,  Arad Shaiber

Inventor： Avrum I. Cohen ,  Jerry M. Rubinow ,  Steven P. Boyd ,  Arad Shaiber

IPC: G09G5/14

CPC classification number: G06T19/00 ,  G02B21/367 ,  G06T11/206 ,  G06T2200/24

Abstract: Methods, systems, and apparatus, including computer programs encoded on a computer storage medium, for implementing an integrated data visualization interface for multi-dimensional microscopy image and measurement data are disclosed. The integrated data visualization interface utilizes the known dimensional relational relationships between the images to automatically provide synchronized and coordinated visualization and manipulations across multiple related images and measurement data in multiple dataset viewer components (e.g., image grids, browsable image strips, tables, and graphs). Other features of the dataset viewer components are also disclosed.

Abstract translation: 公开了包括用于实现用于多维显微镜图像和测量数据的集成数据可视化界面的计算机存储介质上编码的计算机程序的方法，系统和装置。 集成数据可视化界面利用图像之间的已知尺寸关系关系自动提供多个数据集查看器组件（例如，图像网格，可浏览的图像条，表格和图形）中的多个相关图像和测量数据的同步和协调的可视化和操纵。 。 还公开了数据集查看器组件的其他特征。

公开(公告)号：US20110141103A1

公开(公告)日：2011-06-16

申请号：US12636568

申请日：2009-12-11

Applicant: Avrum I. Cohen ,  Jerry M. Rubinow ,  Steven P. Boyd ,  Arad Shaiber

Inventor： Avrum I. Cohen ,  Jerry M. Rubinow ,  Steven P. Boyd ,  Arad Shaiber

IPC: G06T15/00

CPC classification number: G06T19/00 ,  G02B21/367 ,  G06T11/206 ,  G06T2200/24

Abstract: Methods, systems, and apparatus, including computer programs encoded on a computer storage medium, for implementing an integrated data visualization interface for multi-dimensional microscopy image and measurement data are disclosed. The integrated data visualization interface utilizes the known dimensional relational relationships between the images to automatically provide synchronized and coordinated visualization and manipulations across multiple related images and measurement data in multiple dataset viewer components (e.g., image grids, browsable image strips, tables, and graphs). Other features of the dataset viewer components are also disclosed.

Abstract translation: 公开了包括用于实现用于多维显微镜图像和测量数据的集成数据可视化界面的计算机存储介质上编码的计算机程序的方法，系统和装置。 集成数据可视化界面利用图像之间的已知尺寸关系关系自动提供多个数据集查看器组件（例如，图像网格，可浏览的图像条，表格和图形）中的多个相关图像和测量数据的同步和协调的可视化和操纵。 。 还公开了数据集查看器组件的其他特征。

公开(公告)号：US20100150423A1

公开(公告)日：2010-06-17

申请号：US12316497

申请日：2008-12-12

Applicant: Dihui Hong ,  Avrum I. Cohen

Inventor： Dihui Hong ,  Avrum I. Cohen

IPC: G06K9/00

CPC classification number: G06K9/0014 ,  G06K9/342 ,  G06K9/48

Abstract: Methods and systems for counting nuclei for cells in a cell-containing sample are disclosed, such as carried out on a computer. The methods comprise: receiving a raw image of the cell-containing sample; transforming the raw image into a segmented image comprising one or more nuclei clusters. The methods further comprise: for each of the one or more nuclei clusters, obtaining a convex hull of the nuclei cluster; locating any indentations on the nuclei cluster by comparing the nuclei cluster to the convex hull of the nuclei cluster; calculating a first nuclei count based on a tally of the indentations; and assigning the nuclei cluster to a cell among the plurality of cells. The methods further comprise: calculating a second nuclei count for each cell by totaling the first nuclei counts of its constituent nuclei clusters; and presenting a result based on the second nuclei count for at least one of the plurality of cells.

Abstract translation: 公开了用于计数含细胞样品中的细胞核的方法和系统，例如在计算机上进行的。 所述方法包括：接收含细胞样品的原始图像; 将原始图像转换成包括一个或多个核簇的分割图像。 所述方法还包括：对于所述一个或多个核簇中的每一个，获得所述核簇的凸包; 通过将核簇与核簇的凸包进行比较来确定核簇上的任何缺陷; 基于压痕的计数计算第一核计数; 以及将所述核簇分配给所述多个小区中的小区。 所述方法还包括：通过总计其组成核簇的第一核计数来计算每个细胞的第二核计数; 以及基于所述多个单元中的至少一个单元的第二核计数呈现结果。

公开(公告)号：US20130329233A1

公开(公告)日：2013-12-12

申请号：US13491017

申请日：2012-06-07

Applicant: Avrum I. Cohen

Inventor： Avrum I. Cohen

IPC: G01B11/14 ,  G02B21/26

CPC classification number: G01M11/0228 ,  G01B9/04 ,  G02B21/006 ,  G02B21/245 ,  G06F19/10

Abstract: A system and method of determining a focal position for an objective positioned at a measurement location of a sample holder in a microscopy imaging system are provided. The objective is moved to a position relative to the sample holder that corresponds to a distance between the objective and the sample holder. The sample holder has a conditioned upper surface. A focusing light beam is projected onto the sample holder when the objective is located at the position, and the objective focuses the focusing light beam on the sample holder. A reflected light beam resulting from reflection of the focusing light beam off the conditioned upper surface is observed. The focal position for the objective is determined based on the reflected light beam such that the objective produces an in focus image of a microscopy sample when the objective is located at the focal position.

Abstract translation: 提供了一种用于确定位于显微镜成像系统中的样品架的测量位置处的物镜的焦点位置的系统和方法。 将物镜移动到相对于样品架的位置，该位置对应于物镜与样品架之间的距离。 样品架具有调节的上表面。 当物镜位于该位置时，聚焦光束投影到样品架上，并且物镜将聚焦光束聚焦在样品架上。 观察到由聚焦光束从调节的上表面反射而产生的反射光束。 基于反射光束确定目标的焦点位置，使得当物镜位于焦点位置时，物镜产生显微镜样本的聚焦图像。