专利检索 ap:("B. Duane Price" OR "Roland R. Rueckert" OR "Michael A. Krol" OR "Paul G. Ahlquist") AND inv:"Michael A. Krol" 第 1 页

1.

发明授权
Method of producing particles containing nucleic acid sequences in yeast 失效

公开(公告)号：US5869287A

公开(公告)日：1999-02-09

申请号：US682827

申请日：1996-07-12

申请人： B. Duane Price , Roland R. Rueckert , Michael A. Krol , Paul G. Ahlquist

发明人： B. Duane Price , Roland R. Rueckert , Michael A. Krol , Paul G. Ahlquist

IPC分类号： C12N15/09 , C07K14/08 , C12N7/00 , C12N15/81 , C12P21/06

CPC分类号： C07K14/005 , C12N15/81 , C12N2770/16022 , C12N2770/30022

摘要： A method of synthesizing particles containing nucleic acid sequences is disclosed. This method comprises the steps of introducing a non-endogenous packageable nucleic acid sequence and a sequence encoding a suitable non-endogenous capsid protein into yeast cells and inducing particle assembly, wherein at least one nucleic acid is encapsidated. Preferably, the particle is an infectious virion and the method additionally comprises the step of inducing viral nucleic acid replication before particle assembly. A method of replicating an animal or Nodaviridae virus nucleic acid sequences in yeast is also disclosed.