利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

23 条记录 (耗时 0.018 秒)

    Methods and molecules for suppression of RNA silencing
    1.
    发明授权
    Methods and molecules for suppression of RNA silencing 有权

    公开(公告)号：US09644188B2

    公开(公告)日：2017-05-09

    申请号：US14254886

    申请日：2014-04-16

    申请人： Aln Rao ,  Jang-Kyung Seo

    发明人： Aln Rao ,  Jang-Kyung Seo

    IPC分类号： C12N7/00 ,  C12N7/02 ,  A61K39/12 ,  C07K14/005

    CPC分类号： C12N7/00 ,  C07K14/005 ,  C12N7/025 ,  C12N2770/30022 ,  C12N2770/30043 ,  C12N2770/30052

    摘要： Certain embodiments of the invention relate to mutant forms of flock house virus B2 protein characterized by having enhanced suppressor of RNA silencing activity as compared to wild type flock house virus B2 protein. Certain embodiments of the invention relate to nucleic acid sequences and vectors that encode and/or direct expression of such mutant forms of flock house virus B2 protein in eukaryotic cells. Certain embodiments of the invention relate to methods of using such mutant forms of flock house virus B2 protein and/or nucleic acid sequences and vectors that encode and/or direct expression thereof in eukaryotic cells to increase a replication rate of a plant or animal virus in a plant or animal cell.

    METHOD OF PRODUCING PARTICLES CONTAINING NUCLEIC ACIDS SEQUENCES IN YEAST
    9.
    发明申请
    METHOD OF PRODUCING PARTICLES CONTAINING NUCLEIC ACIDS SEQUENCES IN YEAST 无效
    在YEAST中生产含有核酸序列的颗粒的方法

    公开(公告)号：US20020072094A1

    公开(公告)日：2002-06-13

    申请号：US09124600

    申请日：1998-07-29

    发明人： B. DUANE PRICE ,  ROLAND R. RUECKERT ,  MICHAEL A. KROL ,  PAUL G. AHLQUIST

    IPC分类号： C12P019/34 ,  C12N007/00

    CPC分类号： C07K14/005 ,  C12N15/81 ,  C12N2770/16022 ,  C12N2770/30022

    摘要： A method of synthesizing particles containing nucleic acid sequences is disclosed. This method comprises the steps of introducing a non-endogenous packageable nucleic acid sequence and a sequence encoding a suitable non-endogenous capsid protein into yeast cells and inducing particle assembly, wherein at least one nucleic acid is encapsidated. Preferably, the particle is an infectious virion and the method additionally comprises the step of inducing viral nucleic acid replication before particle assembly. A method of replicating an animal or Nodaviridae virus nucleic acid sequences in yeast is also disclosed.

    摘要翻译： 公开了一种合成含有核酸序列的颗粒的方法。 该方法包括以下步骤：向酵母细胞中引入非内源可包装的核酸序列和编码合适的非内源衣壳蛋白的序列，并诱导其中至少一个核酸被包被。 优选地，该颗粒是感染性病毒体，并且该方法另外包括在颗粒组装之前诱导病毒核酸复制的步骤。 还公开了在酵母中复制动物或者Nodaviridae病毒核酸序列的方法。