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公开(公告)号：US20230183735A1
公开(公告)日：2023-06-15
申请号：US17768157
申请日：2020-10-12
发明人： Amanda Marie Garbers , Duane Lehtinen , Nanasaheb Chougule , Aaron Beyerlein , Jelena Zaitseva , Timothy Eberle , Lei Ding
IPC分类号： C12N15/82 , C07K14/325 , C12N15/75 , A01N63/23 , A01P7/04
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/75 , A01N63/23 , A01P7/04
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO: 16 to 30, or the nucleotide sequence set forth in any of SEQ ID NO: 1 to 15, as well as variants and fragments thereof.
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公开(公告)号：US20230242935A1
公开(公告)日：2023-08-03
申请号：US17768984
申请日：2020-10-12
发明人： Amanda Marie Garbers , Nanasaheb Chougule , Jelena Zaitseva , Duane Lehtinen , Aaron Beyerlein , Timothy Eberle , Lei Ding
IPC分类号： C12N15/82
CPC分类号： C12N15/8286
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO: 19 to 36, or the nucleotide sequence set forth in any of SEQ ID NO: 1 to 18, as well as variants and fragments thereof.
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