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公开(公告)号：US11279946B2
公开(公告)日：2022-03-22
申请号：US16478236
申请日：2018-01-18
发明人： Gabriel Miller , Ethan Dunn , James Doroghazi , Duane Lehtinen , Laura Schouten , Andrew Debrecht , Jonathan Giebel , Daniel Vaknin , Xunhai Zheng , Kathleen Pitcher
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO:1-40, 42, 43, 45-48, 50, 51, 52, 54, 56, 58-64, 66, or 67, or the nucleotide sequence set forth in any of SEQ ID NO:69-106, as well as variants and fragments thereof.
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公开(公告)号：US11091772B2
公开(公告)日：2021-08-17
申请号：US16463096
申请日：2017-11-22
IPC分类号： C12N15/82 , C07K14/325
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.
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公开(公告)号：US10435707B2
公开(公告)日：2019-10-08
申请号：US15768237
申请日：2016-10-13
发明人： Elyse Rodgers-Vieira , Kimberly Sampson , Duane Lehtinen , Nanasaheb Chougule , Peter Loesel , Daniela Portz
IPC分类号： C12N15/82 , C07K14/21 , C07K14/195 , C12N15/62
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4-11, or the nucleotide sequence set forth in SEQ ID NO: 1-3, as well as variants and fragments thereof.
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公开(公告)号：US20230183735A1
公开(公告)日：2023-06-15
申请号：US17768157
申请日：2020-10-12
发明人： Amanda Marie Garbers , Duane Lehtinen , Nanasaheb Chougule , Aaron Beyerlein , Jelena Zaitseva , Timothy Eberle , Lei Ding
IPC分类号： C12N15/82 , C07K14/325 , C12N15/75 , A01N63/23 , A01P7/04
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/75 , A01N63/23 , A01P7/04
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO: 16 to 30, or the nucleotide sequence set forth in any of SEQ ID NO: 1 to 15, as well as variants and fragments thereof.
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公开(公告)号：US11286498B2
公开(公告)日：2022-03-29
申请号：US16478359
申请日：2018-01-18
发明人： Kathleen Pitcher , Gabriel Miller , Ethan Dunn , James Doroghazi , Daniel Vaknin , Xunhai Zheng , Duane Lehtinen , Laura Schouten , Andrew Debrecht , Jonathan Giebel
摘要： Compositions and methods for conferring resistance to a plant pathogen are provided. Compositions comprising a coding sequence for a polypeptide having antifungal activity are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO:1-67, or the nucleotide sequence set forth in any of SEQ ID NO:69-81, 83-95, or 97-106, as well as variants and fragments thereof.
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公开(公告)号：US20190276841A1
公开(公告)日：2019-09-12
申请号：US16463096
申请日：2017-11-22
IPC分类号： C12N15/82 , C07K14/325
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.
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公开(公告)号：US20230242935A1
公开(公告)日：2023-08-03
申请号：US17768984
申请日：2020-10-12
发明人： Amanda Marie Garbers , Nanasaheb Chougule , Jelena Zaitseva , Duane Lehtinen , Aaron Beyerlein , Timothy Eberle , Lei Ding
IPC分类号： C12N15/82
CPC分类号： C12N15/8286
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO: 19 to 36, or the nucleotide sequence set forth in any of SEQ ID NO: 1 to 18, as well as variants and fragments thereof.
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公开(公告)号：US20230115343A1
公开(公告)日：2023-04-13
申请号：US17904715
申请日：2021-02-19
发明人： Nanasaheb Chougule , Margaret Dooley , Jelena Zaitseva , Duane Lehtinen , Timothy Eberle , Lei Ding , Yasmin Cardoza
IPC分类号： C12N15/82
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO: 22 to 42, or the nucleotide sequence set forth in any of SEQ ID NO: 1 to 21, as well as variants and fragments thereof.
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