公开(公告)号：US20200181627A1

公开(公告)日：2020-06-11

申请号：US16631999

申请日：2018-07-11

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Bogdan Tokovenko

IPC: C12N15/70 ,  C12N15/74

Abstract: The present invention is directed to a nucleic acid construct comprising a polynucleotide operably linked to one or more control sequence that directs the expression of the polynucleotide in a host cell, wherein at least one control sequence comprises a promoter sequence of an operon comprising a secA gene or a functional fragment or functional variant thereof and wherein said promoter sequence is heterologous to the polynucleotide. In a further embodiment, the invention is directed to an expression vector and a host cell comprising the nucleic acid construct comprising the promoter sequence described herein and a method of expressing a polynucleotide in a host cell.

公开(公告)号：US20250008961A1

公开(公告)日：2025-01-09

申请号：US18711970

申请日：2022-11-11

Applicant: BASF SE

Inventor： Tobias May ,  Judith Zimmermann ,  Bogdan Tokovenko ,  Daniel Christoph Heinrich ,  Reinhard Stierl ,  Andrea Herold

IPC: A01N63/25 ,  A01P3/00 ,  C12N1/20 ,  C12N9/10

Abstract: The present invention relates to Paenibacillus species strains producing low amounts of com-pounds requiring L-2,4-diaminobutanoate as precursor and exopolysaccharides comprising low amounts of compounds requiring L-2,4-diaminobutanoate as precursor, as well as mixtures, compositions and methods comprising these Paenibacillus species strains and/or exopolysac-charides.

公开(公告)号：US12065652B2

公开(公告)日：2024-08-20

申请号：US16631999

申请日：2018-07-11

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Bogdan Tokovenko

IPC: C12N15/70 ,  C12N15/74

CPC classification number: C12N15/70 ,  C12N15/74 ,  C12N2800/101 ,  C12N2800/70 ,  C12N2830/005 ,  C12N2830/34 ,  C12N2830/55

Abstract: The present invention is directed to a nucleic acid construct comprising a polynucleotide operably linked to one or more control sequence that directs the expression of the polynucleotide in a host cell, wherein at least one control sequence comprises a promoter sequence of an operon comprising a secA gene or a functional fragment or functional variant thereof and wherein said promoter sequence is heterologous to the polynucleotide. In a further embodiment, the invention is directed to an expression vector and a host cell comprising the nucleic acid construct comprising the promoter sequence described herein and a method of expressing a polynucleotide in a host cell.