公开(公告)号：US20230399589A1

公开(公告)日：2023-12-14

申请号：US18178328

申请日：2023-03-03

Applicant: BASF SE

Inventor： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

IPC: C11D3/386 ,  C12N9/54 ,  C12N9/42

CPC classification number: C11D3/38663 ,  C11D3/38645 ,  C12N9/54 ,  C11D3/38618 ,  C12N9/2437

Abstract: According to the invention, storage stability in terms of cellulolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and cellulase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.

公开(公告)号：US20180155659A1

公开(公告)日：2018-06-07

申请号：US15804507

申请日：2017-11-06

Applicant: BASF SE

Inventor： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

IPC: C11D3/386 ,  C12N9/54 ,  C12N9/42

CPC classification number: C11D3/38663 ,  C11D3/38618 ,  C11D3/38645 ,  C12N9/2437 ,  C12N9/54

Abstract: According to the invention, storage stability in terms of cellulolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and cellulase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.

公开(公告)号：US20240018447A1

公开(公告)日：2024-01-18

申请号：US18178360

申请日：2023-03-03

Applicant: BASF SE

Inventor： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell

IPC: C11D3/386 ,  C12N9/54

CPC classification number: C11D3/38618 ,  C11D3/38627 ,  C11D3/38681 ,  C12N9/54

Abstract: The object is to improve the storage stability of a liquid washing or cleaning agent comprising a protease and lipase with regard to lipolytic activity. This is achieved through the use of a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the sequence corresponding to SEQ ID NO. 1.

公开(公告)号：US20180163160A1

公开(公告)日：2018-06-14

申请号：US15819901

申请日：2017-11-21

Applicant: BASF SE

Inventor： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell

IPC: C11D3/386 ,  C12N9/54

CPC classification number: C11D3/38618 ,  C11D3/38627 ,  C11D3/38681 ,  C12N9/54

Abstract: The object is to improve the storage stability of a liquid washing or cleaning agent comprising a protease and lipase with regard to lipolytic activity. This is achieved through the use of a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the sequence corresponding to SEQ ID NO. 1.

公开(公告)号：US20180127684A1

公开(公告)日：2018-05-10

申请号：US15793576

申请日：2017-10-25

Applicant: BASF SE

Inventor： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

IPC: C11D3/386 ,  C12N9/54

Abstract: According to the invention, storage stability in terms of amylolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and amylase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.

公开(公告)号：US09353361B2

公开(公告)日：2016-05-31

申请号：US14300826

申请日：2014-06-10

Applicant: BASF SE

Inventor： Cornelius Bessler ,  Susanne Wieland ,  Karl-Heinz Maurer

IPC: C12N15/56 ,  C12N9/28 ,  C11D3/386

CPC classification number: C12N9/2417 ,  C11D3/38681

Abstract: The present invention relates to α-amylase variants that are stabilized to dimerization and/or multimerization, in particular at elevated temperatures or high pH, by point mutagenesis of positively polarized or charged or neutral surface amino acids to give more negatively polarized or charged amino acids. The invention further relates to methods of increasing the stability of an α-amylase to dimerization and/or multimerization brought about by electrostatic interactions whereby at least one amino acid residue on the surface of the starting molecule, which makes a neutral or positively polar or charged contribution to the electrostatic potential of said molecule, is replaced with a more negatively polar or negatively charged amino acid residue. The α-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.

Abstract translation: 本发明涉及通过正极化或带电荷或中性表面氨基酸的点诱变稳定至二聚化和/或多聚化的α-淀粉酶变体，特别是在升高的温度或高pH下，以产生更多的负极化或带电荷的氨基酸 。 本发明还涉及增加α-淀粉酶对由静电相互作用引起的二聚化和/或多聚化的稳定性的方法，其中起始分子表面上至少有一个氨基酸残基，其形成中性或正极性或带电 对所述分子的静电电位的贡献被更负极性或带负电荷的氨基酸残基取代。 由此获得的α-淀粉酶变体对溶剂的影响表现出更好的稳定性，增加的持续性，并且适用于许多工业使用领域，特别是作为洗涤剂和清洁剂中的活性成分。

公开(公告)号：US20230365898A1

公开(公告)日：2023-11-16

申请号：US18173620

申请日：2023-02-23

Applicant: BASF SE

Inventor： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

IPC: C11D3/386 ,  C12N9/54

CPC classification number: C11D3/38618 ,  C11D3/38681 ,  C12N9/54

Abstract: According to the invention, storage stability in terms of amylolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and amylase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.