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公开(公告)号：US20230399589A1
公开(公告)日：2023-12-14
申请号：US18178328
申请日：2023-03-03
Applicant: BASF SE
Inventor： Susanne Wieland , Karl-Heinz Maurer , Timothy O'Connell , Hendrik Hellmuth
CPC classification number: C11D3/38663 , C11D3/38645 , C12N9/54 , C11D3/38618 , C12N9/2437
Abstract: According to the invention, storage stability in terms of cellulolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and cellulase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.
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公开(公告)号：US11046961B2
公开(公告)日：2021-06-29
申请号：US16700322
申请日：2019-12-02
Applicant: BASF SE
Inventor： Christian Degering , Thorsten Eggert , Stefan Evers , Karl-Heinz Maurer , Johannes Bongaerts
Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.
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公开(公告)号：US20230365898A1
公开(公告)日：2023-11-16
申请号：US18173620
申请日：2023-02-23
Applicant: BASF SE
Inventor： Susanne Wieland , Karl-Heinz Maurer , Timothy O'Connell , Hendrik Hellmuth
CPC classification number: C11D3/38618 , C11D3/38681 , C12N9/54
Abstract: According to the invention, storage stability in terms of amylolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and amylase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.
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公开(公告)号：US20180073005A1
公开(公告)日：2018-03-15
申请号：US15712652
申请日：2017-09-22
Applicant: BASF SE
Inventor： Christian Degering , Thorsten Eggert , Stefan Evers , Karl-Heinz Maurer , Johannes Bongaerts
CPC classification number: C12N9/50 , C12N15/625 , C12N15/70 , C12N15/75
Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.
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公开(公告)号：US09725704B2
公开(公告)日：2017-08-08
申请号：US14374556
申请日：2013-01-29
Applicant: BASF SE
Inventor： Tobias Kueppers , Victoria Steffen , Renee Charlott Eichstadt , Stefan Evers , Karl-Heinz Maurer , Johannes Bongaerts , Hendrik Hellmuth , Thomas Weber , Timothy O'Connell
IPC: C12N9/26 , C12N9/50 , C12N1/20 , C12N15/74 , C12P21/06 , C12P19/34 , C12N15/75 , C12P21/02 , C12N9/00 , C12N9/54
CPC classification number: C12N9/2411 , C12N9/00 , C12N9/2414 , C12N9/50 , C12N9/54 , C12N15/75 , C12P21/02
Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.
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Patent Agency Ranking
公开(公告)号：US20180155659A1
公开(公告)日：2018-06-07
申请号：US15804507
申请日：2017-11-06
Applicant: BASF SE
Inventor： Susanne Wieland , Karl-Heinz Maurer , Timothy O'Connell , Hendrik Hellmuth
CPC classification number: C11D3/38663 , C11D3/38618 , C11D3/38645 , C12N9/2437 , C12N9/54
Abstract: According to the invention, storage stability in terms of cellulolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and cellulase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.
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公开(公告)号：US20140370569A1
公开(公告)日：2014-12-18
申请号：US14374556
申请日：2013-01-29
Applicant: BASF SE
Inventor： Tobias Kueppers , Victoria Steffen , Renee Charlott Eichstadt , Stefan Evers , Karl-Heinz Maurer , Johannes Bongaerts , Hendrik Hellmuth , Thomas Weber , Timothy O'Connell
CPC classification number: C12N9/2411 , C12N9/00 , C12N9/2414 , C12N9/50 , C12N9/54 , C12N15/75 , C12P21/02
Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.
Abstract translation: 在微生物发酵中，目的是提高蛋白质的产率。 这是通过一种方法实现的，其中将表达构建体引入到包含启动子和编码该蛋白质的核酸的物种短小芽孢杆菌的微生物中，并且所述蛋白质在所述表达构建体中表达。
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公开(公告)号：US20240018447A1
公开(公告)日：2024-01-18
申请号：US18178360
申请日：2023-03-03
Applicant: BASF SE
Inventor： Susanne Wieland , Karl-Heinz Maurer , Timothy O'Connell
CPC classification number: C11D3/38618 , C11D3/38627 , C11D3/38681 , C12N9/54
Abstract: The object is to improve the storage stability of a liquid washing or cleaning agent comprising a protease and lipase with regard to lipolytic activity. This is achieved through the use of a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the sequence corresponding to SEQ ID NO. 1.
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公开(公告)号：US20180163160A1
公开(公告)日：2018-06-14
申请号：US15819901
申请日：2017-11-21
Applicant: BASF SE
Inventor： Susanne Wieland , Karl-Heinz Maurer , Timothy O'Connell
CPC classification number: C11D3/38618 , C11D3/38627 , C11D3/38681 , C12N9/54
Abstract: The object is to improve the storage stability of a liquid washing or cleaning agent comprising a protease and lipase with regard to lipolytic activity. This is achieved through the use of a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the sequence corresponding to SEQ ID NO. 1.
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公开(公告)号：US20180127684A1
公开(公告)日：2018-05-10
申请号：US15793576
申请日：2017-10-25
Applicant: BASF SE
Inventor： Susanne Wieland , Karl-Heinz Maurer , Timothy O'Connell , Hendrik Hellmuth
Abstract: According to the invention, storage stability in terms of amylolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and amylase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.
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